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Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus

Phenotypic Growth Properties on Lactose: (a) Growth rate on lactose medium for cells exposed to either galactose or glucose as carbon source in pre-culture. (b) Percentage relative reduction in growth rate for cells which are exposed to glucose in pre-culture as compared to the ones which are exposed to galactose in pre-culture on lactcose medium. (c) β-galactosidase activity for cells. The glucose and galactose written on X-axis represents the carbon source in pre-culture. The concentration of lactose used is 5 mM.
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Figure 3: Phenotypic Growth Properties on Lactose: (a) Growth rate on lactose medium for cells exposed to either galactose or glucose as carbon source in pre-culture. (b) Percentage relative reduction in growth rate for cells which are exposed to glucose in pre-culture as compared to the ones which are exposed to galactose in pre-culture on lactcose medium. (c) β-galactosidase activity for cells. The glucose and galactose written on X-axis represents the carbon source in pre-culture. The concentration of lactose used is 5 mM.

Mentions: The study further investigated the possibility of the counter effect. For this the cells were grown on both glucose and galactose medium separately in pre-culture and then transferred on lactose medium. The cells which were grown on galactose medium as pre-culture showed higher growth rate on lactose environment as compared to the ones which were grown on glucose as pre-culture (Fig. 3a). The relative reduction in growth rate in percentage was found to be around 14% for the pre-exposed glucose cells (Fig. 3b). The β-galactosidase measurement did not differ much for the glucose or galactose pre-grown cells on lactose medium (Fig. 3c).


Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Phenotypic Growth Properties on Lactose: (a) Growth rate on lactose medium for cells exposed to either galactose or glucose as carbon source in pre-culture. (b) Percentage relative reduction in growth rate for cells which are exposed to glucose in pre-culture as compared to the ones which are exposed to galactose in pre-culture on lactcose medium. (c) β-galactosidase activity for cells. The glucose and galactose written on X-axis represents the carbon source in pre-culture. The concentration of lactose used is 5 mM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493661&req=5

Figure 3: Phenotypic Growth Properties on Lactose: (a) Growth rate on lactose medium for cells exposed to either galactose or glucose as carbon source in pre-culture. (b) Percentage relative reduction in growth rate for cells which are exposed to glucose in pre-culture as compared to the ones which are exposed to galactose in pre-culture on lactcose medium. (c) β-galactosidase activity for cells. The glucose and galactose written on X-axis represents the carbon source in pre-culture. The concentration of lactose used is 5 mM.
Mentions: The study further investigated the possibility of the counter effect. For this the cells were grown on both glucose and galactose medium separately in pre-culture and then transferred on lactose medium. The cells which were grown on galactose medium as pre-culture showed higher growth rate on lactose environment as compared to the ones which were grown on glucose as pre-culture (Fig. 3a). The relative reduction in growth rate in percentage was found to be around 14% for the pre-exposed glucose cells (Fig. 3b). The β-galactosidase measurement did not differ much for the glucose or galactose pre-grown cells on lactose medium (Fig. 3c).

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus