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Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer.

Borcherding N, Bormann N, Kusner D, Kolb R, Zhang W - Genom Data (2015)

Bottom Line: Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR).Here we provide further details on the experimental methods and microarray analysis.This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Iowa, Iowa City, IA 52242-1109 ; Medical Science Training Program, University of Iowa, Iowa City, IA 52242-1109 ; Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA 52242-1109 ; Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109.

ABSTRACT

Breast cancer is the leading cause of cancer-related mortality for females worldwide.(1) Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in an MMTV-ErbB2 mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplastic MMTV-ErbB2 female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potential even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).

No MeSH data available.


Related in: MedlinePlus

Box plot of the quantile normalized expression level for the 12 microarrays. The line bisecting the boxplot is the mean probe value. Samples appear in the order of series matrix file of GSE64487 dataset, the order of the original blinding of the RNA experiment.
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f0005: Box plot of the quantile normalized expression level for the 12 microarrays. The line bisecting the boxplot is the mean probe value. Samples appear in the order of series matrix file of GSE64487 dataset, the order of the original blinding of the RNA experiment.

Mentions: Beadchips were scanned with the Illumina iScan System and data was collected using GenomeStudio software v2011.1. Microarray data was quantile normalized and transformed into log2 expression by the Iowa Institute of Human Genetics Bioinformatics Division (Fig. 1).


Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer.

Borcherding N, Bormann N, Kusner D, Kolb R, Zhang W - Genom Data (2015)

Box plot of the quantile normalized expression level for the 12 microarrays. The line bisecting the boxplot is the mean probe value. Samples appear in the order of series matrix file of GSE64487 dataset, the order of the original blinding of the RNA experiment.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493742&req=5

f0005: Box plot of the quantile normalized expression level for the 12 microarrays. The line bisecting the boxplot is the mean probe value. Samples appear in the order of series matrix file of GSE64487 dataset, the order of the original blinding of the RNA experiment.
Mentions: Beadchips were scanned with the Illumina iScan System and data was collected using GenomeStudio software v2011.1. Microarray data was quantile normalized and transformed into log2 expression by the Iowa Institute of Human Genetics Bioinformatics Division (Fig. 1).

Bottom Line: Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR).Here we provide further details on the experimental methods and microarray analysis.This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Iowa, Iowa City, IA 52242-1109 ; Medical Science Training Program, University of Iowa, Iowa City, IA 52242-1109 ; Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA 52242-1109 ; Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109.

ABSTRACT

Breast cancer is the leading cause of cancer-related mortality for females worldwide.(1) Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in an MMTV-ErbB2 mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplastic MMTV-ErbB2 female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potential even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).

No MeSH data available.


Related in: MedlinePlus