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Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus.

Tan X, Qin N, Wu C, Sheng J, Yang R, Zheng B, Ma Z, Liu L, Peng X, Jia A - Sci Rep (2015)

Bottom Line: These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function.Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm.Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

ABSTRACT
Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain, and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopic images showing the structure of the biofilm of Staphylococcus aureus ATCC25923.Magnifications, ×3000. (a) Control without ethanol (18-h incubation) (218), (b) 30 μg/mL ursolic acid (2U30), (c) 100 μg/mL resveratrol (2R100), (d) control without ethanol (36 h) (236), (e) 150 μg/mL resveratrol (2R150), (f) 8 μg/mL vancomycin (2V), and (g) the mixture of 8 μg/mL vancomycin and 150 μg/mL resveratrol.
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f1: Scanning electron microscopic images showing the structure of the biofilm of Staphylococcus aureus ATCC25923.Magnifications, ×3000. (a) Control without ethanol (18-h incubation) (218), (b) 30 μg/mL ursolic acid (2U30), (c) 100 μg/mL resveratrol (2R100), (d) control without ethanol (36 h) (236), (e) 150 μg/mL resveratrol (2R150), (f) 8 μg/mL vancomycin (2V), and (g) the mixture of 8 μg/mL vancomycin and 150 μg/mL resveratrol.

Mentions: The MSSA was able to form biofilms on 96-well plates after 18 h incubation. The SEM images showed that the MSSA formed thick, heterogenous clumps on the coverslips (Fig. 1a,d), which was thicker than MRSA biofilm in the same conditions18. Crystal violet staining assays revealed that ursolic acid could inhibit MSSA biofilm formation with an inhibitory rate of 46.50% (Supplementary Fig. S1 online). Moreover, the SEM image showed ursolic acid could inhibit the MSSA biofilm formation (Fig. 1b), while resveratrol could not inhibit the MSSA biofilm formation (Fig. 1c, Supplementary Fig. S1 online).


Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus.

Tan X, Qin N, Wu C, Sheng J, Yang R, Zheng B, Ma Z, Liu L, Peng X, Jia A - Sci Rep (2015)

Scanning electron microscopic images showing the structure of the biofilm of Staphylococcus aureus ATCC25923.Magnifications, ×3000. (a) Control without ethanol (18-h incubation) (218), (b) 30 μg/mL ursolic acid (2U30), (c) 100 μg/mL resveratrol (2R100), (d) control without ethanol (36 h) (236), (e) 150 μg/mL resveratrol (2R150), (f) 8 μg/mL vancomycin (2V), and (g) the mixture of 8 μg/mL vancomycin and 150 μg/mL resveratrol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493712&req=5

f1: Scanning electron microscopic images showing the structure of the biofilm of Staphylococcus aureus ATCC25923.Magnifications, ×3000. (a) Control without ethanol (18-h incubation) (218), (b) 30 μg/mL ursolic acid (2U30), (c) 100 μg/mL resveratrol (2R100), (d) control without ethanol (36 h) (236), (e) 150 μg/mL resveratrol (2R150), (f) 8 μg/mL vancomycin (2V), and (g) the mixture of 8 μg/mL vancomycin and 150 μg/mL resveratrol.
Mentions: The MSSA was able to form biofilms on 96-well plates after 18 h incubation. The SEM images showed that the MSSA formed thick, heterogenous clumps on the coverslips (Fig. 1a,d), which was thicker than MRSA biofilm in the same conditions18. Crystal violet staining assays revealed that ursolic acid could inhibit MSSA biofilm formation with an inhibitory rate of 46.50% (Supplementary Fig. S1 online). Moreover, the SEM image showed ursolic acid could inhibit the MSSA biofilm formation (Fig. 1b), while resveratrol could not inhibit the MSSA biofilm formation (Fig. 1c, Supplementary Fig. S1 online).

Bottom Line: These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function.Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm.Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

View Article: PubMed Central - PubMed

Affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

ABSTRACT
Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain, and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.

No MeSH data available.


Related in: MedlinePlus