Limits...
Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs.

Jing L, Hou Y, Wu H, Miao Y, Li X, Cao J, Brameld JM, Parr T, Zhao S - Sci Rep (2015)

Bottom Line: Among them, miR-136, miR-30e-5p, miR-1, miR-208b, miR-199a, miR-101 and miR-29c were up-regulated, while miR-215, miR-365-5p, miR-486, miR-1271, miR-145, miR-99b, miR-191 and miR-10b were down-regulated in low RFI pigs.We conclude that decreasing mitochondrial energy metabolism, possibly through AMPK - PGC-1A pathways, and increasing muscle growth, through IGF-1/2 and TGF-β signaling pathways, are potential strategies for the improvement of FE in pigs (and possibly other livestock).This study provides new insights into the molecular mechanisms that determine RFI and FE in pigs.

View Article: PubMed Central - PubMed

Affiliation: Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.

ABSTRACT
Feed efficiency (FE) can be measured by feed conversion ratio (FCR) or residual feed intake (RFI). In this study, we measured the FE related phenotypes of 236 castrated purebred Yorkshire boars, and selected 10 extreme individuals with high and low RFI for transcriptome analysis. We used RNA-seq analyses to determine the differential expression of genes and miRNAs in skeletal muscle. There were 99 differentially expressed genes identified (q ≤ 0.05). The down-regulated genes were mainly involved in mitochondrial energy metabolism, including FABP3, RCAN, PPARGC1 (PGC-1A), HK2 and PRKAG2. The up-regulated genes were mainly involved in skeletal muscle differentiation and proliferation, including IGF2, PDE7A, CEBPD, PIK3R1 and MYH6. Moreover, 15 differentially expressed miRNAs (/log2FC/ ≥ 1, total reads count ≥ 20, p ≤ 0.05) were identified. Among them, miR-136, miR-30e-5p, miR-1, miR-208b, miR-199a, miR-101 and miR-29c were up-regulated, while miR-215, miR-365-5p, miR-486, miR-1271, miR-145, miR-99b, miR-191 and miR-10b were down-regulated in low RFI pigs. We conclude that decreasing mitochondrial energy metabolism, possibly through AMPK - PGC-1A pathways, and increasing muscle growth, through IGF-1/2 and TGF-β signaling pathways, are potential strategies for the improvement of FE in pigs (and possibly other livestock). This study provides new insights into the molecular mechanisms that determine RFI and FE in pigs.

No MeSH data available.


Related in: MedlinePlus

The key network of genes and miRNAs found to be differentially expressed in skeletal muscle from low RFI compared with high RFI pigs.The network diagram was made using Cytoscape.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493709&req=5

f3: The key network of genes and miRNAs found to be differentially expressed in skeletal muscle from low RFI compared with high RFI pigs.The network diagram was made using Cytoscape.

Mentions: Cytoscape v3.0.1 was used to integrate a potential network of differentially expressed genes and miRNAs interacting in pig skeletal muscle that might lead to differences in RFI (Fig. 3). When looking into the differentially expressed genes, we found that most of those genes were involved in mitochondrial activity, glycolysis or myogenesis pathways and were actually connected directly or indirectly through just one or two genes. In this network, the mitochondrial activity was separated into 3 parts: the uncoupling reaction, the mitochondria respiratory control and the mitochondria transcriptional control.


Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs.

Jing L, Hou Y, Wu H, Miao Y, Li X, Cao J, Brameld JM, Parr T, Zhao S - Sci Rep (2015)

The key network of genes and miRNAs found to be differentially expressed in skeletal muscle from low RFI compared with high RFI pigs.The network diagram was made using Cytoscape.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493709&req=5

f3: The key network of genes and miRNAs found to be differentially expressed in skeletal muscle from low RFI compared with high RFI pigs.The network diagram was made using Cytoscape.
Mentions: Cytoscape v3.0.1 was used to integrate a potential network of differentially expressed genes and miRNAs interacting in pig skeletal muscle that might lead to differences in RFI (Fig. 3). When looking into the differentially expressed genes, we found that most of those genes were involved in mitochondrial activity, glycolysis or myogenesis pathways and were actually connected directly or indirectly through just one or two genes. In this network, the mitochondrial activity was separated into 3 parts: the uncoupling reaction, the mitochondria respiratory control and the mitochondria transcriptional control.

Bottom Line: Among them, miR-136, miR-30e-5p, miR-1, miR-208b, miR-199a, miR-101 and miR-29c were up-regulated, while miR-215, miR-365-5p, miR-486, miR-1271, miR-145, miR-99b, miR-191 and miR-10b were down-regulated in low RFI pigs.We conclude that decreasing mitochondrial energy metabolism, possibly through AMPK - PGC-1A pathways, and increasing muscle growth, through IGF-1/2 and TGF-β signaling pathways, are potential strategies for the improvement of FE in pigs (and possibly other livestock).This study provides new insights into the molecular mechanisms that determine RFI and FE in pigs.

View Article: PubMed Central - PubMed

Affiliation: Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.

ABSTRACT
Feed efficiency (FE) can be measured by feed conversion ratio (FCR) or residual feed intake (RFI). In this study, we measured the FE related phenotypes of 236 castrated purebred Yorkshire boars, and selected 10 extreme individuals with high and low RFI for transcriptome analysis. We used RNA-seq analyses to determine the differential expression of genes and miRNAs in skeletal muscle. There were 99 differentially expressed genes identified (q ≤ 0.05). The down-regulated genes were mainly involved in mitochondrial energy metabolism, including FABP3, RCAN, PPARGC1 (PGC-1A), HK2 and PRKAG2. The up-regulated genes were mainly involved in skeletal muscle differentiation and proliferation, including IGF2, PDE7A, CEBPD, PIK3R1 and MYH6. Moreover, 15 differentially expressed miRNAs (/log2FC/ ≥ 1, total reads count ≥ 20, p ≤ 0.05) were identified. Among them, miR-136, miR-30e-5p, miR-1, miR-208b, miR-199a, miR-101 and miR-29c were up-regulated, while miR-215, miR-365-5p, miR-486, miR-1271, miR-145, miR-99b, miR-191 and miR-10b were down-regulated in low RFI pigs. We conclude that decreasing mitochondrial energy metabolism, possibly through AMPK - PGC-1A pathways, and increasing muscle growth, through IGF-1/2 and TGF-β signaling pathways, are potential strategies for the improvement of FE in pigs (and possibly other livestock). This study provides new insights into the molecular mechanisms that determine RFI and FE in pigs.

No MeSH data available.


Related in: MedlinePlus