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Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union.

Behrends DA, Khendek L, Gao C, Zayed N, Henderson JE, Martineau PA - J Funct Biomater (2015)

Bottom Line: A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation.With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging.The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality.

View Article: PubMed Central - PubMed

Affiliation: Bone Engineering Labs, Research Institute-McGill University Health Centre, Montreal General Hospital, 1650 Cedar Ave, Montreal, Quebec H3G 1A4, Canada. domi.behrends@gmail.com.

ABSTRACT
A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid's small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment modalities before their translation into clinical practice. The goal of this study was to model a human scaphoid fracture complicated by non-union in Yucatan mini-pigs. Imaging and perfusion studies were used to confirm that the anatomy and blood supply of the radiocarpal bone in mini-pigs were similar to the human scaphoid. A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation. Bone healing was assessed using quantitative micro computed tomography and histology. With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging. The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality. This model is an appropriate, easily reproducible model for the evaluation of novel approaches for the repair of human scaphoid fractures.

No MeSH data available.


Related in: MedlinePlus

Surgical intervention to generate radiocarpal non-union: X-rays of the forearms of anesthetized mini-pigs were obtained in the coronal (A) and sagittal (B) planes to measure the dimensions (dotted lines) of the radiocarpal bones prior to surgery. Medial-Lateral = 7.4 mm; Volar-Dorsal = 17.5 mm and Proximal-Distal 10.5 mm. 3 mm osteotomies (C arrow) were performed through the mid-radiocarpal bone of both forelimbs. The volar and dorsal pieces of the LEFT radiocarpal (control) were fixed immediately and a dense-collagen spacer (D arrow) inserted in the gap of the RIGHT radiocarpal (non-union). After 7 weeks of healing the LEFT radiocarpal had no further intervention while the spacer in the RIGHT radiocarpal was removed, the volar and dorsal segments re-apposed and fixed with a screw (E). The mini-pigs were left for an addition 12 weeks before termination of the experiment.
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jfb-06-00407-f002: Surgical intervention to generate radiocarpal non-union: X-rays of the forearms of anesthetized mini-pigs were obtained in the coronal (A) and sagittal (B) planes to measure the dimensions (dotted lines) of the radiocarpal bones prior to surgery. Medial-Lateral = 7.4 mm; Volar-Dorsal = 17.5 mm and Proximal-Distal 10.5 mm. 3 mm osteotomies (C arrow) were performed through the mid-radiocarpal bone of both forelimbs. The volar and dorsal pieces of the LEFT radiocarpal (control) were fixed immediately and a dense-collagen spacer (D arrow) inserted in the gap of the RIGHT radiocarpal (non-union). After 7 weeks of healing the LEFT radiocarpal had no further intervention while the spacer in the RIGHT radiocarpal was removed, the volar and dorsal segments re-apposed and fixed with a screw (E). The mini-pigs were left for an addition 12 weeks before termination of the experiment.

Mentions: High resolution X-rays taken pre-operatively were used to confirm the dimensions of each radiocarpal bone prior to surgical intervention, and post-operative to ensure adequate fixation of the defect (Figure 2).


Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union.

Behrends DA, Khendek L, Gao C, Zayed N, Henderson JE, Martineau PA - J Funct Biomater (2015)

Surgical intervention to generate radiocarpal non-union: X-rays of the forearms of anesthetized mini-pigs were obtained in the coronal (A) and sagittal (B) planes to measure the dimensions (dotted lines) of the radiocarpal bones prior to surgery. Medial-Lateral = 7.4 mm; Volar-Dorsal = 17.5 mm and Proximal-Distal 10.5 mm. 3 mm osteotomies (C arrow) were performed through the mid-radiocarpal bone of both forelimbs. The volar and dorsal pieces of the LEFT radiocarpal (control) were fixed immediately and a dense-collagen spacer (D arrow) inserted in the gap of the RIGHT radiocarpal (non-union). After 7 weeks of healing the LEFT radiocarpal had no further intervention while the spacer in the RIGHT radiocarpal was removed, the volar and dorsal segments re-apposed and fixed with a screw (E). The mini-pigs were left for an addition 12 weeks before termination of the experiment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493521&req=5

jfb-06-00407-f002: Surgical intervention to generate radiocarpal non-union: X-rays of the forearms of anesthetized mini-pigs were obtained in the coronal (A) and sagittal (B) planes to measure the dimensions (dotted lines) of the radiocarpal bones prior to surgery. Medial-Lateral = 7.4 mm; Volar-Dorsal = 17.5 mm and Proximal-Distal 10.5 mm. 3 mm osteotomies (C arrow) were performed through the mid-radiocarpal bone of both forelimbs. The volar and dorsal pieces of the LEFT radiocarpal (control) were fixed immediately and a dense-collagen spacer (D arrow) inserted in the gap of the RIGHT radiocarpal (non-union). After 7 weeks of healing the LEFT radiocarpal had no further intervention while the spacer in the RIGHT radiocarpal was removed, the volar and dorsal segments re-apposed and fixed with a screw (E). The mini-pigs were left for an addition 12 weeks before termination of the experiment.
Mentions: High resolution X-rays taken pre-operatively were used to confirm the dimensions of each radiocarpal bone prior to surgical intervention, and post-operative to ensure adequate fixation of the defect (Figure 2).

Bottom Line: A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation.With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging.The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality.

View Article: PubMed Central - PubMed

Affiliation: Bone Engineering Labs, Research Institute-McGill University Health Centre, Montreal General Hospital, 1650 Cedar Ave, Montreal, Quebec H3G 1A4, Canada. domi.behrends@gmail.com.

ABSTRACT
A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid's small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment modalities before their translation into clinical practice. The goal of this study was to model a human scaphoid fracture complicated by non-union in Yucatan mini-pigs. Imaging and perfusion studies were used to confirm that the anatomy and blood supply of the radiocarpal bone in mini-pigs were similar to the human scaphoid. A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation. Bone healing was assessed using quantitative micro computed tomography and histology. With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging. The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality. This model is an appropriate, easily reproducible model for the evaluation of novel approaches for the repair of human scaphoid fractures.

No MeSH data available.


Related in: MedlinePlus