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Spread of Enterococcal Surface Protein in Antibiotic Resistant Entero-coccus faecium and Enterococcus faecalis isolates from Urinary Tract Infections.

Kafil HS, Mobarez AM - Open Microbiol J (2015)

Bottom Line: The esp gene was found in 76.1% of E. faecium isolates and 77.9% of E. faecalis isolate.There were significant correlation between esp positive E. faecium and resistance to Vancomycin (p<0.01), also in E.faecalis we found correlation between esp positive and resistance to Ampicillin, Chloramphenicol and Tetracycline (p<0.01, p<0.01, p<0.01 respectively).Also, our finding showed significant correlation between resistance to antibiotics and presence of esp in Enterococci.

View Article: PubMed Central - PubMed

Affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT
Enterococci rank among leading cause of nosocomial bacteremia and urinary tract infection in hospital and community acquired infections. Several traits that may contribute to enhanced virulence have been identified in Enterococci. Extracellular surface protein (Esp) is a virulence factor that contributes in biofilm formation and resistance to environmental stresses. In this study we aimed to determine occurrence of esp in E. faecium and E. faecalis isolates isolated from urinary tract infections and to investigate whether there is any correlation between presence of esp and antibiotic resistance. One hundred and sixty six isolates were collected from patients with UTI and after identification by biochemical and PCR, antibiotic resistances were examined. The presence of esp was investigated by primer-specific PCR. 43.3% of isolates identified as E. faecium and 56.7% as E. faecalis. The esp gene was found in 76.1% of E. faecium isolates and 77.9% of E. faecalis isolate. There were significant correlation between esp positive E. faecium and resistance to Vancomycin (p<0.01), also in E.faecalis we found correlation between esp positive and resistance to Ampicillin, Chloramphenicol and Tetracycline (p<0.01, p<0.01, p<0.01 respectively). Occurrence of esp in our isolates from urinary tract infection was high that indicates importance of this gene in urinary tract infections and shows importance of ability to forming biofilm and hydrophobicity of surface of Enterococci for causing urinary infection by Enterococci. Also, our finding showed significant correlation between resistance to antibiotics and presence of esp in Enterococci.

No MeSH data available.


Related in: MedlinePlus

E.faecalis Antibiotic resistance properties in Total and Esp positive strains. Sens: Sensitive; int: intermediate; res: resistance.
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Figure 2: E.faecalis Antibiotic resistance properties in Total and Esp positive strains. Sens: Sensitive; int: intermediate; res: resistance.

Mentions: From 166 clinical isolates of urinary tract infections of Enterococci, 59.7% of isolates were from females and 40.3% from males. By biochemical differentiation and PCR for specific genes, 43.3% of isolates were identified as E. faecium and 56.7% as E. faecalis. Antibiotic resistance of strain described by disc diffusion (Figs. 1 and 2) and the presence of esp gene was identified in 77.1% of all isolates of Enterococci. esp gene was identified in 76.1% of E. faecium and 77.9% of E. faecalis isolates (Fig. 3). There was not any significant difference between presence of esp gene in E. faecalis and E. faecium isolates (P> 0.05). Correlation of resistance to antibiotic and presence of the esp genes was investigated. In E. feacium, correlation was found between presence of the esp and resistance to Vancomycin (p<0.01) (Fig. 1). In E. faecalis we revealed correlation between resistance to Ampicillin, Chloramphenicol and Tetracycline (p<0.01, p<0.01 and p= 0.006 respectively) and presence of esp gene (Fig. 2).


Spread of Enterococcal Surface Protein in Antibiotic Resistant Entero-coccus faecium and Enterococcus faecalis isolates from Urinary Tract Infections.

Kafil HS, Mobarez AM - Open Microbiol J (2015)

E.faecalis Antibiotic resistance properties in Total and Esp positive strains. Sens: Sensitive; int: intermediate; res: resistance.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493631&req=5

Figure 2: E.faecalis Antibiotic resistance properties in Total and Esp positive strains. Sens: Sensitive; int: intermediate; res: resistance.
Mentions: From 166 clinical isolates of urinary tract infections of Enterococci, 59.7% of isolates were from females and 40.3% from males. By biochemical differentiation and PCR for specific genes, 43.3% of isolates were identified as E. faecium and 56.7% as E. faecalis. Antibiotic resistance of strain described by disc diffusion (Figs. 1 and 2) and the presence of esp gene was identified in 77.1% of all isolates of Enterococci. esp gene was identified in 76.1% of E. faecium and 77.9% of E. faecalis isolates (Fig. 3). There was not any significant difference between presence of esp gene in E. faecalis and E. faecium isolates (P> 0.05). Correlation of resistance to antibiotic and presence of the esp genes was investigated. In E. feacium, correlation was found between presence of the esp and resistance to Vancomycin (p<0.01) (Fig. 1). In E. faecalis we revealed correlation between resistance to Ampicillin, Chloramphenicol and Tetracycline (p<0.01, p<0.01 and p= 0.006 respectively) and presence of esp gene (Fig. 2).

Bottom Line: The esp gene was found in 76.1% of E. faecium isolates and 77.9% of E. faecalis isolate.There were significant correlation between esp positive E. faecium and resistance to Vancomycin (p<0.01), also in E.faecalis we found correlation between esp positive and resistance to Ampicillin, Chloramphenicol and Tetracycline (p<0.01, p<0.01, p<0.01 respectively).Also, our finding showed significant correlation between resistance to antibiotics and presence of esp in Enterococci.

View Article: PubMed Central - PubMed

Affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT
Enterococci rank among leading cause of nosocomial bacteremia and urinary tract infection in hospital and community acquired infections. Several traits that may contribute to enhanced virulence have been identified in Enterococci. Extracellular surface protein (Esp) is a virulence factor that contributes in biofilm formation and resistance to environmental stresses. In this study we aimed to determine occurrence of esp in E. faecium and E. faecalis isolates isolated from urinary tract infections and to investigate whether there is any correlation between presence of esp and antibiotic resistance. One hundred and sixty six isolates were collected from patients with UTI and after identification by biochemical and PCR, antibiotic resistances were examined. The presence of esp was investigated by primer-specific PCR. 43.3% of isolates identified as E. faecium and 56.7% as E. faecalis. The esp gene was found in 76.1% of E. faecium isolates and 77.9% of E. faecalis isolate. There were significant correlation between esp positive E. faecium and resistance to Vancomycin (p<0.01), also in E.faecalis we found correlation between esp positive and resistance to Ampicillin, Chloramphenicol and Tetracycline (p<0.01, p<0.01, p<0.01 respectively). Occurrence of esp in our isolates from urinary tract infection was high that indicates importance of this gene in urinary tract infections and shows importance of ability to forming biofilm and hydrophobicity of surface of Enterococci for causing urinary infection by Enterococci. Also, our finding showed significant correlation between resistance to antibiotics and presence of esp in Enterococci.

No MeSH data available.


Related in: MedlinePlus