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Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.


Complementation of the Atspp– mutant line indicates functionality of each BRET construct. (A) Wild type, (B) Atspp– mutant, (C) SPPY in the Atspp– mutant, and (D) RSPS-SPPY in the Atspp– mutant.
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Figure 2: Complementation of the Atspp– mutant line indicates functionality of each BRET construct. (A) Wild type, (B) Atspp– mutant, (C) SPPY in the Atspp– mutant, and (D) RSPS-SPPY in the Atspp– mutant.

Mentions: To support the yeast two-hybrid results, the capacity of AtSPS and AtSPP to interact in planta was studied using the BRET technique. The functionality of each BRET construct described previously was confirmed by complementation of the dwarf phenotype of the respective mutants (Figs 2, 8). Four double transgenic lines were then created by co-transforming either the Atsps– mutant or the Atspp– mutant with either the 35SP::AtSPS-hRLUC or 35SP::hRLUC::AtSPS construct and the 35SP::AtSPP–YFP construct, respectively. Before the BRET assay was conducted, the double transgenic lines were screened by genomic DNA PCR and subsequently YFP expression was examined by fluorescence microscopy. YFP fluorescent signals were observed clearly in most of the root tissue, excluding the root tip region in the transgenic plants, providing further confirmation that the constructs were functional, as well as providing proof that YFP fusion proteins can be expressed stably in Arabidopsis seedlings (Fig. 3).


Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

Maloney VJ, Park JY, Unda F, Mansfield SD - J. Exp. Bot. (2015)

Complementation of the Atspp– mutant line indicates functionality of each BRET construct. (A) Wild type, (B) Atspp– mutant, (C) SPPY in the Atspp– mutant, and (D) RSPS-SPPY in the Atspp– mutant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493782&req=5

Figure 2: Complementation of the Atspp– mutant line indicates functionality of each BRET construct. (A) Wild type, (B) Atspp– mutant, (C) SPPY in the Atspp– mutant, and (D) RSPS-SPPY in the Atspp– mutant.
Mentions: To support the yeast two-hybrid results, the capacity of AtSPS and AtSPP to interact in planta was studied using the BRET technique. The functionality of each BRET construct described previously was confirmed by complementation of the dwarf phenotype of the respective mutants (Figs 2, 8). Four double transgenic lines were then created by co-transforming either the Atsps– mutant or the Atspp– mutant with either the 35SP::AtSPS-hRLUC or 35SP::hRLUC::AtSPS construct and the 35SP::AtSPP–YFP construct, respectively. Before the BRET assay was conducted, the double transgenic lines were screened by genomic DNA PCR and subsequently YFP expression was examined by fluorescence microscopy. YFP fluorescent signals were observed clearly in most of the root tissue, excluding the root tip region in the transgenic plants, providing further confirmation that the constructs were functional, as well as providing proof that YFP fusion proteins can be expressed stably in Arabidopsis seedlings (Fig. 3).

Bottom Line: Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins.The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch.Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar.

View Article: PubMed Central - PubMed

Affiliation: Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.

No MeSH data available.