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Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus

Phenotypic Properties for Pre-induced Lac Operon on 5 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.
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Figure 5: Phenotypic Properties for Pre-induced Lac Operon on 5 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.

Mentions: At 5mM of lactose concentration in the EEP the growth rate followed an increasing trend till 25 µM of IPTG concentration and thereafter it saturates (Fig. 5a). In LEP the growth phenotype was not observed as in the EEP. At 5 mM of lactose concentration in LEP the growth rate was slightly higher for 0 and 1 µM concentrations of IPTG than 25 and 200 µM of IPTG used in the pre-culture (Fig. 5b). In the EEP β-galactosidase measurement showed increasing trend till 25 µM of IPTG concentration after which it saturates (Fig. 5c). In the LEP the β-galactosidase measurement was almost similar for all cells irrespective of whether they are pre-induced or not (Fig. 5d). These results pointed towards the fact that strength of substrate plays an important role in influencing the effect of pre-induced lac operon on lactose environment. This effect also depends on the growth stage i.e. whether the cells are in EEP or in LEP.


Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Phenotypic Properties for Pre-induced Lac Operon on 5 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493661&req=5

Figure 5: Phenotypic Properties for Pre-induced Lac Operon on 5 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.
Mentions: At 5mM of lactose concentration in the EEP the growth rate followed an increasing trend till 25 µM of IPTG concentration and thereafter it saturates (Fig. 5a). In LEP the growth phenotype was not observed as in the EEP. At 5 mM of lactose concentration in LEP the growth rate was slightly higher for 0 and 1 µM concentrations of IPTG than 25 and 200 µM of IPTG used in the pre-culture (Fig. 5b). In the EEP β-galactosidase measurement showed increasing trend till 25 µM of IPTG concentration after which it saturates (Fig. 5c). In the LEP the β-galactosidase measurement was almost similar for all cells irrespective of whether they are pre-induced or not (Fig. 5d). These results pointed towards the fact that strength of substrate plays an important role in influencing the effect of pre-induced lac operon on lactose environment. This effect also depends on the growth stage i.e. whether the cells are in EEP or in LEP.

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus