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A Plasmonic Gold Nanostar Theranostic Probe for In Vivo Tumor Imaging and Photothermal Therapy.

Liu Y, Ashton JR, Moding EJ, Yuan H, Register JK, Fales AM, Choi J, Whitley MJ, Zhao X, Qi Y, Ma Y, Vaidyanathan G, Zalutsky MR, Kirsch DG, Badea CT, Vo-Dinh T - Theranostics (2015)

Bottom Line: Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity.We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice.In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS.

View Article: PubMed Central - PubMed

Affiliation: 1. Fitzpatrick Institute for Photonics, Duke University, Durham, NC, 27708, United States ; 2. Department of Biomedical Engineering, Duke University, Durham, NC, 27708, United States ; 3. Department of Chemistry, Duke University, Durham, NC, 27708, United States.

ABSTRACT
Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity. In this study, we developed a gold nanostar (GNS) probe for multi-modality theranostics including surface-enhanced Raman scattering (SERS) detection, x-ray computed tomography (CT), two-photon luminescence (TPL) imaging, and photothermal therapy (PTT). We performed radiolabeling, as well as CT and optical imaging, to investigate the GNS probe's biodistribution and intratumoral uptake at both macroscopic and microscopic scales. We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice. The results showed that 30-nm GNS have higher tumor uptake, as well as deeper penetration into tumor interstitial space compared to 60-nm GNS. In addition, we found that a higher injection dose of GNS can increase the percentage of tumor uptake. We also demonstrated the GNS probe's superior photothermal conversion efficiency with a highly concentrated heating effect due to a tip-enhanced plasmonic effect. In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS. This multifunctional GNS probe has the potential to be used for in vivo biosensing, preoperative CT imaging, intraoperative detection with optical methods (SERS and TPL), as well as image-guided photothermal therapy.

No MeSH data available.


Related in: MedlinePlus

Photographs (top) and x-ray images (bottom) of mice before and after photothermal therapy with tumors circled in red. The control mouse images were taken 7 days after treatment and the images of the mouse with GNS injection were taken 3 days after treatment. Dark discoloration in the tumor region for the GNS mouse is due to nanoparticle accumulation in the underlying tumor. X-ray images show a clear decrease in tumor bulk for the mouse with GNS injection, but a significant increase in tumor size for the mouse with PBS injection. Similar results were obtained for the second mouse tested in each group.
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Figure 9: Photographs (top) and x-ray images (bottom) of mice before and after photothermal therapy with tumors circled in red. The control mouse images were taken 7 days after treatment and the images of the mouse with GNS injection were taken 3 days after treatment. Dark discoloration in the tumor region for the GNS mouse is due to nanoparticle accumulation in the underlying tumor. X-ray images show a clear decrease in tumor bulk for the mouse with GNS injection, but a significant increase in tumor size for the mouse with PBS injection. Similar results were obtained for the second mouse tested in each group.

Mentions: Following in vitro evaluation, we performed in vivo photothermal therapy on mice bearing primary sarcomas. Figure 8 shows NIR images depicting surface temperature change during the photothermal treatment process and the measured temperature increase of sarcomas with and without GNS over 10 minutes of laser exposure. The tumor temperature is much higher for mice with GNS injection than for mice with PBS injection. The tumor surface temperature with GNS reaches 50 °C after only 4 minutes of treatment, which is high enough to kill tumor cells.51 The tumors treated with GNS and laser irradiation regressed to an undetectable size one day after photothermal therapy with GNS, while tumors treated with PBS and laser irradiation continued to grow rapidly. Photographs and x-ray images of these two conditions are shown in Figure 9. The GNS mice exhibited no sign of tumor growth for 7 days following treatment, at which point they were sacrificed. The laser irradiation was confined primarily to the tumor and there was no detectable tissue damage outside of the tumor region. There was some skin burning directly over the tumor surface, but no other adverse effects were observed in these mice. These results provide the proof of principle that the GNS nanoprobe may provide a useful platform for image-guided photothermal therapy, which will be investigated further in future pre-clinical experiments.


A Plasmonic Gold Nanostar Theranostic Probe for In Vivo Tumor Imaging and Photothermal Therapy.

Liu Y, Ashton JR, Moding EJ, Yuan H, Register JK, Fales AM, Choi J, Whitley MJ, Zhao X, Qi Y, Ma Y, Vaidyanathan G, Zalutsky MR, Kirsch DG, Badea CT, Vo-Dinh T - Theranostics (2015)

Photographs (top) and x-ray images (bottom) of mice before and after photothermal therapy with tumors circled in red. The control mouse images were taken 7 days after treatment and the images of the mouse with GNS injection were taken 3 days after treatment. Dark discoloration in the tumor region for the GNS mouse is due to nanoparticle accumulation in the underlying tumor. X-ray images show a clear decrease in tumor bulk for the mouse with GNS injection, but a significant increase in tumor size for the mouse with PBS injection. Similar results were obtained for the second mouse tested in each group.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4493533&req=5

Figure 9: Photographs (top) and x-ray images (bottom) of mice before and after photothermal therapy with tumors circled in red. The control mouse images were taken 7 days after treatment and the images of the mouse with GNS injection were taken 3 days after treatment. Dark discoloration in the tumor region for the GNS mouse is due to nanoparticle accumulation in the underlying tumor. X-ray images show a clear decrease in tumor bulk for the mouse with GNS injection, but a significant increase in tumor size for the mouse with PBS injection. Similar results were obtained for the second mouse tested in each group.
Mentions: Following in vitro evaluation, we performed in vivo photothermal therapy on mice bearing primary sarcomas. Figure 8 shows NIR images depicting surface temperature change during the photothermal treatment process and the measured temperature increase of sarcomas with and without GNS over 10 minutes of laser exposure. The tumor temperature is much higher for mice with GNS injection than for mice with PBS injection. The tumor surface temperature with GNS reaches 50 °C after only 4 minutes of treatment, which is high enough to kill tumor cells.51 The tumors treated with GNS and laser irradiation regressed to an undetectable size one day after photothermal therapy with GNS, while tumors treated with PBS and laser irradiation continued to grow rapidly. Photographs and x-ray images of these two conditions are shown in Figure 9. The GNS mice exhibited no sign of tumor growth for 7 days following treatment, at which point they were sacrificed. The laser irradiation was confined primarily to the tumor and there was no detectable tissue damage outside of the tumor region. There was some skin burning directly over the tumor surface, but no other adverse effects were observed in these mice. These results provide the proof of principle that the GNS nanoprobe may provide a useful platform for image-guided photothermal therapy, which will be investigated further in future pre-clinical experiments.

Bottom Line: Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity.We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice.In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS.

View Article: PubMed Central - PubMed

Affiliation: 1. Fitzpatrick Institute for Photonics, Duke University, Durham, NC, 27708, United States ; 2. Department of Biomedical Engineering, Duke University, Durham, NC, 27708, United States ; 3. Department of Chemistry, Duke University, Durham, NC, 27708, United States.

ABSTRACT
Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity. In this study, we developed a gold nanostar (GNS) probe for multi-modality theranostics including surface-enhanced Raman scattering (SERS) detection, x-ray computed tomography (CT), two-photon luminescence (TPL) imaging, and photothermal therapy (PTT). We performed radiolabeling, as well as CT and optical imaging, to investigate the GNS probe's biodistribution and intratumoral uptake at both macroscopic and microscopic scales. We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice. The results showed that 30-nm GNS have higher tumor uptake, as well as deeper penetration into tumor interstitial space compared to 60-nm GNS. In addition, we found that a higher injection dose of GNS can increase the percentage of tumor uptake. We also demonstrated the GNS probe's superior photothermal conversion efficiency with a highly concentrated heating effect due to a tip-enhanced plasmonic effect. In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS. This multifunctional GNS probe has the potential to be used for in vivo biosensing, preoperative CT imaging, intraoperative detection with optical methods (SERS and TPL), as well as image-guided photothermal therapy.

No MeSH data available.


Related in: MedlinePlus