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Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

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Chemical structure of KFCA.
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biosensors-05-00337-f005: Chemical structure of KFCA.

Mentions: Near-infrared (NIR) fluorescent probes, which show emission peaks from 650 nm to 900 nm, have been developed because of the minimal NIR autofluorescence from biomolecules, as well as the deep tissue penetration of NIR light. NIR calcium probes based on cyanine or squaraine chromophores have been designed and synthesized [29,30]. However, these probes have either a low quantum yield or a poor ON/OFF fluorescence signal contrast. To overcome these limitations, Suzuki et al. reported the development of a BODIPY-based NIR fluorescent Ca2+ probe (KFCA), shown in Figure 5 [31]. This probe emits sharp fluorescence spectra with high extinction coefficients, high quantum yields (emission maximum; 670 nm, Φ: 0.24), and an excellent ON/OFF ratio (120-fold), and was successfully used in real-time dual-colour intracellular Ca2+ imaging.


Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Chemical structure of KFCA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f005: Chemical structure of KFCA.
Mentions: Near-infrared (NIR) fluorescent probes, which show emission peaks from 650 nm to 900 nm, have been developed because of the minimal NIR autofluorescence from biomolecules, as well as the deep tissue penetration of NIR light. NIR calcium probes based on cyanine or squaraine chromophores have been designed and synthesized [29,30]. However, these probes have either a low quantum yield or a poor ON/OFF fluorescence signal contrast. To overcome these limitations, Suzuki et al. reported the development of a BODIPY-based NIR fluorescent Ca2+ probe (KFCA), shown in Figure 5 [31]. This probe emits sharp fluorescence spectra with high extinction coefficients, high quantum yields (emission maximum; 670 nm, Φ: 0.24), and an excellent ON/OFF ratio (120-fold), and was successfully used in real-time dual-colour intracellular Ca2+ imaging.

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH
Related in: MedlinePlus