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Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

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Concept of fluorescent molecular probes and their biological applications.
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biosensors-05-00337-f001: Concept of fluorescent molecular probes and their biological applications.

Mentions: Several analytical methods are available for obtaining information about transmission in living cells, including absorption spectrometry, fluorescence spectrometry, electrochemical methods, chemical luminescence, and isotope-based methods. Particularly, fluorescence spectrometry is a highly sensitive and straightforward analytical method. Advances in optical instrumentation led to the development of specialized fluorescent imaging techniques such as fluorescence microscopy, confocal laser scanning microscopy, two photon excitation instruments, and evanescent excitation instruments. Recently, analytical scientists, organic chemists, and medical scientists have designed various types of fluorescent probes that emit a spectral response upon binding ions or neutral organic or inorganic molecules. These probes have enabled researchers to investigate the changes in intracellular free ions or the concentrations of molecules using fluorescent microscopy, flow cytometry, and fluorescent spectroscopy (Figure 1). Such fluorescence imaging techniques have benefited not only from the advancement of optical instruments, but also from the development of highly functional fluorescent probes based on new molecular designs.


Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.

Suzuki Y, Yokoyama K - Biosensors (Basel) (2015)

Concept of fluorescent molecular probes and their biological applications.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493553&req=5

biosensors-05-00337-f001: Concept of fluorescent molecular probes and their biological applications.
Mentions: Several analytical methods are available for obtaining information about transmission in living cells, including absorption spectrometry, fluorescence spectrometry, electrochemical methods, chemical luminescence, and isotope-based methods. Particularly, fluorescence spectrometry is a highly sensitive and straightforward analytical method. Advances in optical instrumentation led to the development of specialized fluorescent imaging techniques such as fluorescence microscopy, confocal laser scanning microscopy, two photon excitation instruments, and evanescent excitation instruments. Recently, analytical scientists, organic chemists, and medical scientists have designed various types of fluorescent probes that emit a spectral response upon binding ions or neutral organic or inorganic molecules. These probes have enabled researchers to investigate the changes in intracellular free ions or the concentrations of molecules using fluorescent microscopy, flow cytometry, and fluorescent spectroscopy (Figure 1). Such fluorescence imaging techniques have benefited not only from the advancement of optical instruments, but also from the development of highly functional fluorescent probes based on new molecular designs.

Bottom Line: This review is confined to sensors that use fluorescence to transmit biochemical information.Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors.To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan. suzuki-yoshio@aist.go.jp.

ABSTRACT
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.

Show MeSH
Related in: MedlinePlus