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Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression.

Clark LV, Stewart JR, Nishiwaki A, Toma Y, Kjeldsen JB, Jørgensen U, Zhao H, Peng J, Yoo JH, Heo K, Yu CY, Yamada T, Sacks EJ - J. Exp. Bot. (2015)

Bottom Line: Unexpectedly, rare (~1%) diploid M. sinensis individuals from northern Japan were found with 6-27% M. sacchariflorus ancestry.In contrast to limited introgression between diploid M. sacchariflorus and M. sinensis in northern China, selection for adaptation to a moderate maritime climate probably favoured cross-ploidy introgressants in southern Japan.These results will help guide the selection of Miscanthus accessions for the breeding of biomass cultivars.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Sciences, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA.

No MeSH data available.


Related in: MedlinePlus

Photographs of EBI-2009-02c, an M. sinensis×M. sacchariflorus individual grown from seed collected in Hokkaido, Japan. Ancestry of EBI-2009-02c according to Structure was ~73% M. sinensis from N Japan and ~27% M. sacchariflorus (Fig. 1A, C, E). Its plastid haplotype was commonly found among M. sinensis in Hokkaido (haplotype C, Fig. 4). (A) Close-up showing axillary branching and long internodes, which are characteristic of M. sacchariflorus. (B) Broader view, with more branching visible. (C–E) Abaxial leaf surface of three plants, showing presence or absence of trichomes. Scale is identical in C–E. (C) Non-hybrid M. sinensis displaying trichomes (arrow), which is typical for this species. (D) EBI-2009-02c, with trichomes (arrow). (E) Diploid non-hybrid M. sacchariflorus, with a glabrous phenotype that is typical of this species.
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Figure 2: Photographs of EBI-2009-02c, an M. sinensis×M. sacchariflorus individual grown from seed collected in Hokkaido, Japan. Ancestry of EBI-2009-02c according to Structure was ~73% M. sinensis from N Japan and ~27% M. sacchariflorus (Fig. 1A, C, E). Its plastid haplotype was commonly found among M. sinensis in Hokkaido (haplotype C, Fig. 4). (A) Close-up showing axillary branching and long internodes, which are characteristic of M. sacchariflorus. (B) Broader view, with more branching visible. (C–E) Abaxial leaf surface of three plants, showing presence or absence of trichomes. Scale is identical in C–E. (C) Non-hybrid M. sinensis displaying trichomes (arrow), which is typical for this species. (D) EBI-2009-02c, with trichomes (arrow). (E) Diploid non-hybrid M. sacchariflorus, with a glabrous phenotype that is typical of this species.

Mentions: Based on admixture estimates, M. sinensis genotypes in Japan were strongly isolated from each of the other five groups identified (Fig. 1A, Supplementary Dataset S1). Isolation of M. sinensis from M. sacchariflorus in Japan was especially strong. Only 9 of the 667 phenotypically M. sinensis genotypes evaluated had <99% M. sinensis ancestry. Unexpectedly, however, four diploid individuals from Hokkaido and one from Ibaraki (central Honshu) had hybrid ancestry >5% from M. sacchariflorus (27%, 18%, 14%, 6%, and 9% respectively), and were part of seed accessions that were otherwise non-hybrid (EBI-2009-02c, Koike-05a, EBI-2008-46c, EBI-2008-37e, JA55-2c; Supplementary dataset S1). For EBI-2009-02c, intermediate morphological characteristics were observed between M. sacchariflorus and M. sinensis, including axillary branching, which is characteristic of M. sacchariflorus, and trichomes on the abaxial surface of leaves, which is characteristic of M. sinensis (Fig. 2). Among the Japanese genotypes with ≥99% M. sinensis ancestry, only 39 out of 795 had less than 95% Japanese ancestry. Most of the non-Japanese admixture observed for M. sinensis from Japan was with the southeast (SE) China M. sinensis group (Fig. 1A, Supplementary Dataset S1).


Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression.

Clark LV, Stewart JR, Nishiwaki A, Toma Y, Kjeldsen JB, Jørgensen U, Zhao H, Peng J, Yoo JH, Heo K, Yu CY, Yamada T, Sacks EJ - J. Exp. Bot. (2015)

Photographs of EBI-2009-02c, an M. sinensis×M. sacchariflorus individual grown from seed collected in Hokkaido, Japan. Ancestry of EBI-2009-02c according to Structure was ~73% M. sinensis from N Japan and ~27% M. sacchariflorus (Fig. 1A, C, E). Its plastid haplotype was commonly found among M. sinensis in Hokkaido (haplotype C, Fig. 4). (A) Close-up showing axillary branching and long internodes, which are characteristic of M. sacchariflorus. (B) Broader view, with more branching visible. (C–E) Abaxial leaf surface of three plants, showing presence or absence of trichomes. Scale is identical in C–E. (C) Non-hybrid M. sinensis displaying trichomes (arrow), which is typical for this species. (D) EBI-2009-02c, with trichomes (arrow). (E) Diploid non-hybrid M. sacchariflorus, with a glabrous phenotype that is typical of this species.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 2: Photographs of EBI-2009-02c, an M. sinensis×M. sacchariflorus individual grown from seed collected in Hokkaido, Japan. Ancestry of EBI-2009-02c according to Structure was ~73% M. sinensis from N Japan and ~27% M. sacchariflorus (Fig. 1A, C, E). Its plastid haplotype was commonly found among M. sinensis in Hokkaido (haplotype C, Fig. 4). (A) Close-up showing axillary branching and long internodes, which are characteristic of M. sacchariflorus. (B) Broader view, with more branching visible. (C–E) Abaxial leaf surface of three plants, showing presence or absence of trichomes. Scale is identical in C–E. (C) Non-hybrid M. sinensis displaying trichomes (arrow), which is typical for this species. (D) EBI-2009-02c, with trichomes (arrow). (E) Diploid non-hybrid M. sacchariflorus, with a glabrous phenotype that is typical of this species.
Mentions: Based on admixture estimates, M. sinensis genotypes in Japan were strongly isolated from each of the other five groups identified (Fig. 1A, Supplementary Dataset S1). Isolation of M. sinensis from M. sacchariflorus in Japan was especially strong. Only 9 of the 667 phenotypically M. sinensis genotypes evaluated had <99% M. sinensis ancestry. Unexpectedly, however, four diploid individuals from Hokkaido and one from Ibaraki (central Honshu) had hybrid ancestry >5% from M. sacchariflorus (27%, 18%, 14%, 6%, and 9% respectively), and were part of seed accessions that were otherwise non-hybrid (EBI-2009-02c, Koike-05a, EBI-2008-46c, EBI-2008-37e, JA55-2c; Supplementary dataset S1). For EBI-2009-02c, intermediate morphological characteristics were observed between M. sacchariflorus and M. sinensis, including axillary branching, which is characteristic of M. sacchariflorus, and trichomes on the abaxial surface of leaves, which is characteristic of M. sinensis (Fig. 2). Among the Japanese genotypes with ≥99% M. sinensis ancestry, only 39 out of 795 had less than 95% Japanese ancestry. Most of the non-Japanese admixture observed for M. sinensis from Japan was with the southeast (SE) China M. sinensis group (Fig. 1A, Supplementary Dataset S1).

Bottom Line: Unexpectedly, rare (~1%) diploid M. sinensis individuals from northern Japan were found with 6-27% M. sacchariflorus ancestry.In contrast to limited introgression between diploid M. sacchariflorus and M. sinensis in northern China, selection for adaptation to a moderate maritime climate probably favoured cross-ploidy introgressants in southern Japan.These results will help guide the selection of Miscanthus accessions for the breeding of biomass cultivars.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Sciences, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA.

No MeSH data available.


Related in: MedlinePlus