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Rhein prevents endotoxin-induced acute kidney injury by inhibiting NF-κB activities.

Yu C, Qi D, Sun JF, Li P, Fan HY - Sci Rep (2015)

Bottom Line: For histopathological analysis, rhein effectively attenuated the severity of renal injury.Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis.All these results suggest that rhein has protective effects on endotoxin-induced kidney injury.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT
This study aimed to explore the effect and mechanisms of rhein on sepsis-induced acute kidney injury by injecting lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in vivo, and on LPS-induced HK-2 cells in vitro. For histopathological analysis, rhein effectively attenuated the severity of renal injury. Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis. Moreover, rhein could markedly attenuate circulating leukocyte infiltration and enhance phagocytic activity of macrophages partly impaired at 12 h after CLP. Rhein could enhance cell viability and suppresse the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells Furthermore, rhein down regulated the expression of phosphorylated NF-κB p65, IκBα and IKKβ stimulated by LPS both in vivo and in vitro. All these results suggest that rhein has protective effects on endotoxin-induced kidney injury. The underlying mechanism of rhein on anti-endotoxin kidney injury may be closely related with its anti-inflammatory and immunomodulatory properties by decreasing NF-κB activation through restraining the expression and phosphorylation of the relevant proteins in NF-κB signal pathway, hindering transcription of NF-κB p65.These evidence suggest that rhein has a potential application to treat endotoxemia-associated acute kidney injury.

No MeSH data available.


Related in: MedlinePlus

The chemical structure of rhein.(C15H8O6, molecular weight = 284.22).
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f1: The chemical structure of rhein.(C15H8O6, molecular weight = 284.22).

Mentions: Rhubarb (Rheum officinale, Polygonaceae), which is one of popular traditional Chinese herbal medicine, has been widely used for the treatment of renal diseases and infectious inflammation in traditional Chinese medicine for many years15. Rhein (4, 5- dihydroxyanthraquinone-2-carboxylic acid, its chemical structure is shown in Fig. 1) is one of the most important bioactive anthraquinone derivatives from rhubarb. Recent studies have shown that rhein could inhibit NF-κB activation and sequentially suppresses its downstream inflammatory cytokines transcriptions in lipopolysaccharide (LPS)-activated macrophages16. Numerous earlier reports have identified that rhein can ameliorate glomerular hypertrophy, reduce urinary protein, suppress renal fibrosis in diabetic nephropathy, and improve renal function, reduce renal cell apoptosis to protect against further progression of chronic renal failure1718192021.


Rhein prevents endotoxin-induced acute kidney injury by inhibiting NF-κB activities.

Yu C, Qi D, Sun JF, Li P, Fan HY - Sci Rep (2015)

The chemical structure of rhein.(C15H8O6, molecular weight = 284.22).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493574&req=5

f1: The chemical structure of rhein.(C15H8O6, molecular weight = 284.22).
Mentions: Rhubarb (Rheum officinale, Polygonaceae), which is one of popular traditional Chinese herbal medicine, has been widely used for the treatment of renal diseases and infectious inflammation in traditional Chinese medicine for many years15. Rhein (4, 5- dihydroxyanthraquinone-2-carboxylic acid, its chemical structure is shown in Fig. 1) is one of the most important bioactive anthraquinone derivatives from rhubarb. Recent studies have shown that rhein could inhibit NF-κB activation and sequentially suppresses its downstream inflammatory cytokines transcriptions in lipopolysaccharide (LPS)-activated macrophages16. Numerous earlier reports have identified that rhein can ameliorate glomerular hypertrophy, reduce urinary protein, suppress renal fibrosis in diabetic nephropathy, and improve renal function, reduce renal cell apoptosis to protect against further progression of chronic renal failure1718192021.

Bottom Line: For histopathological analysis, rhein effectively attenuated the severity of renal injury.Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis.All these results suggest that rhein has protective effects on endotoxin-induced kidney injury.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT
This study aimed to explore the effect and mechanisms of rhein on sepsis-induced acute kidney injury by injecting lipopolysaccharide (LPS) and cecal ligation and puncture (CLP) in vivo, and on LPS-induced HK-2 cells in vitro. For histopathological analysis, rhein effectively attenuated the severity of renal injury. Rhein could significantly decrease concentration of BUN and SCr and level of TNF-α and IL-1β in two different mouse models of experimental sepsis. Moreover, rhein could markedly attenuate circulating leukocyte infiltration and enhance phagocytic activity of macrophages partly impaired at 12 h after CLP. Rhein could enhance cell viability and suppresse the release of MCP-1 and IL-8 in LPS-stimulated HK-2 cells Furthermore, rhein down regulated the expression of phosphorylated NF-κB p65, IκBα and IKKβ stimulated by LPS both in vivo and in vitro. All these results suggest that rhein has protective effects on endotoxin-induced kidney injury. The underlying mechanism of rhein on anti-endotoxin kidney injury may be closely related with its anti-inflammatory and immunomodulatory properties by decreasing NF-κB activation through restraining the expression and phosphorylation of the relevant proteins in NF-κB signal pathway, hindering transcription of NF-κB p65.These evidence suggest that rhein has a potential application to treat endotoxemia-associated acute kidney injury.

No MeSH data available.


Related in: MedlinePlus