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Controlled Delivery of Human Cells by Temperature Responsive Microcapsules.

Mak WC, Olesen K, Sivlér P, Lee CJ, Moreno-Jimenez I, Edin J, Courtman D, Skog M, Griffith M - J Funct Biomater (2015)

Bottom Line: However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into.Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line-human umbilical vein endothelial cells (HUVECs).While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

View Article: PubMed Central - PubMed

Affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. mamak@ifm.liu.se.

ABSTRACT
Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into. Encapsulation of individual cells has been proposed as a means of increasing cell viability. In this study, we developed a facile, high throughput method for creating temperature responsive microcapsules comprising agarose, gelatin and fibrinogen for delivery and subsequent controlled release of cells. We verified the hypothesis that composite capsules combining agarose and gelatin, which possess different phase transition temperatures from solid to liquid, facilitated the destabilization of the capsules for cell release. Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line-human umbilical vein endothelial cells (HUVECs). While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

No MeSH data available.


Related in: MedlinePlus

Optical micrographs showing the release of human fibroblast cells onto collagen coated tissue culture dishes from microcapsules composed of (A) agarose–gelatin–fibrinogen, (B) agarose only negative controls and (C) non-encapsulated fibroblast controls. (D) Sequence of images (i) to (v) with duration of ~1.5 h showing the release of a human fibroblast cell from an agarose–gelatin–fibrinogen capsule onto the culture substratum.
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jfb-06-00439-f005: Optical micrographs showing the release of human fibroblast cells onto collagen coated tissue culture dishes from microcapsules composed of (A) agarose–gelatin–fibrinogen, (B) agarose only negative controls and (C) non-encapsulated fibroblast controls. (D) Sequence of images (i) to (v) with duration of ~1.5 h showing the release of a human fibroblast cell from an agarose–gelatin–fibrinogen capsule onto the culture substratum.

Mentions: Temperature regulated cell release was detected as a decrease in the number of cells (with rounded morphology) found within microcapsules that corresponded to an increase in the number of released cells, visualized as cells that had attached and spread on the collagen substratum (Figure 5A). Control capsules comprising agarose alone did not result in cell release (Figure 5B) and non-encapsulated fibroblasts control (Figure 5C). This confirmed our hypothesis that the gelatin was needed as a temperature responsive agent by facilitating decomposition of the capsule at physiological temperatures.


Controlled Delivery of Human Cells by Temperature Responsive Microcapsules.

Mak WC, Olesen K, Sivlér P, Lee CJ, Moreno-Jimenez I, Edin J, Courtman D, Skog M, Griffith M - J Funct Biomater (2015)

Optical micrographs showing the release of human fibroblast cells onto collagen coated tissue culture dishes from microcapsules composed of (A) agarose–gelatin–fibrinogen, (B) agarose only negative controls and (C) non-encapsulated fibroblast controls. (D) Sequence of images (i) to (v) with duration of ~1.5 h showing the release of a human fibroblast cell from an agarose–gelatin–fibrinogen capsule onto the culture substratum.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493523&req=5

jfb-06-00439-f005: Optical micrographs showing the release of human fibroblast cells onto collagen coated tissue culture dishes from microcapsules composed of (A) agarose–gelatin–fibrinogen, (B) agarose only negative controls and (C) non-encapsulated fibroblast controls. (D) Sequence of images (i) to (v) with duration of ~1.5 h showing the release of a human fibroblast cell from an agarose–gelatin–fibrinogen capsule onto the culture substratum.
Mentions: Temperature regulated cell release was detected as a decrease in the number of cells (with rounded morphology) found within microcapsules that corresponded to an increase in the number of released cells, visualized as cells that had attached and spread on the collagen substratum (Figure 5A). Control capsules comprising agarose alone did not result in cell release (Figure 5B) and non-encapsulated fibroblasts control (Figure 5C). This confirmed our hypothesis that the gelatin was needed as a temperature responsive agent by facilitating decomposition of the capsule at physiological temperatures.

Bottom Line: However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into.Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line-human umbilical vein endothelial cells (HUVECs).While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

View Article: PubMed Central - PubMed

Affiliation: Integrative Regenerative Medicine Centre, Department of Clinical and Experimental Medicine, Linköping University, SE58185, Linköping, Sweden. mamak@ifm.liu.se.

ABSTRACT
Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into. Encapsulation of individual cells has been proposed as a means of increasing cell viability. In this study, we developed a facile, high throughput method for creating temperature responsive microcapsules comprising agarose, gelatin and fibrinogen for delivery and subsequent controlled release of cells. We verified the hypothesis that composite capsules combining agarose and gelatin, which possess different phase transition temperatures from solid to liquid, facilitated the destabilization of the capsules for cell release. Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line-human umbilical vein endothelial cells (HUVECs). While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

No MeSH data available.


Related in: MedlinePlus