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Smart MoS2/Fe3O4 Nanotheranostic for Magnetically Targeted Photothermal Therapy Guided by Magnetic Resonance/Photoacoustic Imaging.

Yu J, Yin W, Zheng X, Tian G, Zhang X, Bao T, Dong X, Wang Z, Gu Z, Ma X, Zhao Y - Theranostics (2015)

Bottom Line: The MoS2/Fe3O4 composite (MSIOs) functionalized by biocompatible polyethylene glycol (PEG) were prepared by a simple two-step hydrothermal method.And the as-obtained MSIOs exhibit high stability in bio-fluids and low toxicity in vitro and in vivo.Specifically, the MSIOs can be applied as a dual-modal probe for T2-weighted magnetic resonance (MR) and photoacoustic tomography (PAT) imaging due to their superparamagnetic property and strong NIR absorption.

View Article: PubMed Central - PubMed

Affiliation: 1. Key Laboratory of Polymer Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, Shaanxi, China ; 2. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Beijing, China.

ABSTRACT
The ability to selectively destroy cancer cells while sparing normal tissue is highly desirable during the cancer therapy. Here, magnetic targeted photothermal therapy was demonstrated by the integration of MoS2 (MS) flakes and Fe3O4 (IO) nanoparticles (NPs), where MoS2 converted near-infrared (NIR) light into heat and Fe3O4 NPs served as target moiety directed by external magnetic field to tumor site. The MoS2/Fe3O4 composite (MSIOs) functionalized by biocompatible polyethylene glycol (PEG) were prepared by a simple two-step hydrothermal method. And the as-obtained MSIOs exhibit high stability in bio-fluids and low toxicity in vitro and in vivo. Specifically, the MSIOs can be applied as a dual-modal probe for T2-weighted magnetic resonance (MR) and photoacoustic tomography (PAT) imaging due to their superparamagnetic property and strong NIR absorption. Furthermore, we demonstrate an effective result for magnetically targeted photothermal ablation of cancer. All these results show a great potential for localized photothermal ablation of cancer spatially/timely guided by the magnetic field and indicated the promise of the multifunctional MSIOs for applications in cancer theranostics.

No MeSH data available.


Related in: MedlinePlus

(a) H&E-stained histological slices from mice receiving no injection (control) and (b) post-injected with the MSIOs for 7 day. (b) Biodistribution of the MSIOs in different time intervals after injection of MSIOs determined by ICP-MS measurements of Mo element in tissue lysates. (c) Body weights change of the post-injected mice within 15 day compared with the control group without injection of MSIOs. Scale bars in (a) is 100 μm.
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Figure 6: (a) H&E-stained histological slices from mice receiving no injection (control) and (b) post-injected with the MSIOs for 7 day. (b) Biodistribution of the MSIOs in different time intervals after injection of MSIOs determined by ICP-MS measurements of Mo element in tissue lysates. (c) Body weights change of the post-injected mice within 15 day compared with the control group without injection of MSIOs. Scale bars in (a) is 100 μm.

Mentions: For the further use of MSIOs as a magnetic targeting PTT agent in vivo, it is necessary to understand the potential toxicology of the MSIOs. Therefore, the in vivo toxicity assessments of the MSIOs including the histological sliced analysis, biodistribution and body weight, were conducted. BALB/C tumor-bearing mice were i.v. injected with a dose of MSIOs of 20 mg/kg of body weight (200 μL, 2 mg/mL). Compared with the control group, the histological examination of hematoxylin and eosin (H&E) stained slices of the major organs such as heart, liver, spleen, lung and kidney showed that there were no noticeable organ damage and inflammatory lesion for all the major organs of the mice after injection with MSIOs for 7 days (Figure 6a). Also, no necrosis was found in any of the groups. The biodistribution results in Figure 6b detected by ICP-MS indicated that the early high concentration accumulation of MSIOs in the liver and spleen was expected which was related to the clearance of foreign materials by the macrophage system within 24 h 34. At the 48 h time point, there was a slight decrease in the amount of the MSIOs in the liver and spleen, suggesting the gradually clearance from the two organs. After 7 day, notable decreases of the MSIOs in the liver and spleen were found, indicating that the MSIOs have an overt clearance from the two organs. Also, a relatively high accumulation of the MSIOs within tumor can be observed within 24 h and gradually clearance from the tumor at the 48 h and 7 day. A combined effect of a locally increased capillary permeability at the tumor site due to the EPR effect and the strong magnetic targeting effect may be responsible for the increased accumulation of MSIOs within 24 h, which was proved by the ICP-MS result (Supplementary Figure S10). Meanwhile, fluctuation of body weight is a useful indicator for investigating the toxicity effect of the MSIOs. No significant body weight loss was observed in the treated group over a period of 15 days (Figure 6c). These results proved that MSIOs at the given dose hardly cause in vivo toxicity and could be used as an injectable theranostic agent in vivo.


Smart MoS2/Fe3O4 Nanotheranostic for Magnetically Targeted Photothermal Therapy Guided by Magnetic Resonance/Photoacoustic Imaging.

Yu J, Yin W, Zheng X, Tian G, Zhang X, Bao T, Dong X, Wang Z, Gu Z, Ma X, Zhao Y - Theranostics (2015)

(a) H&E-stained histological slices from mice receiving no injection (control) and (b) post-injected with the MSIOs for 7 day. (b) Biodistribution of the MSIOs in different time intervals after injection of MSIOs determined by ICP-MS measurements of Mo element in tissue lysates. (c) Body weights change of the post-injected mice within 15 day compared with the control group without injection of MSIOs. Scale bars in (a) is 100 μm.
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Related In: Results  -  Collection

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Figure 6: (a) H&E-stained histological slices from mice receiving no injection (control) and (b) post-injected with the MSIOs for 7 day. (b) Biodistribution of the MSIOs in different time intervals after injection of MSIOs determined by ICP-MS measurements of Mo element in tissue lysates. (c) Body weights change of the post-injected mice within 15 day compared with the control group without injection of MSIOs. Scale bars in (a) is 100 μm.
Mentions: For the further use of MSIOs as a magnetic targeting PTT agent in vivo, it is necessary to understand the potential toxicology of the MSIOs. Therefore, the in vivo toxicity assessments of the MSIOs including the histological sliced analysis, biodistribution and body weight, were conducted. BALB/C tumor-bearing mice were i.v. injected with a dose of MSIOs of 20 mg/kg of body weight (200 μL, 2 mg/mL). Compared with the control group, the histological examination of hematoxylin and eosin (H&E) stained slices of the major organs such as heart, liver, spleen, lung and kidney showed that there were no noticeable organ damage and inflammatory lesion for all the major organs of the mice after injection with MSIOs for 7 days (Figure 6a). Also, no necrosis was found in any of the groups. The biodistribution results in Figure 6b detected by ICP-MS indicated that the early high concentration accumulation of MSIOs in the liver and spleen was expected which was related to the clearance of foreign materials by the macrophage system within 24 h 34. At the 48 h time point, there was a slight decrease in the amount of the MSIOs in the liver and spleen, suggesting the gradually clearance from the two organs. After 7 day, notable decreases of the MSIOs in the liver and spleen were found, indicating that the MSIOs have an overt clearance from the two organs. Also, a relatively high accumulation of the MSIOs within tumor can be observed within 24 h and gradually clearance from the tumor at the 48 h and 7 day. A combined effect of a locally increased capillary permeability at the tumor site due to the EPR effect and the strong magnetic targeting effect may be responsible for the increased accumulation of MSIOs within 24 h, which was proved by the ICP-MS result (Supplementary Figure S10). Meanwhile, fluctuation of body weight is a useful indicator for investigating the toxicity effect of the MSIOs. No significant body weight loss was observed in the treated group over a period of 15 days (Figure 6c). These results proved that MSIOs at the given dose hardly cause in vivo toxicity and could be used as an injectable theranostic agent in vivo.

Bottom Line: The MoS2/Fe3O4 composite (MSIOs) functionalized by biocompatible polyethylene glycol (PEG) were prepared by a simple two-step hydrothermal method.And the as-obtained MSIOs exhibit high stability in bio-fluids and low toxicity in vitro and in vivo.Specifically, the MSIOs can be applied as a dual-modal probe for T2-weighted magnetic resonance (MR) and photoacoustic tomography (PAT) imaging due to their superparamagnetic property and strong NIR absorption.

View Article: PubMed Central - PubMed

Affiliation: 1. Key Laboratory of Polymer Science and Technology, School of Science, Northwestern Polytechnical University, Xi'an, Shaanxi, China ; 2. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Beijing, China.

ABSTRACT
The ability to selectively destroy cancer cells while sparing normal tissue is highly desirable during the cancer therapy. Here, magnetic targeted photothermal therapy was demonstrated by the integration of MoS2 (MS) flakes and Fe3O4 (IO) nanoparticles (NPs), where MoS2 converted near-infrared (NIR) light into heat and Fe3O4 NPs served as target moiety directed by external magnetic field to tumor site. The MoS2/Fe3O4 composite (MSIOs) functionalized by biocompatible polyethylene glycol (PEG) were prepared by a simple two-step hydrothermal method. And the as-obtained MSIOs exhibit high stability in bio-fluids and low toxicity in vitro and in vivo. Specifically, the MSIOs can be applied as a dual-modal probe for T2-weighted magnetic resonance (MR) and photoacoustic tomography (PAT) imaging due to their superparamagnetic property and strong NIR absorption. Furthermore, we demonstrate an effective result for magnetically targeted photothermal ablation of cancer. All these results show a great potential for localized photothermal ablation of cancer spatially/timely guided by the magnetic field and indicated the promise of the multifunctional MSIOs for applications in cancer theranostics.

No MeSH data available.


Related in: MedlinePlus