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Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus

Representative chromatograms of head-space volatile compounds from cotton plants that were infested by cotton bollworm for 48 h(A), untreated cotton plants (B), or an empty container (a pot of soil without plants) (C). Detail information of volatile compounds is shown in Table 1. IS, internal standard (ethyl caprate).
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f9: Representative chromatograms of head-space volatile compounds from cotton plants that were infested by cotton bollworm for 48 h(A), untreated cotton plants (B), or an empty container (a pot of soil without plants) (C). Detail information of volatile compounds is shown in Table 1. IS, internal standard (ethyl caprate).

Mentions: Based on analysis of the expression patterns of genes encoding enzymes responsible for the biosynthesis of volatiles, we further compared volatile profiles between treatment and control. Representative chromatograms of head-space volatile compounds from cotton bollworm-damaged cotton plants are shown in Fig. 9. Lipoxygenase-derived green leaf volatiles, including (Z)-3-hexenol, (Z)-3-hexenyl acetate and (E)-2-hexenyl acetate, and a number of terpenoids such as limonene, caryophyllene, and DMNT were emitted transiently in relatively large amounts during early stages of damage. As damage progressed, however, there was increased production of several other terpenes including (E)-β-ocimene, linalool, δ-cadinene and TMTT, which had been released only in relatively small amounts during early stages of damage (Fig. 9; Table 1). Cotton VOCs changes in CBW-infested plants were basically consistent with the results of transcriptome analysis, indicating cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile in response to CBW larvae feeding.


Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Representative chromatograms of head-space volatile compounds from cotton plants that were infested by cotton bollworm for 48 h(A), untreated cotton plants (B), or an empty container (a pot of soil without plants) (C). Detail information of volatile compounds is shown in Table 1. IS, internal standard (ethyl caprate).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493570&req=5

f9: Representative chromatograms of head-space volatile compounds from cotton plants that were infested by cotton bollworm for 48 h(A), untreated cotton plants (B), or an empty container (a pot of soil without plants) (C). Detail information of volatile compounds is shown in Table 1. IS, internal standard (ethyl caprate).
Mentions: Based on analysis of the expression patterns of genes encoding enzymes responsible for the biosynthesis of volatiles, we further compared volatile profiles between treatment and control. Representative chromatograms of head-space volatile compounds from cotton bollworm-damaged cotton plants are shown in Fig. 9. Lipoxygenase-derived green leaf volatiles, including (Z)-3-hexenol, (Z)-3-hexenyl acetate and (E)-2-hexenyl acetate, and a number of terpenoids such as limonene, caryophyllene, and DMNT were emitted transiently in relatively large amounts during early stages of damage. As damage progressed, however, there was increased production of several other terpenes including (E)-β-ocimene, linalool, δ-cadinene and TMTT, which had been released only in relatively small amounts during early stages of damage (Fig. 9; Table 1). Cotton VOCs changes in CBW-infested plants were basically consistent with the results of transcriptome analysis, indicating cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile in response to CBW larvae feeding.

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus