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Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus.

Tan X, Qin N, Wu C, Sheng J, Yang R, Zheng B, Ma Z, Liu L, Peng X, Jia A - Sci Rep (2015)

Bottom Line: These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function.Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm.Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

ABSTRACT
Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain, and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

No MeSH data available.


Related in: MedlinePlus

Expression ratio (log2) obtained by qRT-PCR and RNA-seq of these six selected genes associated with S. aureus biofilm formation.pyk was used as a reference gene for normalization of qRT-PCR data. All gene expression ratios from qRT-PCR between treatment groups and controls under different conditions were significantly different (P < 0.05). Bars represent the error standard (n = 3). The x-axis indicates the comparison between treatment groups and controls under different conditions. The y-axis shows the gene expression ratios.
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f4: Expression ratio (log2) obtained by qRT-PCR and RNA-seq of these six selected genes associated with S. aureus biofilm formation.pyk was used as a reference gene for normalization of qRT-PCR data. All gene expression ratios from qRT-PCR between treatment groups and controls under different conditions were significantly different (P < 0.05). Bars represent the error standard (n = 3). The x-axis indicates the comparison between treatment groups and controls under different conditions. The y-axis shows the gene expression ratios.

Mentions: On the basis of these six genes (agrA, hld, icaR, spa, cna, and bbp) were significantly differential transcribed (P < 0.05, FDR < 0.001) under inhibiting biofilm formation condition or removing established biofilm condition and their expression was related to S. aureus biofilm formation, they were selected for qRT-PCR to investigate gene expression difference between treatment samples and controls. The results of gene expression ratios between treatment groups and controls are shown in Fig. 4. Although the trend of hld expression ratio under removing established biofilm condition was inconsistent between RNA-seq and qRT-PCR, a liner regression analysis showed an overall correlation of r = 0.842 for these six genes under inhibiting biofilm formation condition or removing established biofilm condition, which indicates a high correlation between transcript abundance assayed by qRT-PCR and the transcription profile revealed by RNA-seq data24. In general, although the exact fold difference for each gene by qRT-PCR was different from the RNA-seq, the comparison pairs had the similar trends with RNA-seq, which suggested that there would be the relative high consistency between RNA-seq and qRT-PCR.


Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus.

Tan X, Qin N, Wu C, Sheng J, Yang R, Zheng B, Ma Z, Liu L, Peng X, Jia A - Sci Rep (2015)

Expression ratio (log2) obtained by qRT-PCR and RNA-seq of these six selected genes associated with S. aureus biofilm formation.pyk was used as a reference gene for normalization of qRT-PCR data. All gene expression ratios from qRT-PCR between treatment groups and controls under different conditions were significantly different (P < 0.05). Bars represent the error standard (n = 3). The x-axis indicates the comparison between treatment groups and controls under different conditions. The y-axis shows the gene expression ratios.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493712&req=5

f4: Expression ratio (log2) obtained by qRT-PCR and RNA-seq of these six selected genes associated with S. aureus biofilm formation.pyk was used as a reference gene for normalization of qRT-PCR data. All gene expression ratios from qRT-PCR between treatment groups and controls under different conditions were significantly different (P < 0.05). Bars represent the error standard (n = 3). The x-axis indicates the comparison between treatment groups and controls under different conditions. The y-axis shows the gene expression ratios.
Mentions: On the basis of these six genes (agrA, hld, icaR, spa, cna, and bbp) were significantly differential transcribed (P < 0.05, FDR < 0.001) under inhibiting biofilm formation condition or removing established biofilm condition and their expression was related to S. aureus biofilm formation, they were selected for qRT-PCR to investigate gene expression difference between treatment samples and controls. The results of gene expression ratios between treatment groups and controls are shown in Fig. 4. Although the trend of hld expression ratio under removing established biofilm condition was inconsistent between RNA-seq and qRT-PCR, a liner regression analysis showed an overall correlation of r = 0.842 for these six genes under inhibiting biofilm formation condition or removing established biofilm condition, which indicates a high correlation between transcript abundance assayed by qRT-PCR and the transcription profile revealed by RNA-seq data24. In general, although the exact fold difference for each gene by qRT-PCR was different from the RNA-seq, the comparison pairs had the similar trends with RNA-seq, which suggested that there would be the relative high consistency between RNA-seq and qRT-PCR.

Bottom Line: These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function.Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm.Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

ABSTRACT
Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain, and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

No MeSH data available.


Related in: MedlinePlus