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Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion.

Zagorodko O, Bouckaert J, Dumych T, Bilyy R, Larroulet I, Yanguas Serrano A, Alvarez Dorta D, Gouin SG, Dima SO, Oancea F, Boukherroub R, Szunerits S - Biosensors (Basel) (2015)

Bottom Line: In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner.We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa).This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

View Article: PubMed Central - PubMed

Affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. morjakzzz@gmail.com.

ABSTRACT
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

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(a) Reaction scheme for the formation of aminoheptyl α-D-mannopyranoside modified SPR interfaces; (b) C1s core level XPS spectra of Au-COOH and Au-HM interfaces.
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biosensors-05-00276-f001: (a) Reaction scheme for the formation of aminoheptyl α-D-mannopyranoside modified SPR interfaces; (b) C1s core level XPS spectra of Au-COOH and Au-HM interfaces.

Mentions: The covalent linking of HM to gold-based SPR interfaces was achieved in a two-step procedure (Figure 1a). First, an acid-terminated self-assembled monolayer (Au-COOH) was formed through immersion of the gold interface in an ethanolic solution of 11-mercaptoundecanoic acid (MUA) for 24 h. Figure 1B shows the C1s core level photoemission spectrum for the resulting Au-COOH interface. The presence of the aliphatic carbon atoms results in a peak at 285.0 eV, while the feature at 289.1 eV is due to the terminal carboxylic groups.


Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion.

Zagorodko O, Bouckaert J, Dumych T, Bilyy R, Larroulet I, Yanguas Serrano A, Alvarez Dorta D, Gouin SG, Dima SO, Oancea F, Boukherroub R, Szunerits S - Biosensors (Basel) (2015)

(a) Reaction scheme for the formation of aminoheptyl α-D-mannopyranoside modified SPR interfaces; (b) C1s core level XPS spectra of Au-COOH and Au-HM interfaces.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493549&req=5

biosensors-05-00276-f001: (a) Reaction scheme for the formation of aminoheptyl α-D-mannopyranoside modified SPR interfaces; (b) C1s core level XPS spectra of Au-COOH and Au-HM interfaces.
Mentions: The covalent linking of HM to gold-based SPR interfaces was achieved in a two-step procedure (Figure 1a). First, an acid-terminated self-assembled monolayer (Au-COOH) was formed through immersion of the gold interface in an ethanolic solution of 11-mercaptoundecanoic acid (MUA) for 24 h. Figure 1B shows the C1s core level photoemission spectrum for the resulting Au-COOH interface. The presence of the aliphatic carbon atoms results in a peak at 285.0 eV, while the feature at 289.1 eV is due to the terminal carboxylic groups.

Bottom Line: In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner.We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa).This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

View Article: PubMed Central - PubMed

Affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d'Ascq, France. morjakzzz@gmail.com.

ABSTRACT
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

Show MeSH
Related in: MedlinePlus