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CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus

Representative FESEM (A) and TEM (B and E) images of GNSs; (C) HRTEM images of GNS branchand lattice fringes on a single branchtip; (D) FESEM image of PEG-encapsulated GNSs; (F) Diffraction spectrum image of GNS.
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Figure 1: Representative FESEM (A) and TEM (B and E) images of GNSs; (C) HRTEM images of GNS branchand lattice fringes on a single branchtip; (D) FESEM image of PEG-encapsulated GNSs; (F) Diffraction spectrum image of GNS.

Mentions: The GNS-based PEGylated and CD44v6 antibody-conjugated multifunctional nanoprobes (GNS-PEG-CD44v6) were prepared for targeted PA, X-ray/CT imaging (Supplementary Fig. S2 and Supplementary Fig. S3) and photothermal therapy of GCSCs (Scheme 1). The GNSs were stabilized and chemically functionalized by heterobifunctional polyethyleneglycol (Carboxymethyl-PEG-SH) 38, which could bind with the gold nanoparticles by the oxidative addition of the S-H and connect to the antibodies by the carboxyl group. The nanoprobes were characterized with UV-Vis absorption spectroscopy, FESEM (Fig. 1A and Fig. 1D) and TEM (Fig. 1B and Fig. 1E). The peak position of the surface plasmon resonance (SPR) of GNSs was tunable by the width of base, the diameters of core and tips, the numbers of branches and aspect ratios 35, 39. The SPR peaks could be shifted to the NIR region by increasing the number of branches. The products synthesized in our study were branched nanoparticles with more than eight branches, in contrast to 3 to 6, with the absorption band located at about 635 nm 40. The high-resolution TEM (HRTEM) image of a single branch (Fig. 1C) of GNSs showed lattice fringes with an average interface distance of 0.22 nm. The selected area electron diffraction (SAED) pattern (Fig. 1F) and lattice fringes of the GNS confirmed that each of the GNSs was multicrystalline.


CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and Plasmonic Photothermal Therapy of Gastric Cancer Stem-like Cells.

Liang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D - Theranostics (2015)

Representative FESEM (A) and TEM (B and E) images of GNSs; (C) HRTEM images of GNS branchand lattice fringes on a single branchtip; (D) FESEM image of PEG-encapsulated GNSs; (F) Diffraction spectrum image of GNS.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493535&req=5

Figure 1: Representative FESEM (A) and TEM (B and E) images of GNSs; (C) HRTEM images of GNS branchand lattice fringes on a single branchtip; (D) FESEM image of PEG-encapsulated GNSs; (F) Diffraction spectrum image of GNS.
Mentions: The GNS-based PEGylated and CD44v6 antibody-conjugated multifunctional nanoprobes (GNS-PEG-CD44v6) were prepared for targeted PA, X-ray/CT imaging (Supplementary Fig. S2 and Supplementary Fig. S3) and photothermal therapy of GCSCs (Scheme 1). The GNSs were stabilized and chemically functionalized by heterobifunctional polyethyleneglycol (Carboxymethyl-PEG-SH) 38, which could bind with the gold nanoparticles by the oxidative addition of the S-H and connect to the antibodies by the carboxyl group. The nanoprobes were characterized with UV-Vis absorption spectroscopy, FESEM (Fig. 1A and Fig. 1D) and TEM (Fig. 1B and Fig. 1E). The peak position of the surface plasmon resonance (SPR) of GNSs was tunable by the width of base, the diameters of core and tips, the numbers of branches and aspect ratios 35, 39. The SPR peaks could be shifted to the NIR region by increasing the number of branches. The products synthesized in our study were branched nanoparticles with more than eight branches, in contrast to 3 to 6, with the absorption band located at about 635 nm 40. The high-resolution TEM (HRTEM) image of a single branch (Fig. 1C) of GNSs showed lattice fringes with an average interface distance of 0.22 nm. The selected area electron diffraction (SAED) pattern (Fig. 1F) and lattice fringes of the GNS confirmed that each of the GNSs was multicrystalline.

Bottom Line: Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies.It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment.Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed.

View Article: PubMed Central - PubMed

Affiliation: 1. Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P. R. China ; 2. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Instrument Science and Engineering, Research Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

ABSTRACT
Developing safe and effective nanoprobes for targeted imaging and selective therapy of gastric cancer stem cells (GCSCs) has become one of the most promising anticancer strategies. Herein, gold nanostars-based PEGylated multifunctional nanoprobes were prepared with conjugated CD44v6 monoclonal antibodies (CD44v6-GNS) as the targeting ligands. It was observed that the prepared nanoprobes had high affinity towards GCSC spheroid colonies and destroyed them completely with a low power density upon near-infrared (NIR) laser treatment (790 nm, 1.5 W/cm(2), 5 min) in vitro experiment. Orthotopic and subcutaneous xenografted nude mice models of human gastric cancer were established. Subsequently, biodistribution and photothermal therapeutic effects after being intravenously injected with the prepared nanoprobes were assessed. Photoacoustic imaging revealed that CD44v6-GNS nanoprobes could target the gastric cancer vascular system actively at 4 h post-injection, while the probes inhibited tumor growth remarkably upon NIR laser irradiation, and even extended survivability of the gastric cancer-bearing mice. The CD44v6-GNS nanoprobes exhibited great potential for applications of gastric cancer targeted imaging and photothermal therapy in the near future.

No MeSH data available.


Related in: MedlinePlus