Limits...
Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates.

Shin YC, Lee JH, Kim MJ, Park JH, Kim SE, Kim JS, Oh JW, Han DW - J Funct Biomater (2015)

Bottom Line: RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry.Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM.These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea. choel15@naver.com.

ABSTRACT
In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

No MeSH data available.


Related in: MedlinePlus

Proliferation of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and HASMCs on the controls (TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05. (A) RAW 264.7 cells; (B) MC3T3-E1 cells; (C) MG-63 cells; (D) HASMCs.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4493517&req=5

jfb-06-00367-f005: Proliferation of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and HASMCs on the controls (TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05. (A) RAW 264.7 cells; (B) MC3T3-E1 cells; (C) MG-63 cells; (D) HASMCs.

Mentions: We examined the proliferation of the four types of cells on the RGD-PLGA nanofiber sheets on 1, 3, 5 and 7 days after culture. The proliferation of all cells was increased with culture time (Figure 5). However, the proliferation of all cells was significantly (p < 0.05) increased on the RGD-PLGA nanofiber sheets by comparison with that on the pure PLGA nanofiber sheets and controls (tissue culture plastics, TCPs). These results correspond closely with previous findings showing that cell growth is effectively promoted on the RGD peptide-decorated substrates [16,17,21,36]. In addition, according to the previous literature, the promoted proliferation of cells on the RGD-PLGA nanofiber sheets could be attributed to an increase in the initial adhesion on the sheets [17]. Thus, it was found that the RGD-PLGA nanofiber sheets are highly effective at promoting the proliferation and growth of cells.


Biomimetic Hybrid Nanofiber Sheets Composed of RGD Peptide-Decorated PLGA as Cell-Adhesive Substrates.

Shin YC, Lee JH, Kim MJ, Park JH, Kim SE, Kim JS, Oh JW, Han DW - J Funct Biomater (2015)

Proliferation of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and HASMCs on the controls (TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05. (A) RAW 264.7 cells; (B) MC3T3-E1 cells; (C) MG-63 cells; (D) HASMCs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4493517&req=5

jfb-06-00367-f005: Proliferation of RAW 264.7 cells, MC3T3-E1 cells, MG-63 cells and HASMCs on the controls (TCPs), PLGA nanofiber sheets and RGD-PLGA nanofiber sheets. An asterisk (*) denotes a significant difference between the controls and RGD-PLGA nanofiber sheets, p < 0.05. (A) RAW 264.7 cells; (B) MC3T3-E1 cells; (C) MG-63 cells; (D) HASMCs.
Mentions: We examined the proliferation of the four types of cells on the RGD-PLGA nanofiber sheets on 1, 3, 5 and 7 days after culture. The proliferation of all cells was increased with culture time (Figure 5). However, the proliferation of all cells was significantly (p < 0.05) increased on the RGD-PLGA nanofiber sheets by comparison with that on the pure PLGA nanofiber sheets and controls (tissue culture plastics, TCPs). These results correspond closely with previous findings showing that cell growth is effectively promoted on the RGD peptide-decorated substrates [16,17,21,36]. In addition, according to the previous literature, the promoted proliferation of cells on the RGD-PLGA nanofiber sheets could be attributed to an increase in the initial adhesion on the sheets [17]. Thus, it was found that the RGD-PLGA nanofiber sheets are highly effective at promoting the proliferation and growth of cells.

Bottom Line: RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry.Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM.These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea. choel15@naver.com.

ABSTRACT
In biomedical applications, there is a need for tissue engineering scaffolds to promote and control cellular behaviors, including adhesion, proliferation and differentiation. In particular, the initial adhesion of cells has a great influence on those cellular behaviors. In this study, we concentrate on developing cell-adhesive substrates applicable for tissue engineering scaffolds. The hybrid nanofiber sheets were prepared by electrospinning poly(lactic-co-glycolic acid) (PLGA) and M13 phage, which was genetically modified to enhance cell adhesion thru expressing RGD peptides on their surface. The RGD peptide is a specific motif of extracellular matrix (ECM) for integrin receptors of cells. RGD peptide-decorated PLGA (RGD-PLGA) nanofiber sheets were characterized by scanning electron microscopy, immunofluorescence staining, contact angle measurement and differential scanning calorimetry. In addition, the initial adhesion and proliferation of four different types of mammalian cells were determined in order to evaluate the potential of RGD-PLGA nanofiber sheets as cell-adhesive substrates. Our results showed that the hybrid nanofiber sheets have a three-dimensional porous structure comparable to the native ECM. Furthermore, the initial adhesion and proliferation of cells were significantly enhanced on RGD-PLGA sheets. These results suggest that biomimetic RGD-PLGA nanofiber sheets can be promising cell-adhesive substrates for application as tissue engineering scaffolds.

No MeSH data available.


Related in: MedlinePlus