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Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Bottom Line: Pre-induced lac operon provides benefit on lactose environment.Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Phenotypic Properties for Pre-induced Lac Operon on 1 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.
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Figure 4: Phenotypic Properties for Pre-induced Lac Operon on 1 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.

Mentions: In the EEP the growth rate showed increasing trend with increasing strength of IPTG used in the pre-culture on 1 mM of lactose (Fig. 4a). The growth rate for the cells which are pre-induced with lac operon was more on lactose environment as compared to the ones which are not pre-induced. During LEP the growth rate of the cells which are pre-induced with IPTG was more than the cells which are not pre-induced or induced with very low concentration of IPTG (Fig. 4b).


Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Phenotypic Properties for Pre-induced Lac Operon on 1 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493661&req=5

Figure 4: Phenotypic Properties for Pre-induced Lac Operon on 1 mM of Lactose. (a) & (b) Growth rate for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in entry into Early exponential Phase and Late Exponential Phase. (c) & (d) β-galactosidase activity for cells exposed to different concentrations of IPTG in pre-culture on lactose medium in EEP and LEP. Concentrations of IPTG on X-axis represent the concentration of IPTG to which cells are exposed in pre-culture. Glycerol (1g/L) was used in pre-culture.
Mentions: In the EEP the growth rate showed increasing trend with increasing strength of IPTG used in the pre-culture on 1 mM of lactose (Fig. 4a). The growth rate for the cells which are pre-induced with lac operon was more on lactose environment as compared to the ones which are not pre-induced. During LEP the growth rate of the cells which are pre-induced with IPTG was more than the cells which are not pre-induced or induced with very low concentration of IPTG (Fig. 4b).

Bottom Line: Pre-induced lac operon provides benefit on lactose environment.Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.