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Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus

Phenotypic Growth Properties on Galactose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Solid Line with square represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Dashed Line with diamond represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Galactose (10g/L) was used in experiment.
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Figure 1: Phenotypic Growth Properties on Galactose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Solid Line with square represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Dashed Line with diamond represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Galactose (10g/L) was used in experiment.

Mentions: The focus of the study was to characterize the effect of pre-induced lac operon on glucose and galactose milieu along with the factors that influence the effect of pre-induced lac operon. Cells previously exposed to IPTG along with unexposed cells were grown on galactose medium. It was observed that cells which are exposed to IPTG showed higher growth rate as compared to the ones which are not exposed to IPTG (Fig 1a). The percentage relative reduction in growth rate or burden for the cells which were not pre-exposed to IPTG in pre-culture was around 15% as compared to the ones which were exposed (Fig. 1b). The cells were subjected for β-galactosidase measurement on galactose environment. The β-galactosidase measurement was higher for cells which are pre-exposed to IPTG as compared to the cells which are not exposed (Fig. 1c). These results indicate that the cells with pre-induced lac operon enjoy phenotypic superiority on galactose environment as compared to the ones which are not induced. The biomass was also found to be higher for the pre-induced cells (Fig. 1d).


Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration.

Malakar P - Open Microbiol J (2015)

Phenotypic Growth Properties on Galactose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Solid Line with square represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Dashed Line with diamond represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Galactose (10g/L) was used in experiment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493661&req=5

Figure 1: Phenotypic Growth Properties on Galactose: (a) Growth rate for cells with and without pre-exposure to IPTG. (b) Percentage Relative reduction in growth rate for cells which are not exposed to IPTG in pre-culture as compared to the ones which are exposed to IPTG. (c) β-galactosidase activity for cells which are exposed to IPTG or not in pre-culture. (d) Growth Curve. Solid Line with square represents the cells which are exposed to 200 μM of IPTG in M9 glycerol medium in pre-culture. Dashed Line with diamond represents the cells which are not exposed to IPTG in M9 glycerol medium in pre-culture. Glycerol (1g/L) and Galactose (10g/L) was used in experiment.
Mentions: The focus of the study was to characterize the effect of pre-induced lac operon on glucose and galactose milieu along with the factors that influence the effect of pre-induced lac operon. Cells previously exposed to IPTG along with unexposed cells were grown on galactose medium. It was observed that cells which are exposed to IPTG showed higher growth rate as compared to the ones which are not exposed to IPTG (Fig 1a). The percentage relative reduction in growth rate or burden for the cells which were not pre-exposed to IPTG in pre-culture was around 15% as compared to the ones which were exposed (Fig. 1b). The cells were subjected for β-galactosidase measurement on galactose environment. The β-galactosidase measurement was higher for cells which are pre-exposed to IPTG as compared to the cells which are not exposed (Fig. 1c). These results indicate that the cells with pre-induced lac operon enjoy phenotypic superiority on galactose environment as compared to the ones which are not induced. The biomass was also found to be higher for the pre-induced cells (Fig. 1d).

Bottom Line: Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study.This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism.The above study will help in the better characterization of the pre culture effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India.

ABSTRACT
The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

No MeSH data available.


Related in: MedlinePlus