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Protein alterations in women with chronic widespread pain--An explorative proteomic study of the trapezius muscle.

Olausson P, Gerdle B, Ghafouri N, Sjöström D, Blixt E, Ghafouri B - Sci Rep (2015)

Bottom Line: Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects.Other proteins are associated with muscle damage, muscle recovery, stress and inflammation.Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

View Article: PubMed Central - PubMed

Affiliation: Division of Community Medicine, Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University and Pain and Rehabilitation Center, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland.

ABSTRACT
Chronic widespread pain (CWP) has a high prevalence in the population and is associated with prominent negative individual and societal consequences. There is no clear consensus concerning the etiology behind CWP although alterations in the central processing of nociception maintained by peripheral nociceptive input has been suggested. Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects. The 2-dimensional gel electrophoresis (2-DE) in combination with multivariate statistical analyses revealed 17 proteins to be differently expressed between the two groups. Proteins were identified by mass spectrometry. Many of the proteins are important enzymes in metabolic pathways like the glycolysis and gluconeogenesis. Other proteins are associated with muscle damage, muscle recovery, stress and inflammation. The altered expressed levels of these proteins suggest abnormalities and metabolic changes in the myalgic trapezius muscle in CWP. Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

No MeSH data available.


Related in: MedlinePlus

An analytical 2-DE pattern from human trapezius muscle.Numbers represent the identified proteins significantly altered according to the univariate and multivariate analysis, i.e. between groups (CWP vs CON). Horizontally the proteins are separated according to isoelectric point (pI) and vertically based on their molecular weight (MW).
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f1: An analytical 2-DE pattern from human trapezius muscle.Numbers represent the identified proteins significantly altered according to the univariate and multivariate analysis, i.e. between groups (CWP vs CON). Horizontally the proteins are separated according to isoelectric point (pI) and vertically based on their molecular weight (MW).

Mentions: Seventeen proteins were significantly altered between the groups (p < 0.05) according to the traditional statistical analyses and were selected for identification and in-gel digested for nLC/MS/MS or MALDI-TOF. Twelve of these were up-regulated (↑) in the CWP group and five were down-regulated (↓) (Fig. 1, Table 2 and Supplementary Appendix A).


Protein alterations in women with chronic widespread pain--An explorative proteomic study of the trapezius muscle.

Olausson P, Gerdle B, Ghafouri N, Sjöström D, Blixt E, Ghafouri B - Sci Rep (2015)

An analytical 2-DE pattern from human trapezius muscle.Numbers represent the identified proteins significantly altered according to the univariate and multivariate analysis, i.e. between groups (CWP vs CON). Horizontally the proteins are separated according to isoelectric point (pI) and vertically based on their molecular weight (MW).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493691&req=5

f1: An analytical 2-DE pattern from human trapezius muscle.Numbers represent the identified proteins significantly altered according to the univariate and multivariate analysis, i.e. between groups (CWP vs CON). Horizontally the proteins are separated according to isoelectric point (pI) and vertically based on their molecular weight (MW).
Mentions: Seventeen proteins were significantly altered between the groups (p < 0.05) according to the traditional statistical analyses and were selected for identification and in-gel digested for nLC/MS/MS or MALDI-TOF. Twelve of these were up-regulated (↑) in the CWP group and five were down-regulated (↓) (Fig. 1, Table 2 and Supplementary Appendix A).

Bottom Line: Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects.Other proteins are associated with muscle damage, muscle recovery, stress and inflammation.Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

View Article: PubMed Central - PubMed

Affiliation: Division of Community Medicine, Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University and Pain and Rehabilitation Center, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland.

ABSTRACT
Chronic widespread pain (CWP) has a high prevalence in the population and is associated with prominent negative individual and societal consequences. There is no clear consensus concerning the etiology behind CWP although alterations in the central processing of nociception maintained by peripheral nociceptive input has been suggested. Here, we use proteomics to study protein changes in trapezius muscle from 18 female patients diagnosed with CWP compared to 19 healthy female subjects. The 2-dimensional gel electrophoresis (2-DE) in combination with multivariate statistical analyses revealed 17 proteins to be differently expressed between the two groups. Proteins were identified by mass spectrometry. Many of the proteins are important enzymes in metabolic pathways like the glycolysis and gluconeogenesis. Other proteins are associated with muscle damage, muscle recovery, stress and inflammation. The altered expressed levels of these proteins suggest abnormalities and metabolic changes in the myalgic trapezius muscle in CWP. Taken together, this study gives further support that peripheral factors may be of importance in maintaining CWP.

No MeSH data available.


Related in: MedlinePlus