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A Plasmonic Gold Nanostar Theranostic Probe for In Vivo Tumor Imaging and Photothermal Therapy.

Liu Y, Ashton JR, Moding EJ, Yuan H, Register JK, Fales AM, Choi J, Whitley MJ, Zhao X, Qi Y, Ma Y, Vaidyanathan G, Zalutsky MR, Kirsch DG, Badea CT, Vo-Dinh T - Theranostics (2015)

Bottom Line: Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity.We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice.In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS.

View Article: PubMed Central - PubMed

Affiliation: 1. Fitzpatrick Institute for Photonics, Duke University, Durham, NC, 27708, United States ; 2. Department of Biomedical Engineering, Duke University, Durham, NC, 27708, United States ; 3. Department of Chemistry, Duke University, Durham, NC, 27708, United States.

ABSTRACT
Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity. In this study, we developed a gold nanostar (GNS) probe for multi-modality theranostics including surface-enhanced Raman scattering (SERS) detection, x-ray computed tomography (CT), two-photon luminescence (TPL) imaging, and photothermal therapy (PTT). We performed radiolabeling, as well as CT and optical imaging, to investigate the GNS probe's biodistribution and intratumoral uptake at both macroscopic and microscopic scales. We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice. The results showed that 30-nm GNS have higher tumor uptake, as well as deeper penetration into tumor interstitial space compared to 60-nm GNS. In addition, we found that a higher injection dose of GNS can increase the percentage of tumor uptake. We also demonstrated the GNS probe's superior photothermal conversion efficiency with a highly concentrated heating effect due to a tip-enhanced plasmonic effect. In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS. This multifunctional GNS probe has the potential to be used for in vivo biosensing, preoperative CT imaging, intraoperative detection with optical methods (SERS and TPL), as well as image-guided photothermal therapy.

No MeSH data available.


Related in: MedlinePlus

Biodistribution (%ID/g) of GNS with different particle sizes and injection doses 48 hours after tail vein injection. Four mice with xenograft sarcomas were used for each group. Smaller nanostars with higher injection dose have higher tumor uptake. The %ID/g is defined as percentage of total injection dose per gram tissue weight. Error bars show standard deviation. The asterisk represents a statistically significant difference from the other two groups (p<0.05).
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Figure 2: Biodistribution (%ID/g) of GNS with different particle sizes and injection doses 48 hours after tail vein injection. Four mice with xenograft sarcomas were used for each group. Smaller nanostars with higher injection dose have higher tumor uptake. The %ID/g is defined as percentage of total injection dose per gram tissue weight. Error bars show standard deviation. The asterisk represents a statistically significant difference from the other two groups (p<0.05).

Mentions: Radiolabelled GNS (30 nm and 60 nm) were injected intravenously into mice with xenograft sarcomas, and radioactivity uptake in tumor and other organs of interest was measured after 48 hours; results are shown in Figure 2. Tumor uptake of 30-nm GNS was higher (2.11 ± 0.64 %ID/g) than that of 60-nm GNS (0.88 ± 0.46 %ID/g) with the same injection dose of gold (200 μg). In addition, 30-nm GNS showed a higher tumor uptake with a 200-μg injection dose (2.11 ± 0.64 %ID/g) than with 50-μg injection dose (1.08 ± 0.25 %ID/g). This difference was found to be statistically significant. The reticuloendothelial system (RES), including liver and spleen, demonstrated high uptake of nanoparticles, which is consistent with previous studies.43 Relatively low uptake levels of nanoparticles were seen in other non-target organs.


A Plasmonic Gold Nanostar Theranostic Probe for In Vivo Tumor Imaging and Photothermal Therapy.

Liu Y, Ashton JR, Moding EJ, Yuan H, Register JK, Fales AM, Choi J, Whitley MJ, Zhao X, Qi Y, Ma Y, Vaidyanathan G, Zalutsky MR, Kirsch DG, Badea CT, Vo-Dinh T - Theranostics (2015)

Biodistribution (%ID/g) of GNS with different particle sizes and injection doses 48 hours after tail vein injection. Four mice with xenograft sarcomas were used for each group. Smaller nanostars with higher injection dose have higher tumor uptake. The %ID/g is defined as percentage of total injection dose per gram tissue weight. Error bars show standard deviation. The asterisk represents a statistically significant difference from the other two groups (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4493533&req=5

Figure 2: Biodistribution (%ID/g) of GNS with different particle sizes and injection doses 48 hours after tail vein injection. Four mice with xenograft sarcomas were used for each group. Smaller nanostars with higher injection dose have higher tumor uptake. The %ID/g is defined as percentage of total injection dose per gram tissue weight. Error bars show standard deviation. The asterisk represents a statistically significant difference from the other two groups (p<0.05).
Mentions: Radiolabelled GNS (30 nm and 60 nm) were injected intravenously into mice with xenograft sarcomas, and radioactivity uptake in tumor and other organs of interest was measured after 48 hours; results are shown in Figure 2. Tumor uptake of 30-nm GNS was higher (2.11 ± 0.64 %ID/g) than that of 60-nm GNS (0.88 ± 0.46 %ID/g) with the same injection dose of gold (200 μg). In addition, 30-nm GNS showed a higher tumor uptake with a 200-μg injection dose (2.11 ± 0.64 %ID/g) than with 50-μg injection dose (1.08 ± 0.25 %ID/g). This difference was found to be statistically significant. The reticuloendothelial system (RES), including liver and spleen, demonstrated high uptake of nanoparticles, which is consistent with previous studies.43 Relatively low uptake levels of nanoparticles were seen in other non-target organs.

Bottom Line: Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity.We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice.In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS.

View Article: PubMed Central - PubMed

Affiliation: 1. Fitzpatrick Institute for Photonics, Duke University, Durham, NC, 27708, United States ; 2. Department of Biomedical Engineering, Duke University, Durham, NC, 27708, United States ; 3. Department of Chemistry, Duke University, Durham, NC, 27708, United States.

ABSTRACT
Nanomedicine has attracted increasing attention in recent years, because it offers great promise to provide personalized diagnostics and therapy with improved treatment efficacy and specificity. In this study, we developed a gold nanostar (GNS) probe for multi-modality theranostics including surface-enhanced Raman scattering (SERS) detection, x-ray computed tomography (CT), two-photon luminescence (TPL) imaging, and photothermal therapy (PTT). We performed radiolabeling, as well as CT and optical imaging, to investigate the GNS probe's biodistribution and intratumoral uptake at both macroscopic and microscopic scales. We also characterized the performance of the GNS nanoprobe for in vitro photothermal heating and in vivo photothermal ablation of primary sarcomas in mice. The results showed that 30-nm GNS have higher tumor uptake, as well as deeper penetration into tumor interstitial space compared to 60-nm GNS. In addition, we found that a higher injection dose of GNS can increase the percentage of tumor uptake. We also demonstrated the GNS probe's superior photothermal conversion efficiency with a highly concentrated heating effect due to a tip-enhanced plasmonic effect. In vivo photothermal therapy with a near-infrared (NIR) laser under the maximum permissible exposure (MPE) led to ablation of aggressive tumors containing GNS, but had no effect in the absence of GNS. This multifunctional GNS probe has the potential to be used for in vivo biosensing, preoperative CT imaging, intraoperative detection with optical methods (SERS and TPL), as well as image-guided photothermal therapy.

No MeSH data available.


Related in: MedlinePlus