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CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus

Viability of GCSCs (CD44+sphere cells) (A) and GES-1 cells (B) treated with GNS-PEG or CD44-targetable GNS-PEG-CD44v6 measured by MTT assay; TEM images of GCSCs treated with GNS-PEG (C, F) and GNS-PEG-CD44v6 (D, G); The arrow indicates that the nannoparticles are mainly gathered in the cytoplasm and nucleus cleft of CD44+sphere cells (D, G) and GC cells (E, H).
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Figure 4: Viability of GCSCs (CD44+sphere cells) (A) and GES-1 cells (B) treated with GNS-PEG or CD44-targetable GNS-PEG-CD44v6 measured by MTT assay; TEM images of GCSCs treated with GNS-PEG (C, F) and GNS-PEG-CD44v6 (D, G); The arrow indicates that the nannoparticles are mainly gathered in the cytoplasm and nucleus cleft of CD44+sphere cells (D, G) and GC cells (E, H).

Mentions: A standard MTT colorimetric assay was performed to assess the potential deleterious effect of GNS-PEG and GNS-PEG-CD44v6 towards GES-1 and GCSCs after co-incubation for 24 h. Both GNS-PEG and GNS-PEG-CD44v6 exhibited low cytotoxicity at doses up to 200 μg/mL (Fig. 4A-B). The excellent biocompatibility was due to the CTAB-free green synthesis of GNSs and the encapsulation of PEG.


CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Viability of GCSCs (CD44+sphere cells) (A) and GES-1 cells (B) treated with GNS-PEG or CD44-targetable GNS-PEG-CD44v6 measured by MTT assay; TEM images of GCSCs treated with GNS-PEG (C, F) and GNS-PEG-CD44v6 (D, G); The arrow indicates that the nannoparticles are mainly gathered in the cytoplasm and nucleus cleft of CD44+sphere cells (D, G) and GC cells (E, H).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493535&req=5

Figure 4: Viability of GCSCs (CD44+sphere cells) (A) and GES-1 cells (B) treated with GNS-PEG or CD44-targetable GNS-PEG-CD44v6 measured by MTT assay; TEM images of GCSCs treated with GNS-PEG (C, F) and GNS-PEG-CD44v6 (D, G); The arrow indicates that the nannoparticles are mainly gathered in the cytoplasm and nucleus cleft of CD44+sphere cells (D, G) and GC cells (E, H).
Mentions: A standard MTT colorimetric assay was performed to assess the potential deleterious effect of GNS-PEG and GNS-PEG-CD44v6 towards GES-1 and GCSCs after co-incubation for 24 h. Both GNS-PEG and GNS-PEG-CD44v6 exhibited low cytotoxicity at doses up to 200 μg/mL (Fig. 4A-B). The excellent biocompatibility was due to the CTAB-free green synthesis of GNSs and the encapsulation of PEG.

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus