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Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion.

Zagorodko O, Bouckaert J, Dumych T, Bilyy R, Larroulet I, Yanguas Serrano A, Alvarez Dorta D, Gouin SG, Dima SO, Oancea F, Boukherroub R, Szunerits S - Biosensors (Basel) (2015)

Bottom Line: In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner.We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa).This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

View Article: PubMed Central - PubMed

Affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. morjakzzz@gmail.com.

ABSTRACT
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

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(a) SPR sensogram of affinity interaction of E. coli UTI (108 cfu/mL) with Au and Au-HM interfaces; flow rate = 20 µL/min; (b) bar graph diagrams of the change in SPR signals of Au and Au-HM interfaces upon interaction for 30 min at a flow rate = 20 µL/min with UTI 89 and UTI89 Q133K.
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biosensors-05-00276-f002: (a) SPR sensogram of affinity interaction of E. coli UTI (108 cfu/mL) with Au and Au-HM interfaces; flow rate = 20 µL/min; (b) bar graph diagrams of the change in SPR signals of Au and Au-HM interfaces upon interaction for 30 min at a flow rate = 20 µL/min with UTI 89 and UTI89 Q133K.

Mentions: The interaction between E. coli (108 cfu/mL) and Au-HM interfaces was investigated in real time using label-free interaction analysis in an SPR assay format (Figure 2). Figure 2a shows the change in the SPR signal over 30 min on Au and Au-HM interfaces upon interaction with E. coli UTI89. The binding ability of E. coli UTI89 to Au-HM was significantly larger than on gold alone, in line with the well-known glycans-mediated increase in pathogen adhesion. To underline the specific aminoheptyl α-D-mannopyranoside-E. coli UTI89 interaction, the adhesion behavior of a strain called UTI89 Q133K, made dysfunctional for mannose binding through a glutamine 133 to lysine mutation [17,28], was investigated in addition. As seen in Figure 2b, UTI89 Q133K shows no sugar specific interaction and the change in the SPR signal recorded is the same for Au and Au-HM interfaces.


Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion.

Zagorodko O, Bouckaert J, Dumych T, Bilyy R, Larroulet I, Yanguas Serrano A, Alvarez Dorta D, Gouin SG, Dima SO, Oancea F, Boukherroub R, Szunerits S - Biosensors (Basel) (2015)

(a) SPR sensogram of affinity interaction of E. coli UTI (108 cfu/mL) with Au and Au-HM interfaces; flow rate = 20 µL/min; (b) bar graph diagrams of the change in SPR signals of Au and Au-HM interfaces upon interaction for 30 min at a flow rate = 20 µL/min with UTI 89 and UTI89 Q133K.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493549&req=5

biosensors-05-00276-f002: (a) SPR sensogram of affinity interaction of E. coli UTI (108 cfu/mL) with Au and Au-HM interfaces; flow rate = 20 µL/min; (b) bar graph diagrams of the change in SPR signals of Au and Au-HM interfaces upon interaction for 30 min at a flow rate = 20 µL/min with UTI 89 and UTI89 Q133K.
Mentions: The interaction between E. coli (108 cfu/mL) and Au-HM interfaces was investigated in real time using label-free interaction analysis in an SPR assay format (Figure 2). Figure 2a shows the change in the SPR signal over 30 min on Au and Au-HM interfaces upon interaction with E. coli UTI89. The binding ability of E. coli UTI89 to Au-HM was significantly larger than on gold alone, in line with the well-known glycans-mediated increase in pathogen adhesion. To underline the specific aminoheptyl α-D-mannopyranoside-E. coli UTI89 interaction, the adhesion behavior of a strain called UTI89 Q133K, made dysfunctional for mannose binding through a glutamine 133 to lysine mutation [17,28], was investigated in addition. As seen in Figure 2b, UTI89 Q133K shows no sugar specific interaction and the change in the SPR signal recorded is the same for Au and Au-HM interfaces.

Bottom Line: In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner.We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa).This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

View Article: PubMed Central - PubMed

Affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. morjakzzz@gmail.com.

ABSTRACT
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

Show MeSH
Related in: MedlinePlus