Limits...
Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis.

Li M, Liang P, Li Z, Wang Y, Zhang G, Gao H, Wen S, Tang L - Front Microbiol (2015)

Bottom Line: Disruption of intestinal microbial homeostasis impacted the integrity of mucosal epithelial layer, resulting in increased intestinal permeability.These outcomes were accompanied by overexpression of Muc2, significant decrease of SIgA secretion, and overproduction of defensins and inflammatory cytokines.The effects of BCT are comparable to that of FMT, especially in normalizing the intestinal levels of Muc2, SIgA, and defensins.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University Dalian, China ; Key Microecology Laboratory of Liaoning Province Dalian, China.

ABSTRACT
Fecal microbiota transplantation (FMT) is a promising therapy, despite some reports of adverse side effects. Bacterial consortia transplantation (BCT) for targeted restoration of the intestinal ecosystem is considered a relatively safe and simple procedure. However, no systematic research has assessed the effects of FMT and BCT on immune responses of intestinal mucosal barrier in patients. We conducted complementary studies in animal models on the effects of FMT and BCT, and provide recommendations for improving the clinical outcomes of these treatments. To establish the dysbiosis model, male BALB/c mice were treated with ceftriaxone intra-gastrically for 7 days. After that, FMT and BCT were performed on ceftriaxone-treated mice for 3 consecutive days to rebuild the intestinal ecosystem. Post-FMT and post-BCT changes of the intestinal microbial community and mucosal barrier functions were investigated and compared. Disruption of intestinal microbial homeostasis impacted the integrity of mucosal epithelial layer, resulting in increased intestinal permeability. These outcomes were accompanied by overexpression of Muc2, significant decrease of SIgA secretion, and overproduction of defensins and inflammatory cytokines. After FMT and BCT, the intestinal microbiota recovered quickly, this was associated with better reconstruction of mucosal barriers and re-establishment of immune networks compared with spontaneous recovery (SR). Although based on a short-term study, our results suggest that FMT and BCT promote the re-establishment of intestinal microbial communities in mice with antibiotic-induced dysbiosis, and contribute to the temporal and spatial interactions between microbiota and mucosal barriers. The effects of BCT are comparable to that of FMT, especially in normalizing the intestinal levels of Muc2, SIgA, and defensins.

No MeSH data available.


Related in: MedlinePlus

Post-FMT or BCT changes of intestinal SIgA, defensins and serum inflammatory cytokines. The concentration of SIgA (A), α-defensin 5 (B), 6 (C), β-defensin 1 (D), and 2 (E) in intestinal mucus of mice, and the serum levels of IL1-β (F), IL-6 (G), TNF-α (H) in mice post-FMT or BCT were measured by ELISA. Values are means ± SD of 5 mice per group. *, FMT compared with SR p < 0.05. Δ, BCT compared with FMT p < 0.05. The X axes indicate days after ceftriaxone treatment.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493656&req=5

Figure 6: Post-FMT or BCT changes of intestinal SIgA, defensins and serum inflammatory cytokines. The concentration of SIgA (A), α-defensin 5 (B), 6 (C), β-defensin 1 (D), and 2 (E) in intestinal mucus of mice, and the serum levels of IL1-β (F), IL-6 (G), TNF-α (H) in mice post-FMT or BCT were measured by ELISA. Values are means ± SD of 5 mice per group. *, FMT compared with SR p < 0.05. Δ, BCT compared with FMT p < 0.05. The X axes indicate days after ceftriaxone treatment.

Mentions: An increasing SIgA concentration was observed in the intestinal mucus of mice during recovery (Figure 6A). Among the different experimental groups, the BCT group showed a clearly superior recovery. On the 3rd and 7th day, the concentration of SIgA in the BCT group was significantly higher than in the SR group (p = 0.0230, p = 0.0340), and was higher than the FMT group by the 7th day (p = 0.0320). The concentration of SIgA in the FMT group recovered faster than the SR group, although the differences on the 3rd and 14th day were not significant statistically.


Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis.

Li M, Liang P, Li Z, Wang Y, Zhang G, Gao H, Wen S, Tang L - Front Microbiol (2015)

Post-FMT or BCT changes of intestinal SIgA, defensins and serum inflammatory cytokines. The concentration of SIgA (A), α-defensin 5 (B), 6 (C), β-defensin 1 (D), and 2 (E) in intestinal mucus of mice, and the serum levels of IL1-β (F), IL-6 (G), TNF-α (H) in mice post-FMT or BCT were measured by ELISA. Values are means ± SD of 5 mice per group. *, FMT compared with SR p < 0.05. Δ, BCT compared with FMT p < 0.05. The X axes indicate days after ceftriaxone treatment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493656&req=5

Figure 6: Post-FMT or BCT changes of intestinal SIgA, defensins and serum inflammatory cytokines. The concentration of SIgA (A), α-defensin 5 (B), 6 (C), β-defensin 1 (D), and 2 (E) in intestinal mucus of mice, and the serum levels of IL1-β (F), IL-6 (G), TNF-α (H) in mice post-FMT or BCT were measured by ELISA. Values are means ± SD of 5 mice per group. *, FMT compared with SR p < 0.05. Δ, BCT compared with FMT p < 0.05. The X axes indicate days after ceftriaxone treatment.
Mentions: An increasing SIgA concentration was observed in the intestinal mucus of mice during recovery (Figure 6A). Among the different experimental groups, the BCT group showed a clearly superior recovery. On the 3rd and 7th day, the concentration of SIgA in the BCT group was significantly higher than in the SR group (p = 0.0230, p = 0.0340), and was higher than the FMT group by the 7th day (p = 0.0320). The concentration of SIgA in the FMT group recovered faster than the SR group, although the differences on the 3rd and 14th day were not significant statistically.

Bottom Line: Disruption of intestinal microbial homeostasis impacted the integrity of mucosal epithelial layer, resulting in increased intestinal permeability.These outcomes were accompanied by overexpression of Muc2, significant decrease of SIgA secretion, and overproduction of defensins and inflammatory cytokines.The effects of BCT are comparable to that of FMT, especially in normalizing the intestinal levels of Muc2, SIgA, and defensins.

View Article: PubMed Central - PubMed

Affiliation: Department of Microecology, School of Basic Medical Science, Dalian Medical University Dalian, China ; Key Microecology Laboratory of Liaoning Province Dalian, China.

ABSTRACT
Fecal microbiota transplantation (FMT) is a promising therapy, despite some reports of adverse side effects. Bacterial consortia transplantation (BCT) for targeted restoration of the intestinal ecosystem is considered a relatively safe and simple procedure. However, no systematic research has assessed the effects of FMT and BCT on immune responses of intestinal mucosal barrier in patients. We conducted complementary studies in animal models on the effects of FMT and BCT, and provide recommendations for improving the clinical outcomes of these treatments. To establish the dysbiosis model, male BALB/c mice were treated with ceftriaxone intra-gastrically for 7 days. After that, FMT and BCT were performed on ceftriaxone-treated mice for 3 consecutive days to rebuild the intestinal ecosystem. Post-FMT and post-BCT changes of the intestinal microbial community and mucosal barrier functions were investigated and compared. Disruption of intestinal microbial homeostasis impacted the integrity of mucosal epithelial layer, resulting in increased intestinal permeability. These outcomes were accompanied by overexpression of Muc2, significant decrease of SIgA secretion, and overproduction of defensins and inflammatory cytokines. After FMT and BCT, the intestinal microbiota recovered quickly, this was associated with better reconstruction of mucosal barriers and re-establishment of immune networks compared with spontaneous recovery (SR). Although based on a short-term study, our results suggest that FMT and BCT promote the re-establishment of intestinal microbial communities in mice with antibiotic-induced dysbiosis, and contribute to the temporal and spatial interactions between microbiota and mucosal barriers. The effects of BCT are comparable to that of FMT, especially in normalizing the intestinal levels of Muc2, SIgA, and defensins.

No MeSH data available.


Related in: MedlinePlus