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Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time.

Kho D, MacDonald C, Johnson R, Unsworth CP, O'Carroll SJ, du Mez E, Angel CE, Graham ES - Biosensors (Basel) (2015)

Bottom Line: In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness.The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay.In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, University of Auckland, Auckland 1023, New Zealand. s.ocarroll@auckland.ac.nz.

ABSTRACT
The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.

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Optimisation of ideal culture conditions. Different Cell Index adhesion curves are obtained from different cells types. The adhesion of (a) HMEC-1 endothelial cells and (b) NTera2/D1 (NT2)-astrocytes is shown. Both cell types were seeded at a range of titrations to identify the ideal seeding density and reveal time windows for drug treatment. Curves represent the mean Cell Index value from >4 wells ± SD.
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biosensors-05-00199-f003: Optimisation of ideal culture conditions. Different Cell Index adhesion curves are obtained from different cells types. The adhesion of (a) HMEC-1 endothelial cells and (b) NTera2/D1 (NT2)-astrocytes is shown. Both cell types were seeded at a range of titrations to identify the ideal seeding density and reveal time windows for drug treatment. Curves represent the mean Cell Index value from >4 wells ± SD.

Mentions: Defining the ideal seeding density and optimal culture conditions using the xCELLigence biosensor: Defining the appropriate seeding density and window of culture are very important aspects of the experimental design. Figure 3 shows examples of growth curves obtained from two quite different cell types, (1) endothelial HMEC-1 cells and (3) NT2-astrocytes, which have been grown at different seeding densities for six days to identify the ideal culture conditions.


Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time.

Kho D, MacDonald C, Johnson R, Unsworth CP, O'Carroll SJ, du Mez E, Angel CE, Graham ES - Biosensors (Basel) (2015)

Optimisation of ideal culture conditions. Different Cell Index adhesion curves are obtained from different cells types. The adhesion of (a) HMEC-1 endothelial cells and (b) NTera2/D1 (NT2)-astrocytes is shown. Both cell types were seeded at a range of titrations to identify the ideal seeding density and reveal time windows for drug treatment. Curves represent the mean Cell Index value from >4 wells ± SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493546&req=5

biosensors-05-00199-f003: Optimisation of ideal culture conditions. Different Cell Index adhesion curves are obtained from different cells types. The adhesion of (a) HMEC-1 endothelial cells and (b) NTera2/D1 (NT2)-astrocytes is shown. Both cell types were seeded at a range of titrations to identify the ideal seeding density and reveal time windows for drug treatment. Curves represent the mean Cell Index value from >4 wells ± SD.
Mentions: Defining the ideal seeding density and optimal culture conditions using the xCELLigence biosensor: Defining the appropriate seeding density and window of culture are very important aspects of the experimental design. Figure 3 shows examples of growth curves obtained from two quite different cell types, (1) endothelial HMEC-1 cells and (3) NT2-astrocytes, which have been grown at different seeding densities for six days to identify the ideal culture conditions.

Bottom Line: In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness.The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay.In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, University of Auckland, Auckland 1023, New Zealand. s.ocarroll@auckland.ac.nz.

ABSTRACT
The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.

Show MeSH
Related in: MedlinePlus