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Function of a p24 Heterodimer in Morphogenesis and Protein Transport in Penicillium oxalicum.

Wang F, Liu K, Han L, Jiang B, Wang M, Fang X - Sci Rep (2015)

Bottom Line: The results suggested that the p24 heterodimer mediates protein transport, particularly that of cellobiohydrolase.These results suggest that the p24 heterodimer participates in morphogenesis and protein transport.Compared with P. oxalicum Δerp, a greater number of cellular physiological pathways were impaired in ΔerpΔpδ.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, Shandong, China.

ABSTRACT
The lignocellulose degradation capacity of filamentous fungi has been widely studied because of their cellulase hypersecretion. The p24 proteins in eukaryotes serve important functions in this secretory pathway. However, little is known about the functions of the p24 proteins in filamentous fungi. In this study, four p24 proteins were identified in Penicillium oxalicum. Six p24 double-deletion strains were constructed, and further studies were carried out with the ΔerpΔpδ strain. The experimental results suggested that Erp and Pδ form a p24 heterodimer in vivo. This p24 heterodimer participates in important morphogenetic events, including sporulation, hyphal growth, and lateral branching. The results suggested that the p24 heterodimer mediates protein transport, particularly that of cellobiohydrolase. Analysis of the intracellular proteome revealed that the ΔerpΔpδ double mutant is under secretion stress due to attempts to remove proteins that are jammed in the endomembrane system. These results suggest that the p24 heterodimer participates in morphogenesis and protein transport. Compared with P. oxalicum Δerp, a greater number of cellular physiological pathways were impaired in ΔerpΔpδ. This finding may provide new insights into the secretory pathways of filamentous fungi.

No MeSH data available.


Related in: MedlinePlus

Comparison of Lateral Branching Development Patterns Between the Parent and ΔerpΔpδ Strains.Panel a, microscopic observation of hyphae from the parent and ΔerpΔpδ strains. Scale bar: 10 μm. Panel b, statistical comparison of angle distribution between main hyphae and lateral branches in the parent and ΔerpΔpδ strains. White represents the parent strain, and black represents the ΔerpΔpδ strain. Error bars indicate the standard deviation calculated from three biological experiments.
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f4: Comparison of Lateral Branching Development Patterns Between the Parent and ΔerpΔpδ Strains.Panel a, microscopic observation of hyphae from the parent and ΔerpΔpδ strains. Scale bar: 10 μm. Panel b, statistical comparison of angle distribution between main hyphae and lateral branches in the parent and ΔerpΔpδ strains. White represents the parent strain, and black represents the ΔerpΔpδ strain. Error bars indicate the standard deviation calculated from three biological experiments.

Mentions: An increased branch frequency with dichotomous tips was observed for T. reesei Δerp but not P. oxalicum Δerp when the strains were cultivated on agar plates containing 2% glucose. This indicates that the branching mechanism was disrupted in T. reesei Δerp. To test whether branching was affected in the ΔerpΔpδ strain, approximately 1 × 103 spores were inoculated on glass slides covered by glucose-containing agar media. There are two types of branching: tip and lateral branching. Compared with the parent strain, lateral branching but not tip branching was drastically altered in the ΔerpΔpδ strain (Fig. 4a). The angles between the main hyphae and lateral branches in the parent and ΔerpΔpδ strains were statistically analysed (Fig. 4b). The main frequency distribution of the branching angles ranged from 70° to 90° in the parent strain and 10° to 30° in the ΔerpΔpδ strain. The branching angle in the parent strain was 77.78° ± 13.68° (mean ± s.d., n = 300), while in the ΔerpΔpδ strain it was 21.82° ± 11.89° (mean ± s.d., n = 300). These results suggest that the p24 heterodimer participates in lateral branching.


Function of a p24 Heterodimer in Morphogenesis and Protein Transport in Penicillium oxalicum.

Wang F, Liu K, Han L, Jiang B, Wang M, Fang X - Sci Rep (2015)

Comparison of Lateral Branching Development Patterns Between the Parent and ΔerpΔpδ Strains.Panel a, microscopic observation of hyphae from the parent and ΔerpΔpδ strains. Scale bar: 10 μm. Panel b, statistical comparison of angle distribution between main hyphae and lateral branches in the parent and ΔerpΔpδ strains. White represents the parent strain, and black represents the ΔerpΔpδ strain. Error bars indicate the standard deviation calculated from three biological experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493713&req=5

f4: Comparison of Lateral Branching Development Patterns Between the Parent and ΔerpΔpδ Strains.Panel a, microscopic observation of hyphae from the parent and ΔerpΔpδ strains. Scale bar: 10 μm. Panel b, statistical comparison of angle distribution between main hyphae and lateral branches in the parent and ΔerpΔpδ strains. White represents the parent strain, and black represents the ΔerpΔpδ strain. Error bars indicate the standard deviation calculated from three biological experiments.
Mentions: An increased branch frequency with dichotomous tips was observed for T. reesei Δerp but not P. oxalicum Δerp when the strains were cultivated on agar plates containing 2% glucose. This indicates that the branching mechanism was disrupted in T. reesei Δerp. To test whether branching was affected in the ΔerpΔpδ strain, approximately 1 × 103 spores were inoculated on glass slides covered by glucose-containing agar media. There are two types of branching: tip and lateral branching. Compared with the parent strain, lateral branching but not tip branching was drastically altered in the ΔerpΔpδ strain (Fig. 4a). The angles between the main hyphae and lateral branches in the parent and ΔerpΔpδ strains were statistically analysed (Fig. 4b). The main frequency distribution of the branching angles ranged from 70° to 90° in the parent strain and 10° to 30° in the ΔerpΔpδ strain. The branching angle in the parent strain was 77.78° ± 13.68° (mean ± s.d., n = 300), while in the ΔerpΔpδ strain it was 21.82° ± 11.89° (mean ± s.d., n = 300). These results suggest that the p24 heterodimer participates in lateral branching.

Bottom Line: The results suggested that the p24 heterodimer mediates protein transport, particularly that of cellobiohydrolase.These results suggest that the p24 heterodimer participates in morphogenesis and protein transport.Compared with P. oxalicum Δerp, a greater number of cellular physiological pathways were impaired in ΔerpΔpδ.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, Shandong, China.

ABSTRACT
The lignocellulose degradation capacity of filamentous fungi has been widely studied because of their cellulase hypersecretion. The p24 proteins in eukaryotes serve important functions in this secretory pathway. However, little is known about the functions of the p24 proteins in filamentous fungi. In this study, four p24 proteins were identified in Penicillium oxalicum. Six p24 double-deletion strains were constructed, and further studies were carried out with the ΔerpΔpδ strain. The experimental results suggested that Erp and Pδ form a p24 heterodimer in vivo. This p24 heterodimer participates in important morphogenetic events, including sporulation, hyphal growth, and lateral branching. The results suggested that the p24 heterodimer mediates protein transport, particularly that of cellobiohydrolase. Analysis of the intracellular proteome revealed that the ΔerpΔpδ double mutant is under secretion stress due to attempts to remove proteins that are jammed in the endomembrane system. These results suggest that the p24 heterodimer participates in morphogenesis and protein transport. Compared with P. oxalicum Δerp, a greater number of cellular physiological pathways were impaired in ΔerpΔpδ. This finding may provide new insights into the secretory pathways of filamentous fungi.

No MeSH data available.


Related in: MedlinePlus