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Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.


Real-time PCR analysis of AtSPS (At5g20280) and AtSPP (At2g35750) gene expression in rosette leaves of Arabidopsis indicates increased expression of both transgenes when compared with the Atsps– mutant. AtSPS (At5g20280) was tagged with hRLUC in N- and C-terminal orientations (SPSR and RSPS), and AtSPP (At2g35740) was tagged with YFP at the C-terminus (SPPY). The Arabidopsis ubiqutin5 gene was used as an internal control, and results are given as relative transgene transcript abundance.
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Figure 6: Real-time PCR analysis of AtSPS (At5g20280) and AtSPP (At2g35750) gene expression in rosette leaves of Arabidopsis indicates increased expression of both transgenes when compared with the Atsps– mutant. AtSPS (At5g20280) was tagged with hRLUC in N- and C-terminal orientations (SPSR and RSPS), and AtSPP (At2g35740) was tagged with YFP at the C-terminus (SPPY). The Arabidopsis ubiqutin5 gene was used as an internal control, and results are given as relative transgene transcript abundance.

Mentions: Double transgenic Arabidopsis plants in the Atsps– mutant background were analysed by quantitative RT-PCR to assess the transcript abundance of each cisgene. AtSPS and AtSPP transcripts was quantified in rosette leaf tissue for all transformed lines and normalized to AtUBQ5 abundance (Fig. 6). No AtSPS transcript was observed in the Atsps– mutant as expected, and therefore all transcripts measured were due to the expression of the AtSPS cisgene. Interestingly, the AtSPP transcript level in the Atsps– mutant plant was also reduced compared with wild-type plants. In all cisgenics, the AtSPS transcript abundance was apparent, and the transcript abundance of AtSPP was similar among all lines, which was slightly higher than in the Atsps– mutant plants.


Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Real-time PCR analysis of AtSPS (At5g20280) and AtSPP (At2g35750) gene expression in rosette leaves of Arabidopsis indicates increased expression of both transgenes when compared with the Atsps– mutant. AtSPS (At5g20280) was tagged with hRLUC in N- and C-terminal orientations (SPSR and RSPS), and AtSPP (At2g35740) was tagged with YFP at the C-terminus (SPPY). The Arabidopsis ubiqutin5 gene was used as an internal control, and results are given as relative transgene transcript abundance.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493782&req=5

Figure 6: Real-time PCR analysis of AtSPS (At5g20280) and AtSPP (At2g35750) gene expression in rosette leaves of Arabidopsis indicates increased expression of both transgenes when compared with the Atsps– mutant. AtSPS (At5g20280) was tagged with hRLUC in N- and C-terminal orientations (SPSR and RSPS), and AtSPP (At2g35740) was tagged with YFP at the C-terminus (SPPY). The Arabidopsis ubiqutin5 gene was used as an internal control, and results are given as relative transgene transcript abundance.
Mentions: Double transgenic Arabidopsis plants in the Atsps– mutant background were analysed by quantitative RT-PCR to assess the transcript abundance of each cisgene. AtSPS and AtSPP transcripts was quantified in rosette leaf tissue for all transformed lines and normalized to AtUBQ5 abundance (Fig. 6). No AtSPS transcript was observed in the Atsps– mutant as expected, and therefore all transcripts measured were due to the expression of the AtSPS cisgene. Interestingly, the AtSPP transcript level in the Atsps– mutant plant was also reduced compared with wild-type plants. In all cisgenics, the AtSPS transcript abundance was apparent, and the transcript abundance of AtSPP was similar among all lines, which was slightly higher than in the Atsps– mutant plants.

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.