Limits...
Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH

Related in: MedlinePlus

Chemical structures of magnesium fluorescent probes possessing an APTRA group.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f006: Chemical structures of magnesium fluorescent probes possessing an APTRA group.

Mentions: Mag-fura-2, shown in Figure 6, is the first fluorescent magnesium indictor, developed to visualize and understand intracellular Mg2+ distributions [35]. The binding site for Mg2+ in mag-fura-2 is the APTRA (O-aminophenol-N, N, O-triacetic acid) group, and its dissociation constant for Mg2+ is 1.9 mM. Mag-fura-2 is excited by UV light (λmax: 369 nm), and shows an increase in fluorescence intensity in the visible region upon Mg2+ binding. Mag-Fura-2 has been used to study the intracellular Mg2+ concentration in cells from the liver, heart, muscle, and nervous system [36,37,38,39].


Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Chemical structures of magnesium fluorescent probes possessing an APTRA group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f006: Chemical structures of magnesium fluorescent probes possessing an APTRA group.
Mentions: Mag-fura-2, shown in Figure 6, is the first fluorescent magnesium indictor, developed to visualize and understand intracellular Mg2+ distributions [35]. The binding site for Mg2+ in mag-fura-2 is the APTRA (O-aminophenol-N, N, O-triacetic acid) group, and its dissociation constant for Mg2+ is 1.9 mM. Mag-fura-2 is excited by UV light (λmax: 369 nm), and shows an increase in fluorescence intensity in the visible region upon Mg2+ binding. Mag-Fura-2 has been used to study the intracellular Mg2+ concentration in cells from the liver, heart, muscle, and nervous system [36,37,38,39].

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH
Related in: MedlinePlus