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In situ microscopic observation of chitin and fungal cells with chitinous cell walls in hydrothermal conditions.

Deguchi S, Tsujii K, Horikoshi K - Sci Rep (2015)

Bottom Line: Recent findings of intact chitin in fossil records suggest surprisingly high recalcitrance of this biopolymer during hydrothermal treatments.We also know in the experience of everyday life that mushroom, cells of which have chitinous cell walls, do not fall apart however long they are simmered.The results show very hot and compressed water is needed to make mushrooms mushy.

View Article: PubMed Central - PubMed

Affiliation: Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.

ABSTRACT
Recent findings of intact chitin in fossil records suggest surprisingly high recalcitrance of this biopolymer during hydrothermal treatments. We also know in the experience of everyday life that mushroom, cells of which have chitinous cell walls, do not fall apart however long they are simmered. We used in situ optical microscopy to examine chitin and fungal cells with chitinous cell walls during hydrothermal treatments, and obtained direct evidence that they remained undegraded at temperatures well over 200 °C. The results show very hot and compressed water is needed to make mushrooms mushy.

No MeSH data available.


Morphological change of F. velutipes cells in hydrothermal conditions.a) A series of in situ high-resolution optical microscopic images showing hyphae of F. velutipes between 100 °C and 388 °C and at a constant pressure of 25 MPa. Each images are 327 μm × 192 μm. A video clip showing the whole process is available in Movie S3. (b) Change of length (circle) and width (square) of hyphae of F. velutipes as a function of temperature.
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f4: Morphological change of F. velutipes cells in hydrothermal conditions.a) A series of in situ high-resolution optical microscopic images showing hyphae of F. velutipes between 100 °C and 388 °C and at a constant pressure of 25 MPa. Each images are 327 μm × 192 μm. A video clip showing the whole process is available in Movie S3. (b) Change of length (circle) and width (square) of hyphae of F. velutipes as a function of temperature.

Mentions: The large size of the hyphae allowed us to follow the cell morphology in more detail at higher temperatures (Fig. 4a). The hyphae remained essentially unchanged up to approximately 200 °C. Upon further heating, the hyphae underwent a highly anisotropic morphological change with temperature. It shrank dramatically along the long axis, whereas the width of the hyphae remained unchanged. The hyphae eventually disappeared completely between 380 °C and 390 °C.


In situ microscopic observation of chitin and fungal cells with chitinous cell walls in hydrothermal conditions.

Deguchi S, Tsujii K, Horikoshi K - Sci Rep (2015)

Morphological change of F. velutipes cells in hydrothermal conditions.a) A series of in situ high-resolution optical microscopic images showing hyphae of F. velutipes between 100 °C and 388 °C and at a constant pressure of 25 MPa. Each images are 327 μm × 192 μm. A video clip showing the whole process is available in Movie S3. (b) Change of length (circle) and width (square) of hyphae of F. velutipes as a function of temperature.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493705&req=5

f4: Morphological change of F. velutipes cells in hydrothermal conditions.a) A series of in situ high-resolution optical microscopic images showing hyphae of F. velutipes between 100 °C and 388 °C and at a constant pressure of 25 MPa. Each images are 327 μm × 192 μm. A video clip showing the whole process is available in Movie S3. (b) Change of length (circle) and width (square) of hyphae of F. velutipes as a function of temperature.
Mentions: The large size of the hyphae allowed us to follow the cell morphology in more detail at higher temperatures (Fig. 4a). The hyphae remained essentially unchanged up to approximately 200 °C. Upon further heating, the hyphae underwent a highly anisotropic morphological change with temperature. It shrank dramatically along the long axis, whereas the width of the hyphae remained unchanged. The hyphae eventually disappeared completely between 380 °C and 390 °C.

Bottom Line: Recent findings of intact chitin in fossil records suggest surprisingly high recalcitrance of this biopolymer during hydrothermal treatments.We also know in the experience of everyday life that mushroom, cells of which have chitinous cell walls, do not fall apart however long they are simmered.The results show very hot and compressed water is needed to make mushrooms mushy.

View Article: PubMed Central - PubMed

Affiliation: Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.

ABSTRACT
Recent findings of intact chitin in fossil records suggest surprisingly high recalcitrance of this biopolymer during hydrothermal treatments. We also know in the experience of everyday life that mushroom, cells of which have chitinous cell walls, do not fall apart however long they are simmered. We used in situ optical microscopy to examine chitin and fungal cells with chitinous cell walls during hydrothermal treatments, and obtained direct evidence that they remained undegraded at temperatures well over 200 °C. The results show very hot and compressed water is needed to make mushrooms mushy.

No MeSH data available.