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Rhein prevents endotoxin-induced acute kidney injury by inhibiting NF-κB activities.

Yu C, Qi D, Sun JF, Li P, Fan HY - Sci Rep (2015)

Bottom Line: For histopathological analysis, rhein effectively attenuated the severity of renal injury.Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis.All these results suggest that rhein has protective effects on endotoxin-induced kidney injury.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT
This study aimed to explore the effect and mechanisms of rhein on sepsis-induced acute kidney injury by injecting lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in vivo, and on LPS-induced HK-2 cells in vitro. For histopathological analysis, rhein effectively attenuated the severity of renal injury. Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis. Moreover, rhein could markedly attenuate circulating leukocyte infiltration and enhance phagocytic activity of macrophages partly impaired at 12 h after CLP. Rhein could enhance cell viability and suppresse the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells Furthermore, rhein down regulated the expression of phosphorylated NF-κB p65, IκBα and IKKβ stimulated by LPS both in vivo and in vitro. All these results suggest that rhein has protective effects on endotoxin-induced kidney injury. The underlying mechanism of rhein on anti-endotoxin kidney injury may be closely related with its anti-inflammatory and immunomodulatory properties by decreasing NF-κB activation through restraining the expression and phosphorylation of the relevant proteins in NF-κB signal pathway, hindering transcription of NF-κB p65.These evidence suggest that rhein has a potential application to treat endotoxemia-associated acute kidney injury.

No MeSH data available.


Related in: MedlinePlus

The effect of rhein on MCP-1 (A) and IL-8 (B) release induced by LPS in HK-2 cells. Cells were treated with LPS with or without rhein (10, 20, and 40 μM) for 24 h. 100 μl of culture medium in each group was taken out to measure the levels of MCP-1 and IL-8 using ELISA kits. Data are represented as mean ± SD of three independent experiments. ###p < 0.001 vs. control group, *p < 0.05, **p < 0.01 vs. LPS alone.
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f12: The effect of rhein on MCP-1 (A) and IL-8 (B) release induced by LPS in HK-2 cells. Cells were treated with LPS with or without rhein (10, 20, and 40 μM) for 24 h. 100 μl of culture medium in each group was taken out to measure the levels of MCP-1 and IL-8 using ELISA kits. Data are represented as mean ± SD of three independent experiments. ###p < 0.001 vs. control group, *p < 0.05, **p < 0.01 vs. LPS alone.

Mentions: The levels of MCP-1 and IL-8 were measured by ELISA after 24 h treated with LPS with or without rhein. As shown in Fig. 12, rhein suppressed the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells in a concentration-dependent manner.


Rhein prevents endotoxin-induced acute kidney injury by inhibiting NF-κB activities.

Yu C, Qi D, Sun JF, Li P, Fan HY - Sci Rep (2015)

The effect of rhein on MCP-1 (A) and IL-8 (B) release induced by LPS in HK-2 cells. Cells were treated with LPS with or without rhein (10, 20, and 40 μM) for 24 h. 100 μl of culture medium in each group was taken out to measure the levels of MCP-1 and IL-8 using ELISA kits. Data are represented as mean ± SD of three independent experiments. ###p < 0.001 vs. control group, *p < 0.05, **p < 0.01 vs. LPS alone.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493574&req=5

f12: The effect of rhein on MCP-1 (A) and IL-8 (B) release induced by LPS in HK-2 cells. Cells were treated with LPS with or without rhein (10, 20, and 40 μM) for 24 h. 100 μl of culture medium in each group was taken out to measure the levels of MCP-1 and IL-8 using ELISA kits. Data are represented as mean ± SD of three independent experiments. ###p < 0.001 vs. control group, *p < 0.05, **p < 0.01 vs. LPS alone.
Mentions: The levels of MCP-1 and IL-8 were measured by ELISA after 24 h treated with LPS with or without rhein. As shown in Fig. 12, rhein suppressed the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells in a concentration-dependent manner.

Bottom Line: For histopathological analysis, rhein effectively attenuated the severity of renal injury.Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis.All these results suggest that rhein has protective effects on endotoxin-induced kidney injury.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT
This study aimed to explore the effect and mechanisms of rhein on sepsis-induced acute kidney injury by injecting lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in vivo, and on LPS-induced HK-2 cells in vitro. For histopathological analysis, rhein effectively attenuated the severity of renal injury. Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis. Moreover, rhein could markedly attenuate circulating leukocyte infiltration and enhance phagocytic activity of macrophages partly impaired at 12 h after CLP. Rhein could enhance cell viability and suppresse the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells Furthermore, rhein down regulated the expression of phosphorylated NF-κB p65, IκBα and IKKβ stimulated by LPS both in vivo and in vitro. All these results suggest that rhein has protective effects on endotoxin-induced kidney injury. The underlying mechanism of rhein on anti-endotoxin kidney injury may be closely related with its anti-inflammatory and immunomodulatory properties by decreasing NF-κB activation through restraining the expression and phosphorylation of the relevant proteins in NF-κB signal pathway, hindering transcription of NF-κB p65.These evidence suggest that rhein has a potential application to treat endotoxemia-associated acute kidney injury.

No MeSH data available.


Related in: MedlinePlus