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Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union.

Behrends DA, Khendek L, Gao C, Zayed N, Henderson JE, Martineau PA - J Funct Biomater (2015)

Bottom Line: A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation.With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging.The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality.

View Article: PubMed Central - PubMed

Affiliation: Bone Engineering Labs, Research Institute-McGill University Health Centre, Montreal General Hospital, 1650 Cedar Ave, Montreal, Quebec H3G 1A4, Canada. domi.behrends@gmail.com.

ABSTRACT
A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid's small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment modalities before their translation into clinical practice. The goal of this study was to model a human scaphoid fracture complicated by non-union in Yucatan mini-pigs. Imaging and perfusion studies were used to confirm that the anatomy and blood supply of the radiocarpal bone in mini-pigs were similar to the human scaphoid. A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation. Bone healing was assessed using quantitative micro computed tomography and histology. With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging. The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality. This model is an appropriate, easily reproducible model for the evaluation of novel approaches for the repair of human scaphoid fractures.

No MeSH data available.


Related in: MedlinePlus

Quantitative micro CT analysis of repair after immediate (IF) and delayed (DF) fixation: The osteotomy site and adjacent bone in the LEFT (IF) and RIGHT (DF) radiocarpal bones of minipigs Abigail, Dorothy and May were scanned at 19 weeks post-operative and the data analysed using CtAn software. Quantitative data for bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp) and trabecular connectivity (Conn.Dn) are shown for the dorsal (square), osteotomy site (circle) and volar (triangle) aspects of the bone. Delayed fixation resulted in significantly less bone with poor quality at the osteotomy site in all animals.
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jfb-06-00407-f004: Quantitative micro CT analysis of repair after immediate (IF) and delayed (DF) fixation: The osteotomy site and adjacent bone in the LEFT (IF) and RIGHT (DF) radiocarpal bones of minipigs Abigail, Dorothy and May were scanned at 19 weeks post-operative and the data analysed using CtAn software. Quantitative data for bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp) and trabecular connectivity (Conn.Dn) are shown for the dorsal (square), osteotomy site (circle) and volar (triangle) aspects of the bone. Delayed fixation resulted in significantly less bone with poor quality at the osteotomy site in all animals.

Mentions: In contrast to immediate fixation (IF) (Figure 3A–G), where bone was seen adjacent to the screw along its entire length, delayed fixation (DF) resulted in soft tissue along the length of the screw (Figure 3A’–G’). This is most noticeable on the histological sections where bone is red and soft tissue blue. Quantitative micro CT (Figure 4) revealed significantly less bone (BV/TV) adjacent to the screw at the osteotomy site (white circle in Figure 3B) with DF compared with IF.


Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union.

Behrends DA, Khendek L, Gao C, Zayed N, Henderson JE, Martineau PA - J Funct Biomater (2015)

Quantitative micro CT analysis of repair after immediate (IF) and delayed (DF) fixation: The osteotomy site and adjacent bone in the LEFT (IF) and RIGHT (DF) radiocarpal bones of minipigs Abigail, Dorothy and May were scanned at 19 weeks post-operative and the data analysed using CtAn software. Quantitative data for bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp) and trabecular connectivity (Conn.Dn) are shown for the dorsal (square), osteotomy site (circle) and volar (triangle) aspects of the bone. Delayed fixation resulted in significantly less bone with poor quality at the osteotomy site in all animals.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493521&req=5

jfb-06-00407-f004: Quantitative micro CT analysis of repair after immediate (IF) and delayed (DF) fixation: The osteotomy site and adjacent bone in the LEFT (IF) and RIGHT (DF) radiocarpal bones of minipigs Abigail, Dorothy and May were scanned at 19 weeks post-operative and the data analysed using CtAn software. Quantitative data for bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp) and trabecular connectivity (Conn.Dn) are shown for the dorsal (square), osteotomy site (circle) and volar (triangle) aspects of the bone. Delayed fixation resulted in significantly less bone with poor quality at the osteotomy site in all animals.
Mentions: In contrast to immediate fixation (IF) (Figure 3A–G), where bone was seen adjacent to the screw along its entire length, delayed fixation (DF) resulted in soft tissue along the length of the screw (Figure 3A’–G’). This is most noticeable on the histological sections where bone is red and soft tissue blue. Quantitative micro CT (Figure 4) revealed significantly less bone (BV/TV) adjacent to the screw at the osteotomy site (white circle in Figure 3B) with DF compared with IF.

Bottom Line: A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation.With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging.The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality.

View Article: PubMed Central - PubMed

Affiliation: Bone Engineering Labs, Research Institute-McGill University Health Centre, Montreal General Hospital, 1650 Cedar Ave, Montreal, Quebec H3G 1A4, Canada. domi.behrends@gmail.com.

ABSTRACT
A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid's small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment modalities before their translation into clinical practice. The goal of this study was to model a human scaphoid fracture complicated by non-union in Yucatan mini-pigs. Imaging and perfusion studies were used to confirm that the anatomy and blood supply of the radiocarpal bone in mini-pigs were similar to the human scaphoid. A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation. Bone healing was assessed using quantitative micro computed tomography and histology. With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging. The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality. This model is an appropriate, easily reproducible model for the evaluation of novel approaches for the repair of human scaphoid fractures.

No MeSH data available.


Related in: MedlinePlus