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Rhein prevents endotoxin-induced acute kidney injury by inhibiting NF-κB activities.

Yu C, Qi D, Sun JF, Li P, Fan HY - Sci Rep (2015)

Bottom Line: For histopathological analysis, rhein effectively attenuated the severity of renal injury.Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis.All these results suggest that rhein has protective effects on endotoxin-induced kidney injury.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT
This study aimed to explore the effect and mechanisms of rhein on sepsis-induced acute kidney injury by injecting lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in vivo, and on LPS-induced HK-2 cells in vitro. For histopathological analysis, rhein effectively attenuated the severity of renal injury. Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis. Moreover, rhein could markedly attenuate circulating leukocyte infiltration and enhance phagocytic activity of macrophages partly impaired at 12 h after CLP. Rhein could enhance cell viability and suppresse the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells Furthermore, rhein down regulated the expression of phosphorylated NF-κB p65, IκBα and IKKβ stimulated by LPS both in vivo and in vitro. All these results suggest that rhein has protective effects on endotoxin-induced kidney injury. The underlying mechanism of rhein on anti-endotoxin kidney injury may be closely related with its anti-inflammatory and immunomodulatory properties by decreasing NF-κB activation through restraining the expression and phosphorylation of the relevant proteins in NF-κB signal pathway, hindering transcription of NF-κB p65.These evidence suggest that rhein has a potential application to treat endotoxemia-associated acute kidney injury.

No MeSH data available.


Related in: MedlinePlus

The effect of rhein on NF-κB signal pathway in LPS-induced HK-2 cells.The expression of NF-κB p65, p-IκBα and phospho-IKKβ were assessed by Western blot analysis. β-actin was used as an internal control. Results are expressed as fold increase over control group. Data are represented as mean ± SD of three independent experiments. ##p < 0.01 vs. control group, **p < 0.01 vs. LPS alone.
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f13: The effect of rhein on NF-κB signal pathway in LPS-induced HK-2 cells.The expression of NF-κB p65, p-IκBα and phospho-IKKβ were assessed by Western blot analysis. β-actin was used as an internal control. Results are expressed as fold increase over control group. Data are represented as mean ± SD of three independent experiments. ##p < 0.01 vs. control group, **p < 0.01 vs. LPS alone.

Mentions: To verify whether the actions of rhein attribute to the inhibition of NF-κB signal pathway, the key protein molecules were detected by western blot. As shown in Fig. 13, rhein significantly suppressed the expression of phosphorylated NF-κB p65, IKKβ and IκBα induced by LPS in a dose-dependent manner.


Rhein prevents endotoxin-induced acute kidney injury by inhibiting NF-κB activities.

Yu C, Qi D, Sun JF, Li P, Fan HY - Sci Rep (2015)

The effect of rhein on NF-κB signal pathway in LPS-induced HK-2 cells.The expression of NF-κB p65, p-IκBα and phospho-IKKβ were assessed by Western blot analysis. β-actin was used as an internal control. Results are expressed as fold increase over control group. Data are represented as mean ± SD of three independent experiments. ##p < 0.01 vs. control group, **p < 0.01 vs. LPS alone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493574&req=5

f13: The effect of rhein on NF-κB signal pathway in LPS-induced HK-2 cells.The expression of NF-κB p65, p-IκBα and phospho-IKKβ were assessed by Western blot analysis. β-actin was used as an internal control. Results are expressed as fold increase over control group. Data are represented as mean ± SD of three independent experiments. ##p < 0.01 vs. control group, **p < 0.01 vs. LPS alone.
Mentions: To verify whether the actions of rhein attribute to the inhibition of NF-κB signal pathway, the key protein molecules were detected by western blot. As shown in Fig. 13, rhein significantly suppressed the expression of phosphorylated NF-κB p65, IKKβ and IκBα induced by LPS in a dose-dependent manner.

Bottom Line: For histopathological analysis, rhein effectively attenuated the severity of renal injury.Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis.All these results suggest that rhein has protective effects on endotoxin-induced kidney injury.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT
This study aimed to explore the effect and mechanisms of rhein on sepsis-induced acute kidney injury by injecting lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in vivo, and on LPS-induced HK-2 cells in vitro. For histopathological analysis, rhein effectively attenuated the severity of renal injury. Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis. Moreover, rhein could markedly attenuate circulating leukocyte infiltration and enhance phagocytic activity of macrophages partly impaired at 12 h after CLP. Rhein could enhance cell viability and suppresse the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells Furthermore, rhein down regulated the expression of phosphorylated NF-κB p65, IκBα and IKKβ stimulated by LPS both in vivo and in vitro. All these results suggest that rhein has protective effects on endotoxin-induced kidney injury. The underlying mechanism of rhein on anti-endotoxin kidney injury may be closely related with its anti-inflammatory and immunomodulatory properties by decreasing NF-κB activation through restraining the expression and phosphorylation of the relevant proteins in NF-κB signal pathway, hindering transcription of NF-κB p65.These evidence suggest that rhein has a potential application to treat endotoxemia-associated acute kidney injury.

No MeSH data available.


Related in: MedlinePlus