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Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

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Chemical structures of fluorescent molecular probes for the labeling of proteins.
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biosensors-05-00337-f010: Chemical structures of fluorescent molecular probes for the labeling of proteins.

Mentions: Biochemists need various ways to detect proteins with a high sensitivity and good binding linearity to facilitate both qualitative and quantitative analysis. Several fluorescent reagents have been developed for the detection of proteins in solution, such as fluorescamine and cyanine dyes (Figure 10) [74]. Fluorescamine does not produce fluorescence emission, but a strong green fluorescence at 495 nm is observed when excited at 395 nm after reaction with the primary amine in the protein.


Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Chemical structures of fluorescent molecular probes for the labeling of proteins.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f010: Chemical structures of fluorescent molecular probes for the labeling of proteins.
Mentions: Biochemists need various ways to detect proteins with a high sensitivity and good binding linearity to facilitate both qualitative and quantitative analysis. Several fluorescent reagents have been developed for the detection of proteins in solution, such as fluorescamine and cyanine dyes (Figure 10) [74]. Fluorescamine does not produce fluorescence emission, but a strong green fluorescence at 495 nm is observed when excited at 395 nm after reaction with the primary amine in the protein.

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH
Related in: MedlinePlus