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Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus

Examples of KEGG pathways found for transcripts associated with phytohormone signaling.Each box shows enzymes involved in each section of the pathway. Genes highlighted in red were up-regulated, and those in green were down-regulated.
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f6: Examples of KEGG pathways found for transcripts associated with phytohormone signaling.Each box shows enzymes involved in each section of the pathway. Genes highlighted in red were up-regulated, and those in green were down-regulated.

Mentions: Based on the central role of phytohormones in plant defense responses to insect herbivore attack, we investigated the profiles of transcripts associated with signaling molecules and phytohormones: auxin, cytokinin, abscisic acid (ABA), brassinosteroids (BR), gibberellic acid (GA), ET, JA, and SA (Fig. 6, Supplementary Figure S2 and Supplementary Table S4 and S5).


Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Examples of KEGG pathways found for transcripts associated with phytohormone signaling.Each box shows enzymes involved in each section of the pathway. Genes highlighted in red were up-regulated, and those in green were down-regulated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493570&req=5

f6: Examples of KEGG pathways found for transcripts associated with phytohormone signaling.Each box shows enzymes involved in each section of the pathway. Genes highlighted in red were up-regulated, and those in green were down-regulated.
Mentions: Based on the central role of phytohormones in plant defense responses to insect herbivore attack, we investigated the profiles of transcripts associated with signaling molecules and phytohormones: auxin, cytokinin, abscisic acid (ABA), brassinosteroids (BR), gibberellic acid (GA), ET, JA, and SA (Fig. 6, Supplementary Figure S2 and Supplementary Table S4 and S5).

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus