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Protein alterations in women with chronic widespread pain--An explorative proteomic study of the trapezius muscle.

Olausson P, Gerdle B, Ghafouri N, Sjöström D, Blixt E, Ghafouri B - Sci Rep (2015)

Bottom Line: Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects.Other proteins are associated with muscle damage, muscle recovery, stress and inflammation.Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

View Article: PubMed Central - PubMed

Affiliation: Division of Community Medicine, Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University and Pain and Rehabilitation Center, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland.

ABSTRACT
Chronic widespread pain (CWP) has a high prevalence in the population and is associated with prominent negative individual and societal consequences. There is no clear consensus concerning the etiology behind CWP although alterations in the central processing of nociception maintained by peripheral nociceptive input has been suggested. Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects. The 2-dimensional gel electrophoresis (2-DE) in combination with multivariate statistical analyses revealed 17 proteins to be differently expressed between the two groups. Proteins were identified by mass spectrometry. Many of the proteins are important enzymes in metabolic pathways like the glycolysis and gluconeogenesis. Other proteins are associated with muscle damage, muscle recovery, stress and inflammation. The altered expressed levels of these proteins suggest abnormalities and metabolic changes in the myalgic trapezius muscle in CWP. Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

No MeSH data available.


Related in: MedlinePlus

A schematic representing the metabolic pathways of the glycolysis and gluconeogenesis.The squared enzymatic proteins were all up-regulated in the CWP group compared to the healthy subjects except creatine kinase which was down-regulated. This could be explained by a higher dependence and therefore a higher strain on the glycolysis to provide energy for the skeletal muscle resulting in an accumulation of Pi, H+, pyruvate and lactate.
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f3: A schematic representing the metabolic pathways of the glycolysis and gluconeogenesis.The squared enzymatic proteins were all up-regulated in the CWP group compared to the healthy subjects except creatine kinase which was down-regulated. This could be explained by a higher dependence and therefore a higher strain on the glycolysis to provide energy for the skeletal muscle resulting in an accumulation of Pi, H+, pyruvate and lactate.

Mentions: Creatine kinase isoenzymes play a central role in energy transduction in tissues with large energy demands such as skeletal muscle. The creatine kinases catalyzes the transfer of phosphate between ATP and phosphocreatine thus buffering cellular ATP and ADP concentrations57 (Fig. 3). This reaction protects the muscle from entering into rigor by maintaining the ATP at the expense of phosphocreatine. Our results of decreased levels of creatine kinase B-type could result in not only lower levels of ATP, caused of the insufficient regeneration of ATP through the creatine kinase reaction, but also indirectly keeping a low muscle pH. When ADP together with Pi is converted into ATP a H+ is also consumed in the creation of creatine (Fig. 3). Considering the biochemistry, with a low cytoplasmic ATP, the muscle would end up in a “rigor-like” state i.e. a feeling of muscle stiffness which is one of the clinical symptoms of CWP5. Low ATP and low phosphocreatine has been detected in the quadriceps muscle of FMS and in the trapezius muscle of patients with chronic trapezius myalgia7778.


Protein alterations in women with chronic widespread pain--An explorative proteomic study of the trapezius muscle.

Olausson P, Gerdle B, Ghafouri N, Sjöström D, Blixt E, Ghafouri B - Sci Rep (2015)

A schematic representing the metabolic pathways of the glycolysis and gluconeogenesis.The squared enzymatic proteins were all up-regulated in the CWP group compared to the healthy subjects except creatine kinase which was down-regulated. This could be explained by a higher dependence and therefore a higher strain on the glycolysis to provide energy for the skeletal muscle resulting in an accumulation of Pi, H+, pyruvate and lactate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493691&req=5

f3: A schematic representing the metabolic pathways of the glycolysis and gluconeogenesis.The squared enzymatic proteins were all up-regulated in the CWP group compared to the healthy subjects except creatine kinase which was down-regulated. This could be explained by a higher dependence and therefore a higher strain on the glycolysis to provide energy for the skeletal muscle resulting in an accumulation of Pi, H+, pyruvate and lactate.
Mentions: Creatine kinase isoenzymes play a central role in energy transduction in tissues with large energy demands such as skeletal muscle. The creatine kinases catalyzes the transfer of phosphate between ATP and phosphocreatine thus buffering cellular ATP and ADP concentrations57 (Fig. 3). This reaction protects the muscle from entering into rigor by maintaining the ATP at the expense of phosphocreatine. Our results of decreased levels of creatine kinase B-type could result in not only lower levels of ATP, caused of the insufficient regeneration of ATP through the creatine kinase reaction, but also indirectly keeping a low muscle pH. When ADP together with Pi is converted into ATP a H+ is also consumed in the creation of creatine (Fig. 3). Considering the biochemistry, with a low cytoplasmic ATP, the muscle would end up in a “rigor-like” state i.e. a feeling of muscle stiffness which is one of the clinical symptoms of CWP5. Low ATP and low phosphocreatine has been detected in the quadriceps muscle of FMS and in the trapezius muscle of patients with chronic trapezius myalgia7778.

Bottom Line: Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects.Other proteins are associated with muscle damage, muscle recovery, stress and inflammation.Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

View Article: PubMed Central - PubMed

Affiliation: Division of Community Medicine, Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University and Pain and Rehabilitation Center, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland.

ABSTRACT
Chronic widespread pain (CWP) has a high prevalence in the population and is associated with prominent negative individual and societal consequences. There is no clear consensus concerning the etiology behind CWP although alterations in the central processing of nociception maintained by peripheral nociceptive input has been suggested. Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects. The 2-dimensional gel electrophoresis (2-DE) in combination with multivariate statistical analyses revealed 17 proteins to be differently expressed between the two groups. Proteins were identified by mass spectrometry. Many of the proteins are important enzymes in metabolic pathways like the glycolysis and gluconeogenesis. Other proteins are associated with muscle damage, muscle recovery, stress and inflammation. The altered expressed levels of these proteins suggest abnormalities and metabolic changes in the myalgic trapezius muscle in CWP. Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

No MeSH data available.


Related in: MedlinePlus