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Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

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Related in: MedlinePlus

(a) Structure of the dansyl-unit (D) in oligonucleotide; (b) Sequences of ONTs. Internally dansyl-labeled ONTs (I-Ds) has a complementary sequence with II-X and III. X in II-X indicates dA, dG, dC, or T.
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biosensors-05-00337-f016: (a) Structure of the dansyl-unit (D) in oligonucleotide; (b) Sequences of ONTs. Internally dansyl-labeled ONTs (I-Ds) has a complementary sequence with II-X and III. X in II-X indicates dA, dG, dC, or T.

Mentions: Suzuki et al. have synthesized a nonnucleoside amidite block of dansyl fluorophore to prepare dansyl-modified oligonucleotides (ONTs) (Figure 16) [88]. The fluorescence intensities of dansyl-ONTs were specifically increased by the presence of adjacent guanosine residues but significantly reduced in a dansyl-flipping duplex. These changes were caused by a solvatochromism effect due to the number of guanines, which are hydrophobic functional groups, and the external environment of the dansyl group. The fluorescence intensities could be plotted as a function of the ONT concentrations and the increase in the fluorescence was observed to equimolar concentrations of target DNA. This duplex exhibited a higher melting temperature relative to the corresponding duplexes containing other base pairs. Similar changes in fluorescence could be detected upon hybridization of the ONTs with complementary RNAs.


Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

(a) Structure of the dansyl-unit (D) in oligonucleotide; (b) Sequences of ONTs. Internally dansyl-labeled ONTs (I-Ds) has a complementary sequence with II-X and III. X in II-X indicates dA, dG, dC, or T.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f016: (a) Structure of the dansyl-unit (D) in oligonucleotide; (b) Sequences of ONTs. Internally dansyl-labeled ONTs (I-Ds) has a complementary sequence with II-X and III. X in II-X indicates dA, dG, dC, or T.
Mentions: Suzuki et al. have synthesized a nonnucleoside amidite block of dansyl fluorophore to prepare dansyl-modified oligonucleotides (ONTs) (Figure 16) [88]. The fluorescence intensities of dansyl-ONTs were specifically increased by the presence of adjacent guanosine residues but significantly reduced in a dansyl-flipping duplex. These changes were caused by a solvatochromism effect due to the number of guanines, which are hydrophobic functional groups, and the external environment of the dansyl group. The fluorescence intensities could be plotted as a function of the ONT concentrations and the increase in the fluorescence was observed to equimolar concentrations of target DNA. This duplex exhibited a higher melting temperature relative to the corresponding duplexes containing other base pairs. Similar changes in fluorescence could be detected upon hybridization of the ONTs with complementary RNAs.

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH
Related in: MedlinePlus