Limits...
Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus

Clusters of DEGs defined by the K-means algorithm and grouped based on the dynamics of expression changes during the time course.Red arrows: up-regulated expression at one time point relative to the control or the previous time point; green arrows: down-regulated expression at one time point. Arrows are followed by cluster designation and the number of genes in each cluster. P1, P2 and P3 are the top three clusters.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493570&req=5

f5: Clusters of DEGs defined by the K-means algorithm and grouped based on the dynamics of expression changes during the time course.Red arrows: up-regulated expression at one time point relative to the control or the previous time point; green arrows: down-regulated expression at one time point. Arrows are followed by cluster designation and the number of genes in each cluster. P1, P2 and P3 are the top three clusters.

Mentions: Among 7,811 transcripts associated with a q-value of less than or equal to 0.05 for at least one time point, 1,971 were placed into 16 expression clusters based on the temporal patterns in their expression profiles as identified by K-means clustering (Fig. 5). Although 597 genes displayed a rapid transient up-regulation within 6 h upon onset of herbivory (Clusters E - H), a majority of 110 genes peaked at 12 h (clusters C and cluster D). Another group of DEGs peaked at 24 h (cluster B), whereas five genes were up-regulated early during the treatment and maintained high expression levels relative to control plants (cluster A). Similarly, many down-regulated genes displayed transient expression profiles (Clusters I - O), although 119 down-regulated genes maintained lower expression levels over the entire time course analyzed (cluster P). In order to produce a global description of gene functions enriched in the top three clusters of similarly regulated transcripts, we generated an overview of GO annotation (Supplementary Table S3).


Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Clusters of DEGs defined by the K-means algorithm and grouped based on the dynamics of expression changes during the time course.Red arrows: up-regulated expression at one time point relative to the control or the previous time point; green arrows: down-regulated expression at one time point. Arrows are followed by cluster designation and the number of genes in each cluster. P1, P2 and P3 are the top three clusters.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493570&req=5

f5: Clusters of DEGs defined by the K-means algorithm and grouped based on the dynamics of expression changes during the time course.Red arrows: up-regulated expression at one time point relative to the control or the previous time point; green arrows: down-regulated expression at one time point. Arrows are followed by cluster designation and the number of genes in each cluster. P1, P2 and P3 are the top three clusters.
Mentions: Among 7,811 transcripts associated with a q-value of less than or equal to 0.05 for at least one time point, 1,971 were placed into 16 expression clusters based on the temporal patterns in their expression profiles as identified by K-means clustering (Fig. 5). Although 597 genes displayed a rapid transient up-regulation within 6 h upon onset of herbivory (Clusters E - H), a majority of 110 genes peaked at 12 h (clusters C and cluster D). Another group of DEGs peaked at 24 h (cluster B), whereas five genes were up-regulated early during the treatment and maintained high expression levels relative to control plants (cluster A). Similarly, many down-regulated genes displayed transient expression profiles (Clusters I - O), although 119 down-regulated genes maintained lower expression levels over the entire time course analyzed (cluster P). In order to produce a global description of gene functions enriched in the top three clusters of similarly regulated transcripts, we generated an overview of GO annotation (Supplementary Table S3).

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus