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Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize (Zea mays L.) lines.

Li M, Heckwolf M, Crowe JD, Williams DL, Magee TD, Kaeppler SM, de Leon N, Hodge DB - J. Exp. Bot. (2015)

Bottom Line: The hydrolysis yield following pre-treatment exhibited statistically significant negative correlations to the lignin content after pre-treatment and positive correlations to the solubilized ferulic acid and pCA.Several unanticipated results were observed, including a positive correlation between initial lignin and acetate content, lack of correlation between acetate content and initial xylan content, and negative correlation between each of these three variables to the hydrolysis yields for untreated maize.Another surprising result was that pCA release was negatively correlated with hydrolysis yields for untreated maize and, along with ferulic acid release, was positively correlated with the pre-treated maize hydrolysis yields.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems & Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA DOE-Great Lakes Bioenergy Research Center, 1552 University Ave., Madison, WI 53703, USA.

No MeSH data available.


Related in: MedlinePlus

Several between-property correlations highlighted to demonstrate relationships within the data set. Each data point represents the property for one of the 27 maize lines. Pearson’s correlation coefficients and P values are presented for all property correlations with P≤0.05. Error bars on individual samples are not shown to improve clarity. (This figure is available in colour at JXB online.)
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Figure 3: Several between-property correlations highlighted to demonstrate relationships within the data set. Each data point represents the property for one of the 27 maize lines. Pearson’s correlation coefficients and P values are presented for all property correlations with P≤0.05. Error bars on individual samples are not shown to improve clarity. (This figure is available in colour at JXB online.)

Mentions: In order to visualize better the relationships between the variables, a correlation map was developed and organized using hierarchical clustering according to the Euclidian distance between sets of the Pearson correlation coefficients (R) (Fig. 2). The magnitude, scale, and significance of the correlations are presented in Supplementary Table S2 at JXB online. Within the correlation map (Fig. 2), several multi-property clusters of positive correlations were observed along the top left to bottom right diagonal, while one multi-property cluster of negative correlations stood out in both the bottom left and top right corners. This indicated that there a number of properties that are correlated across diverse maize lines and may be responsible for differences in the cell wall’s response to enzymatic hydrolysis as well as the response to pre-treatment. A number of strong positive correlations between related properties or yields were observable along the top left to bottom right diagonal, namely the initial 6 and final 72h hydrolysis yields, initial pCA content and pCA solubilization, initial FA content and FA solubilization, and initial and final WRV. These specific results are not surprising as they may be expected to be related. A number of property correlations that highlight either causal or merely correlative relationships between cell-wall properties (but not hydrolysis yields) from within the highlighted clusters were selected and replotted in Fig. 3. These correlations were plotted using either initial acetate content or initial WRV as the abscissa, as these properties appeared within two of the important clusters. It should be stressed that acetate content and WRV are not necessarily the properties responsible for the variations in the other properties but were merely correlated to them.


Cell-wall properties contributing to improved deconstruction by alkaline pre-treatment and enzymatic hydrolysis in diverse maize (Zea mays L.) lines.

Li M, Heckwolf M, Crowe JD, Williams DL, Magee TD, Kaeppler SM, de Leon N, Hodge DB - J. Exp. Bot. (2015)

Several between-property correlations highlighted to demonstrate relationships within the data set. Each data point represents the property for one of the 27 maize lines. Pearson’s correlation coefficients and P values are presented for all property correlations with P≤0.05. Error bars on individual samples are not shown to improve clarity. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4493778&req=5

Figure 3: Several between-property correlations highlighted to demonstrate relationships within the data set. Each data point represents the property for one of the 27 maize lines. Pearson’s correlation coefficients and P values are presented for all property correlations with P≤0.05. Error bars on individual samples are not shown to improve clarity. (This figure is available in colour at JXB online.)
Mentions: In order to visualize better the relationships between the variables, a correlation map was developed and organized using hierarchical clustering according to the Euclidian distance between sets of the Pearson correlation coefficients (R) (Fig. 2). The magnitude, scale, and significance of the correlations are presented in Supplementary Table S2 at JXB online. Within the correlation map (Fig. 2), several multi-property clusters of positive correlations were observed along the top left to bottom right diagonal, while one multi-property cluster of negative correlations stood out in both the bottom left and top right corners. This indicated that there a number of properties that are correlated across diverse maize lines and may be responsible for differences in the cell wall’s response to enzymatic hydrolysis as well as the response to pre-treatment. A number of strong positive correlations between related properties or yields were observable along the top left to bottom right diagonal, namely the initial 6 and final 72h hydrolysis yields, initial pCA content and pCA solubilization, initial FA content and FA solubilization, and initial and final WRV. These specific results are not surprising as they may be expected to be related. A number of property correlations that highlight either causal or merely correlative relationships between cell-wall properties (but not hydrolysis yields) from within the highlighted clusters were selected and replotted in Fig. 3. These correlations were plotted using either initial acetate content or initial WRV as the abscissa, as these properties appeared within two of the important clusters. It should be stressed that acetate content and WRV are not necessarily the properties responsible for the variations in the other properties but were merely correlated to them.

Bottom Line: The hydrolysis yield following pre-treatment exhibited statistically significant negative correlations to the lignin content after pre-treatment and positive correlations to the solubilized ferulic acid and pCA.Several unanticipated results were observed, including a positive correlation between initial lignin and acetate content, lack of correlation between acetate content and initial xylan content, and negative correlation between each of these three variables to the hydrolysis yields for untreated maize.Another surprising result was that pCA release was negatively correlated with hydrolysis yields for untreated maize and, along with ferulic acid release, was positively correlated with the pre-treated maize hydrolysis yields.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems & Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA DOE-Great Lakes Bioenergy Research Center, 1552 University Ave., Madison, WI 53703, USA.

No MeSH data available.


Related in: MedlinePlus