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A High Throughput Micro-Chamber Array Device for Single Cell Clonal Cultivation and Tumor Heterogeneity Analysis.

Shen FM, Zhu L, Ye H, Yang YJ, Pang DW, Zhang ZL - Sci Rep (2015)

Bottom Line: Recently, single cell cloning techniques have been gradually developed benefited from their important roles in monoclonal antibody screening, tumor heterogeneity research fields, etc.In this study, we developed a high throughput device containing 1400 lateral chambers to efficiently isolate single cells and carry out long-term single cell clonal cultivation as well as tumor heterogeneity studies.The device was further used to examine tumor heterogeneity such as morphology, growth rate, anti-cancer drug tolerance as well as adenosine triphosphate-binding cassette (ABC) transporter ABCG2 protein expression level.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China.

ABSTRACT
Recently, single cell cloning techniques have been gradually developed benefited from their important roles in monoclonal antibody screening, tumor heterogeneity research fields, etc. In this study, we developed a high throughput device containing 1400 lateral chambers to efficiently isolate single cells and carry out long-term single cell clonal cultivation as well as tumor heterogeneity studies. Most of the isolated single cells could proliferate normally nearly as long as three weeks and hundreds of clones could be formed once with one device, which made it possible to study tumor heterogeneity at single cell level. The device was further used to examine tumor heterogeneity such as morphology, growth rate, anti-cancer drug tolerance as well as adenosine triphosphate-binding cassette (ABC) transporter ABCG2 protein expression level. Except for the single cell isolation and tumor heterogeneity studies, the device is expected to be used as an excellent platform for drug screening, tumor biomarker discovering and tumor metastasis assay.

No MeSH data available.


Related in: MedlinePlus

Growth curves of both type 1 and type 2 MHCC 97L cells in chip chambers.
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Related In: Results  -  Collection

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f5: Growth curves of both type 1 and type 2 MHCC 97L cells in chip chambers.

Mentions: Some reports pointed out that growth rate, sensitivity to drug and other characterizations may be heterogeneous under different cell morphologies3435. Here, the device was also used to study tumor heterogeneity, the differences on growth rate, sensitivity to indomethacin and ABCG2 protein expression level between the two types of MHCC 97L clones were further examined in this work. Firstly, we investigated the growth rate of the two kinds of clones. According to the references3637, the cell covering area was used to calculate cell growth rate in consideration of the irregular morphologies. Type 1 MHCC 97L cells grew adherently as multilayer, we took the maximum coverage area of the type 1 MHCC 97L cells into account as the final area. The growth curves of the two clones seemed similar as shown in Fig. 5.


A High Throughput Micro-Chamber Array Device for Single Cell Clonal Cultivation and Tumor Heterogeneity Analysis.

Shen FM, Zhu L, Ye H, Yang YJ, Pang DW, Zhang ZL - Sci Rep (2015)

Growth curves of both type 1 and type 2 MHCC 97L cells in chip chambers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493670&req=5

f5: Growth curves of both type 1 and type 2 MHCC 97L cells in chip chambers.
Mentions: Some reports pointed out that growth rate, sensitivity to drug and other characterizations may be heterogeneous under different cell morphologies3435. Here, the device was also used to study tumor heterogeneity, the differences on growth rate, sensitivity to indomethacin and ABCG2 protein expression level between the two types of MHCC 97L clones were further examined in this work. Firstly, we investigated the growth rate of the two kinds of clones. According to the references3637, the cell covering area was used to calculate cell growth rate in consideration of the irregular morphologies. Type 1 MHCC 97L cells grew adherently as multilayer, we took the maximum coverage area of the type 1 MHCC 97L cells into account as the final area. The growth curves of the two clones seemed similar as shown in Fig. 5.

Bottom Line: Recently, single cell cloning techniques have been gradually developed benefited from their important roles in monoclonal antibody screening, tumor heterogeneity research fields, etc.In this study, we developed a high throughput device containing 1400 lateral chambers to efficiently isolate single cells and carry out long-term single cell clonal cultivation as well as tumor heterogeneity studies.The device was further used to examine tumor heterogeneity such as morphology, growth rate, anti-cancer drug tolerance as well as adenosine triphosphate-binding cassette (ABC) transporter ABCG2 protein expression level.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China.

ABSTRACT
Recently, single cell cloning techniques have been gradually developed benefited from their important roles in monoclonal antibody screening, tumor heterogeneity research fields, etc. In this study, we developed a high throughput device containing 1400 lateral chambers to efficiently isolate single cells and carry out long-term single cell clonal cultivation as well as tumor heterogeneity studies. Most of the isolated single cells could proliferate normally nearly as long as three weeks and hundreds of clones could be formed once with one device, which made it possible to study tumor heterogeneity at single cell level. The device was further used to examine tumor heterogeneity such as morphology, growth rate, anti-cancer drug tolerance as well as adenosine triphosphate-binding cassette (ABC) transporter ABCG2 protein expression level. Except for the single cell isolation and tumor heterogeneity studies, the device is expected to be used as an excellent platform for drug screening, tumor biomarker discovering and tumor metastasis assay.

No MeSH data available.


Related in: MedlinePlus