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CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus

Characterization of plasmonic-resonant GNSs: (A and B) The average hydrodynamic diameter of GNS-PEG and GNS-PEG-CD44v6; (C) UV-Vis-NIR spectra of GNSs (black line), GNS-PEG (blue line) and GNS-PEG-CD44v6 solution (red line), with an absorption peak at 846 nm; (D) The EDX spectroscopy of the GNS-PEG-CD44v6 by TEM.
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Figure 2: Characterization of plasmonic-resonant GNSs: (A and B) The average hydrodynamic diameter of GNS-PEG and GNS-PEG-CD44v6; (C) UV-Vis-NIR spectra of GNSs (black line), GNS-PEG (blue line) and GNS-PEG-CD44v6 solution (red line), with an absorption peak at 846 nm; (D) The EDX spectroscopy of the GNS-PEG-CD44v6 by TEM.

Mentions: In this study, anti-CD44 and anti-CD44v6 monoclonal antibodies were conjugated to the multi-branched GNSs to evaluate the specific cellular affinity between the nanoprobes and GCSCs. The coupling rates of anti-CD44/CD44v6 monoclonal antibodies were 71.9% and 72.7%, respectively. The GNSs showed a plasmon absorption band at 833 nm, while the absorption peak exhibited a slight red shift to 858 nm and 846 nm after encapsulating with PEG and coupling with antibodies (Fig. 2C). The naked GNSs became agglomerate soon and difficult to resuspend after centrifugation, without encapsulated with PEG. Therefore, the dynamic light scattering (DLS) measurement was hardly possible. The average hydrodynamic diameters of GNS-PEG and GNS-PEG-CD44v6 were 89.5 nm and 92.1 nm, respectively (Fig. 2A-B). The ζ-potential increased from -46.9 mV (GNS-PEG) to -12.3 mV (GNS-PEG-CD44v6). The EDX spectroscopy of the GNS-PEG-CD44v6 is shown in Fig. 2D, indicating that PEG had been connected to the GNSs.


CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Characterization of plasmonic-resonant GNSs: (A and B) The average hydrodynamic diameter of GNS-PEG and GNS-PEG-CD44v6; (C) UV-Vis-NIR spectra of GNSs (black line), GNS-PEG (blue line) and GNS-PEG-CD44v6 solution (red line), with an absorption peak at 846 nm; (D) The EDX spectroscopy of the GNS-PEG-CD44v6 by TEM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493535&req=5

Figure 2: Characterization of plasmonic-resonant GNSs: (A and B) The average hydrodynamic diameter of GNS-PEG and GNS-PEG-CD44v6; (C) UV-Vis-NIR spectra of GNSs (black line), GNS-PEG (blue line) and GNS-PEG-CD44v6 solution (red line), with an absorption peak at 846 nm; (D) The EDX spectroscopy of the GNS-PEG-CD44v6 by TEM.
Mentions: In this study, anti-CD44 and anti-CD44v6 monoclonal antibodies were conjugated to the multi-branched GNSs to evaluate the specific cellular affinity between the nanoprobes and GCSCs. The coupling rates of anti-CD44/CD44v6 monoclonal antibodies were 71.9% and 72.7%, respectively. The GNSs showed a plasmon absorption band at 833 nm, while the absorption peak exhibited a slight red shift to 858 nm and 846 nm after encapsulating with PEG and coupling with antibodies (Fig. 2C). The naked GNSs became agglomerate soon and difficult to resuspend after centrifugation, without encapsulated with PEG. Therefore, the dynamic light scattering (DLS) measurement was hardly possible. The average hydrodynamic diameters of GNS-PEG and GNS-PEG-CD44v6 were 89.5 nm and 92.1 nm, respectively (Fig. 2A-B). The ζ-potential increased from -46.9 mV (GNS-PEG) to -12.3 mV (GNS-PEG-CD44v6). The EDX spectroscopy of the GNS-PEG-CD44v6 is shown in Fig. 2D, indicating that PEG had been connected to the GNSs.

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus