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Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus

Comparative analysis of changes in the cotton leaf transcriptome in response to cotton bollworm at different time-points.Genes associated with a q-value of less than or equal to 0.05 for at least one time point were used to construct the Venn diagram.
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f1: Comparative analysis of changes in the cotton leaf transcriptome in response to cotton bollworm at different time-points.Genes associated with a q-value of less than or equal to 0.05 for at least one time point were used to construct the Venn diagram.

Mentions: The number of genes associated with a q-value of less than or equal to 0.05 at each time point was found to range from 2,339 (1184 up- and 1155 down-regulated) after 12 h of CBW feeding to 5,042 (2,421 up- and 2,621 down-regulated array elements) after 48 h of herbivory (Supplementary Table S1 and S2). A Venn diagram was constructed to identify commonly and exclusively regulated genes in response to CBW infestation over the 48 h time course (Fig. 1). We observed that 9% genes were commonly expressed in response to CBW feeding over the 48 h time course. In addition, many genes were exclusively expressed at 6 h after the onset of CBW infestation, which indicates that these genes are likely to play roles in the herbivory-induced early signaling events. To evaluate the reproducibility of the microarray data, 15 genes were selected for qPCR analysis (Fig. 2). These included genes related to phytohormone biosynthesis and secondary metabolism, including one phenylpropanoid-biosynthesis gene, one ET-biosynthesis gene, two JA-biosynthesis genes, two flavonoid-biosynthesis genes and nine terpenoid-biosynthesis genes. For most of these genes, expression patterns tested by qPCR were highly consistent with the results of microarray analysis, indicating that the microarray assay can meet the further research requirements to identify target genes and key pathways involved in the activation of herbivory-induced indirect defense.


Dynamic transcriptome analysis and volatile profiling of Gossypium hirsutum in response to the cotton bollworm Helicoverpa armigera.

Huang XZ, Chen JY, Xiao HJ, Xiao YT, Wu J, Wu JX, Zhou JJ, Zhang YJ, Guo YY - Sci Rep (2015)

Comparative analysis of changes in the cotton leaf transcriptome in response to cotton bollworm at different time-points.Genes associated with a q-value of less than or equal to 0.05 for at least one time point were used to construct the Venn diagram.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493570&req=5

f1: Comparative analysis of changes in the cotton leaf transcriptome in response to cotton bollworm at different time-points.Genes associated with a q-value of less than or equal to 0.05 for at least one time point were used to construct the Venn diagram.
Mentions: The number of genes associated with a q-value of less than or equal to 0.05 at each time point was found to range from 2,339 (1184 up- and 1155 down-regulated) after 12 h of CBW feeding to 5,042 (2,421 up- and 2,621 down-regulated array elements) after 48 h of herbivory (Supplementary Table S1 and S2). A Venn diagram was constructed to identify commonly and exclusively regulated genes in response to CBW infestation over the 48 h time course (Fig. 1). We observed that 9% genes were commonly expressed in response to CBW feeding over the 48 h time course. In addition, many genes were exclusively expressed at 6 h after the onset of CBW infestation, which indicates that these genes are likely to play roles in the herbivory-induced early signaling events. To evaluate the reproducibility of the microarray data, 15 genes were selected for qPCR analysis (Fig. 2). These included genes related to phytohormone biosynthesis and secondary metabolism, including one phenylpropanoid-biosynthesis gene, one ET-biosynthesis gene, two JA-biosynthesis genes, two flavonoid-biosynthesis genes and nine terpenoid-biosynthesis genes. For most of these genes, expression patterns tested by qPCR were highly consistent with the results of microarray analysis, indicating that the microarray assay can meet the further research requirements to identify target genes and key pathways involved in the activation of herbivory-induced indirect defense.

Bottom Line: Cluster analysis identified several distinct temporal patterns of transcriptome changes.Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses.The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Plant Protection, Northwest A &F University, Yangling, Shaanxi 712100, China [2] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

ABSTRACT
In response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. However, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. Here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (CBW; Helicoverpa armigera) larvae infestation. Analysis of samples revealed that 1,969 transcripts were differentially expressed (log2/Ratio/ ≥ 2; q ≤ 0.05) after CBW infestation. Cluster analysis identified several distinct temporal patterns of transcriptome changes. Among CBW-induced genes, those associated with indirect defense and jasmonic acid pathway were clearly over-represented, indicating that these genes play important roles in CBW-induced defenses. The gas chromatography-mass spectrometry (GC-MS) analyses revealed that CBW infestation could induce cotton plants to release volatile compounds comprised lipoxygenase-derived green leaf volatiles and a number of terpenoid volatiles. Responding to CBW larvae infestation, cotton plants undergo drastic reprogramming of the transcriptome and the volatile profile. The present results increase our knowledge about insect herbivory-induced metabolic and biochemical processes in plants, which may help improve future studies on genes governing processes.

No MeSH data available.


Related in: MedlinePlus