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Multi-Pixel Photon Counters for Optofluidic Characterization of Particles and Microalgae.

Asrar P, Sucur M, Hashemi N - Biosensors (Basel) (2015)

Bottom Line: The forward scattering emissions generated by samples passing through the interrogation region were carried through a multimode fiber, located in 135 degree with respect to the excitation fiber, and detected by a MPPC.Larger particles or cells demonstrated larger peak height and width, and consequently larger peak area.Different types of algae were also successfully characterized.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA. pouya@gatech.edu.

ABSTRACT
We have developed an optofluidic biosensor to study microscale particles and different species of microalgae. The system is comprised of a microchannel with a set of chevron-shaped grooves. The chevrons allows for hydrodynamic focusing of the core stream in the center using a sheath fluid. The device is equipped with a new generation of highly sensitive photodetectors, multi-pixel photon counter (MPPC), with high gain values and an extremely small footprint. Two different sizes of high intensity fluorescent microspheres and three different species of algae (Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana) were studied. The forward scattering emissions generated by samples passing through the interrogation region were carried through a multimode fiber, located in 135 degree with respect to the excitation fiber, and detected by a MPPC. The signal outputs obtained from each sample were collected using a data acquisition system and utilized for further statistical analysis. Larger particles or cells demonstrated larger peak height and width, and consequently larger peak area. The average signal output (integral of the peak) for Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana falls between the values found for the 3.2 and 10.2 μm beads. Different types of algae were also successfully characterized.

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Related in: MedlinePlus

The statistical analysis for three types of algae. The blue bar shows the average of SPDPs for Chlamydomonas reinhardtii strain 21 gr, red bar represents the average value for Chlamydomonas suppressor, and green bar shows the average value for Chlorella sorokiniana. The average of SPDPs is 0.58 V for Chlamydomonas reinhardtii strain 21 gr, 0.59 V Chlamydomonas suppressor, and 0.63 V for Chlorella sorokiniana. The standard deviation is shown on each column.
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biosensors-05-00308-f005: The statistical analysis for three types of algae. The blue bar shows the average of SPDPs for Chlamydomonas reinhardtii strain 21 gr, red bar represents the average value for Chlamydomonas suppressor, and green bar shows the average value for Chlorella sorokiniana. The average of SPDPs is 0.58 V for Chlamydomonas reinhardtii strain 21 gr, 0.59 V Chlamydomonas suppressor, and 0.63 V for Chlorella sorokiniana. The standard deviation is shown on each column.

Mentions: The signal output collected for Chlamydomonas reinhardtii strain 21 gr is very similar to Chlamydomonas suppressor while the signal output of Chlorella sorokiniana clearly has a higher magnitude compared to other two types of algae. This confirmed that Chlorella sorokiniana are larger compared to the other two types. Microscopy images confirmed these results with respect to the sizes of the algae. We were able to successfully detect the slight difference in size for Chlamydomonas reinhardtii strain 21 gr and Chlamydomonas suppressor using further statistical analysis as shown in Figure 5.


Multi-Pixel Photon Counters for Optofluidic Characterization of Particles and Microalgae.

Asrar P, Sucur M, Hashemi N - Biosensors (Basel) (2015)

The statistical analysis for three types of algae. The blue bar shows the average of SPDPs for Chlamydomonas reinhardtii strain 21 gr, red bar represents the average value for Chlamydomonas suppressor, and green bar shows the average value for Chlorella sorokiniana. The average of SPDPs is 0.58 V for Chlamydomonas reinhardtii strain 21 gr, 0.59 V Chlamydomonas suppressor, and 0.63 V for Chlorella sorokiniana. The standard deviation is shown on each column.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493551&req=5

biosensors-05-00308-f005: The statistical analysis for three types of algae. The blue bar shows the average of SPDPs for Chlamydomonas reinhardtii strain 21 gr, red bar represents the average value for Chlamydomonas suppressor, and green bar shows the average value for Chlorella sorokiniana. The average of SPDPs is 0.58 V for Chlamydomonas reinhardtii strain 21 gr, 0.59 V Chlamydomonas suppressor, and 0.63 V for Chlorella sorokiniana. The standard deviation is shown on each column.
Mentions: The signal output collected for Chlamydomonas reinhardtii strain 21 gr is very similar to Chlamydomonas suppressor while the signal output of Chlorella sorokiniana clearly has a higher magnitude compared to other two types of algae. This confirmed that Chlorella sorokiniana are larger compared to the other two types. Microscopy images confirmed these results with respect to the sizes of the algae. We were able to successfully detect the slight difference in size for Chlamydomonas reinhardtii strain 21 gr and Chlamydomonas suppressor using further statistical analysis as shown in Figure 5.

Bottom Line: The forward scattering emissions generated by samples passing through the interrogation region were carried through a multimode fiber, located in 135 degree with respect to the excitation fiber, and detected by a MPPC.Larger particles or cells demonstrated larger peak height and width, and consequently larger peak area.Different types of algae were also successfully characterized.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA. pouya@gatech.edu.

ABSTRACT
We have developed an optofluidic biosensor to study microscale particles and different species of microalgae. The system is comprised of a microchannel with a set of chevron-shaped grooves. The chevrons allows for hydrodynamic focusing of the core stream in the center using a sheath fluid. The device is equipped with a new generation of highly sensitive photodetectors, multi-pixel photon counter (MPPC), with high gain values and an extremely small footprint. Two different sizes of high intensity fluorescent microspheres and three different species of algae (Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana) were studied. The forward scattering emissions generated by samples passing through the interrogation region were carried through a multimode fiber, located in 135 degree with respect to the excitation fiber, and detected by a MPPC. The signal outputs obtained from each sample were collected using a data acquisition system and utilized for further statistical analysis. Larger particles or cells demonstrated larger peak height and width, and consequently larger peak area. The average signal output (integral of the peak) for Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana falls between the values found for the 3.2 and 10.2 μm beads. Different types of algae were also successfully characterized.

Show MeSH
Related in: MedlinePlus