Limits...
Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae).

Frías-López C, Almeida FC, Guirao-Rico S, Vizueta J, Sánchez-Gracia A, Arnedo MA, Rozas J - PeerJ (2015)

Bottom Line: We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes.Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs.Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona , Barcelona , Spain ; Departament de Biologia Animal and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona , Barcelona , Spain.

ABSTRACT
The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limitation, we launched the first tissue-specific enriched RNA-seq analysis in this species using a subtractive hybridization approach, with two main objectives, to characterize the specific transcriptome of the putative chemosensory appendages (palps and first pair of legs), and to provide a new set of DNA markers for further phylogenetic studies. We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes. Among specific candidates, we have identified some members of the iGluR and NPC2 families. Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs. Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic relationships of major Mygalomorphae lineages sampled.ML tree showing the phylogenetic relationships of major Mygalomorphae lineages sampled. The analysis is based on a supermatrix of 35 putative orthologs (4,531 amino acids). Numbers indicate bootstrap support values >50%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493671&req=5

fig-6: Phylogenetic relationships of major Mygalomorphae lineages sampled.ML tree showing the phylogenetic relationships of major Mygalomorphae lineages sampled. The analysis is based on a supermatrix of 35 putative orthologs (4,531 amino acids). Numbers indicate bootstrap support values >50%.

Mentions: From the data matrix d327 of Bond et al. (2014), we built a new MSA with information of M. calpeiana obtained from our transcriptome analysis. We have filtered the data in order to include high quality homologous data with high coverage per taxon. Our final MSA comprises 17 Mygalomorph species (including M. calpeiana) and 3 non-mygalomorph outgroups (20 taxa; 35 genes; 4,531 amino acids; Table S8), with an average taxa coverage of 17.1. Our ML phylogenetic tree, rooted using Liphistus as an outgroup, mirrors those reported in Bond et al. (2014) and shows M. calpeiana as the sister lineage of the genus Paratropis (Fig. 6), albeit with low node support (57%), as part of the non-Bipectina Avicularioidea. Interestingly, in a recent study focused on the phylogenetic relationship and biogeographic origins of the genus Macrothele (Opatova & Arnedo, 2014) based on a denser taxonomic sampling but lower gene coverage (3 genes), a similar position of Macrothele, within the Aviculariodea but outside the Bipectina lineage, was also recovered.


Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae).

Frías-López C, Almeida FC, Guirao-Rico S, Vizueta J, Sánchez-Gracia A, Arnedo MA, Rozas J - PeerJ (2015)

Phylogenetic relationships of major Mygalomorphae lineages sampled.ML tree showing the phylogenetic relationships of major Mygalomorphae lineages sampled. The analysis is based on a supermatrix of 35 putative orthologs (4,531 amino acids). Numbers indicate bootstrap support values >50%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493671&req=5

fig-6: Phylogenetic relationships of major Mygalomorphae lineages sampled.ML tree showing the phylogenetic relationships of major Mygalomorphae lineages sampled. The analysis is based on a supermatrix of 35 putative orthologs (4,531 amino acids). Numbers indicate bootstrap support values >50%.
Mentions: From the data matrix d327 of Bond et al. (2014), we built a new MSA with information of M. calpeiana obtained from our transcriptome analysis. We have filtered the data in order to include high quality homologous data with high coverage per taxon. Our final MSA comprises 17 Mygalomorph species (including M. calpeiana) and 3 non-mygalomorph outgroups (20 taxa; 35 genes; 4,531 amino acids; Table S8), with an average taxa coverage of 17.1. Our ML phylogenetic tree, rooted using Liphistus as an outgroup, mirrors those reported in Bond et al. (2014) and shows M. calpeiana as the sister lineage of the genus Paratropis (Fig. 6), albeit with low node support (57%), as part of the non-Bipectina Avicularioidea. Interestingly, in a recent study focused on the phylogenetic relationship and biogeographic origins of the genus Macrothele (Opatova & Arnedo, 2014) based on a denser taxonomic sampling but lower gene coverage (3 genes), a similar position of Macrothele, within the Aviculariodea but outside the Bipectina lineage, was also recovered.

Bottom Line: We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes.Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs.Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona , Barcelona , Spain ; Departament de Biologia Animal and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona , Barcelona , Spain.

ABSTRACT
The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limitation, we launched the first tissue-specific enriched RNA-seq analysis in this species using a subtractive hybridization approach, with two main objectives, to characterize the specific transcriptome of the putative chemosensory appendages (palps and first pair of legs), and to provide a new set of DNA markers for further phylogenetic studies. We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes. Among specific candidates, we have identified some members of the iGluR and NPC2 families. Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs. Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance.

No MeSH data available.


Related in: MedlinePlus