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CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus

Biodistribution of nanoprobes in organs of tumor-bearing mice after 4 h and 24 h intravenous injection with 150 μL GNS-PEG-CD44v6 and GNS-PEG respectively (0.867 mg Au/mL). Error bars were based on triplet measurements.
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Figure 8: Biodistribution of nanoprobes in organs of tumor-bearing mice after 4 h and 24 h intravenous injection with 150 μL GNS-PEG-CD44v6 and GNS-PEG respectively (0.867 mg Au/mL). Error bars were based on triplet measurements.

Mentions: To further quantify the PA signals of the tumor site, an identical region of interest (ROI) was selected in each MIP image, which is indicated by a white dashed circle in the first image of Fig. 7D. The trend of the normalized PA signal enhancement over time of the five groups was illustrated (Supplementary Fig. S7). The PA signal reached a peak at 4 h after injection in the five groups, followed by a decrease at 24 h. In the subcutaneous GC xenografted model, the PA enhancements of the GNS-PEG-CD44v6 group were 4.7-fold higher at 4 h after injection, compared with 2.5-fold higher in GNS-PEG-CD44 group. In the orthotopic xenografted model, slight enhancements were observed. In contrast, the PA enhancements were 1.75-fold higher at 4 h after injection in GNS-PEG injected group. Overall, at 4 h post-injection, enhancement of the GNS-PEG-CD44v6 group was on average 250% times greater than that with PEG-GNS (p < 0.05), which correlated well with the Au distribution in ICP-MS results, shown in Fig. 8. The distinct difference demonstrated the targeting delivery ability of GNS-PEG-CD44v6 to tumors.


CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Biodistribution of nanoprobes in organs of tumor-bearing mice after 4 h and 24 h intravenous injection with 150 μL GNS-PEG-CD44v6 and GNS-PEG respectively (0.867 mg Au/mL). Error bars were based on triplet measurements.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493535&req=5

Figure 8: Biodistribution of nanoprobes in organs of tumor-bearing mice after 4 h and 24 h intravenous injection with 150 μL GNS-PEG-CD44v6 and GNS-PEG respectively (0.867 mg Au/mL). Error bars were based on triplet measurements.
Mentions: To further quantify the PA signals of the tumor site, an identical region of interest (ROI) was selected in each MIP image, which is indicated by a white dashed circle in the first image of Fig. 7D. The trend of the normalized PA signal enhancement over time of the five groups was illustrated (Supplementary Fig. S7). The PA signal reached a peak at 4 h after injection in the five groups, followed by a decrease at 24 h. In the subcutaneous GC xenografted model, the PA enhancements of the GNS-PEG-CD44v6 group were 4.7-fold higher at 4 h after injection, compared with 2.5-fold higher in GNS-PEG-CD44 group. In the orthotopic xenografted model, slight enhancements were observed. In contrast, the PA enhancements were 1.75-fold higher at 4 h after injection in GNS-PEG injected group. Overall, at 4 h post-injection, enhancement of the GNS-PEG-CD44v6 group was on average 250% times greater than that with PEG-GNS (p < 0.05), which correlated well with the Au distribution in ICP-MS results, shown in Fig. 8. The distinct difference demonstrated the targeting delivery ability of GNS-PEG-CD44v6 to tumors.

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus