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Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus

Phenotypic Growth Properties on Glucose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Dashed Line with diamond represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Solid Line with square represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Glucose (4g/L) was used in experiment.
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Figure 2: Phenotypic Growth Properties on Glucose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Dashed Line with diamond represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Solid Line with square represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Glucose (4g/L) was used in experiment.

Mentions: In order to check whether pre-culture on 1g/L glycerol environment along with 200 µM IPTG will provide any benefit for other carbon sources over galactose and lactose. The effect of pre-culture with IPTG on glucose environment was investigated. For this cells were exposed to IPTG on glycerol environment and then grown on glucose environment. Growth kinetics of cells which are pre-induced for lac operon were similar to the ones which are not pre-induced (Fig. 2a). The percentage relative decrease in growth rate of the cells which are not exposed to IPTG was very small. It was around 2.5% (Fig. 2b). The biomass was also almost similar. The growth curve for cells which are pre-induced for lac operon did not show much difference as compared to the cells which are not induced (Fig. 2d). In order to confirm that the phenotypic observation is due to the pre-induced lac operon, the cells were subjected for β-galactosidase estimation. The cells with pre-induced lac operon showed β-galactosidase activity while the ones that were not induced showed no activity (Fig. 2c).


Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Phenotypic Growth Properties on Glucose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Dashed Line with diamond represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Solid Line with square represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Glucose (4g/L) was used in experiment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493661&req=5

Figure 2: Phenotypic Growth Properties on Glucose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Dashed Line with diamond represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Solid Line with square represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Glucose (4g/L) was used in experiment.
Mentions: In order to check whether pre-culture on 1g/L glycerol environment along with 200 µM IPTG will provide any benefit for other carbon sources over galactose and lactose. The effect of pre-culture with IPTG on glucose environment was investigated. For this cells were exposed to IPTG on glycerol environment and then grown on glucose environment. Growth kinetics of cells which are pre-induced for lac operon were similar to the ones which are not pre-induced (Fig. 2a). The percentage relative decrease in growth rate of the cells which are not exposed to IPTG was very small. It was around 2.5% (Fig. 2b). The biomass was also almost similar. The growth curve for cells which are pre-induced for lac operon did not show much difference as compared to the cells which are not induced (Fig. 2d). In order to confirm that the phenotypic observation is due to the pre-induced lac operon, the cells were subjected for β-galactosidase estimation. The cells with pre-induced lac operon showed β-galactosidase activity while the ones that were not induced showed no activity (Fig. 2c).

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus