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Imaging neuroinflammation? A perspective from MR spectroscopy.

Zahr NM, Mayer D, Rohlfing T, Sullivan EV, Pfefferbaum A - Brain Pathol. (2014)

Bottom Line: Significant four-group comparisons were evident only for striatal choline-containing compounds (Cho) and myo-inositol (mI), which follow-up analysis demonstrated were due to higher levels in HA compared with C individuals.Higher levels of mI were related to greater lifetime alcohol consumed, whereas HAART was associated with lower mI levels.The current results suggest that competing mechanisms can influence in vivo Cho and mI levels, and that elevations in these metabolites cannot necessarily be interpreted as reflecting a single underlying mechanism, including neuroinflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine (MC5723), Stanford, CA; Neuroscience Program, SRI International, Menlo Park, CA.

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Related in: MedlinePlus

(A) Voxel placement in striatum, cerebellum and pons, and (B) spectra averaged from control subjects in the striatal voxel. a.u. = arbitrary units; Cho = choline-containing compounds; Glu = glutamate; mI = myo-inositol; NAA = N-acetyl aspartate; tCr = total creatine.
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fig01: (A) Voxel placement in striatum, cerebellum and pons, and (B) spectra averaged from control subjects in the striatal voxel. a.u. = arbitrary units; Cho = choline-containing compounds; Glu = glutamate; mI = myo-inositol; NAA = N-acetyl aspartate; tCr = total creatine.

Mentions: MRS was performed using constant time point-resolved spectroscopy (CT-PRESS) (30). Single voxels were manually positioned in left or right striatum (10.6 cc), left or right cerebellum (9.8 cc) and central pons (5.9 cc); hemisphere of voxel placement was balanced across subjects and groups (Figure 1A). The acquisition time was ∼9 minute per voxel [TE = 139 ms, 129 chemical shift (CS) encoding steps, Δt1/2 = 0.8 ms, TR = 2 s, 2 averages] (66). A scan without water suppression was acquired (17 CS encoding steps, Δt1/2 = 6.4 ms, 2 averages) to measure the tissue water content used to normalize the metabolite signal intensities. Data acquired without water suppression were apodized in t2 with a 5 Hz Gaussian line broadening and zero filled up to 4K points for each TE.


Imaging neuroinflammation? A perspective from MR spectroscopy.

Zahr NM, Mayer D, Rohlfing T, Sullivan EV, Pfefferbaum A - Brain Pathol. (2014)

(A) Voxel placement in striatum, cerebellum and pons, and (B) spectra averaged from control subjects in the striatal voxel. a.u. = arbitrary units; Cho = choline-containing compounds; Glu = glutamate; mI = myo-inositol; NAA = N-acetyl aspartate; tCr = total creatine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493672&req=5

fig01: (A) Voxel placement in striatum, cerebellum and pons, and (B) spectra averaged from control subjects in the striatal voxel. a.u. = arbitrary units; Cho = choline-containing compounds; Glu = glutamate; mI = myo-inositol; NAA = N-acetyl aspartate; tCr = total creatine.
Mentions: MRS was performed using constant time point-resolved spectroscopy (CT-PRESS) (30). Single voxels were manually positioned in left or right striatum (10.6 cc), left or right cerebellum (9.8 cc) and central pons (5.9 cc); hemisphere of voxel placement was balanced across subjects and groups (Figure 1A). The acquisition time was ∼9 minute per voxel [TE = 139 ms, 129 chemical shift (CS) encoding steps, Δt1/2 = 0.8 ms, TR = 2 s, 2 averages] (66). A scan without water suppression was acquired (17 CS encoding steps, Δt1/2 = 6.4 ms, 2 averages) to measure the tissue water content used to normalize the metabolite signal intensities. Data acquired without water suppression were apodized in t2 with a 5 Hz Gaussian line broadening and zero filled up to 4K points for each TE.

Bottom Line: Significant four-group comparisons were evident only for striatal choline-containing compounds (Cho) and myo-inositol (mI), which follow-up analysis demonstrated were due to higher levels in HA compared with C individuals.Higher levels of mI were related to greater lifetime alcohol consumed, whereas HAART was associated with lower mI levels.The current results suggest that competing mechanisms can influence in vivo Cho and mI levels, and that elevations in these metabolites cannot necessarily be interpreted as reflecting a single underlying mechanism, including neuroinflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine (MC5723), Stanford, CA; Neuroscience Program, SRI International, Menlo Park, CA.

Show MeSH
Related in: MedlinePlus