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Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

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Related in: MedlinePlus

Chemical structures of zinc fluorescent probes.
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biosensors-05-00337-f009: Chemical structures of zinc fluorescent probes.

Mentions: The initial Zn2+ fluorescent indicators, such as Zinquin, were based on an 8-p-toluenesulfonamidoquinoline derivative shown in Figure 9 [59,60]. Zinquin ester readily traverses cell membranes and is thought to be hydrolyzed by cellular esterases in the cytoplasm. The resulting acid is deprotonated at intracellular pH, and the charged molecule has a decreased ability to cross the cell membrane and escape from the cell. Zinquin ester has been shown to be effective in the detection of zinc(II) in a variety of mammalian cells [61,62,63].


Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Chemical structures of zinc fluorescent probes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f009: Chemical structures of zinc fluorescent probes.
Mentions: The initial Zn2+ fluorescent indicators, such as Zinquin, were based on an 8-p-toluenesulfonamidoquinoline derivative shown in Figure 9 [59,60]. Zinquin ester readily traverses cell membranes and is thought to be hydrolyzed by cellular esterases in the cytoplasm. The resulting acid is deprotonated at intracellular pH, and the charged molecule has a decreased ability to cross the cell membrane and escape from the cell. Zinquin ester has been shown to be effective in the detection of zinc(II) in a variety of mammalian cells [61,62,63].

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH
Related in: MedlinePlus